公开(公告)号：US20200071672A1

公开(公告)日：2020-03-05

申请号：US16490302

申请日：2018-03-03

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaoyu Zhang ,  Yama A Abassi ,  Nan Li ,  Xiaobo Wang

IPC: C12N5/077 ,  C12N13/00 ,  G01N27/02 ,  C12N5/074 ,  C12N5/00

Abstract: Methods of inducing functional maturation of immature cardiomyocytes derived from induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) by electrically pacing the immature cardiomyocytes according to a pulse profile that induces maturation until the immature cardiomyocytes mature into functionally adult cardiomyocytes.

公开(公告)号：US20180246079A1

公开(公告)日：2018-08-30

申请号：US15523649

申请日：2016-11-21

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Yama A Abassi ,  Nan Li ,  Lincoln Muir ,  Xiao Xu

IPC: G01N33/50 ,  C12N13/00 ,  C12M1/34 ,  G01N33/487

CPC classification number: G01N33/5011 ,  C12M1/3407 ,  C12N13/00 ,  G01N33/4836 ,  G01N33/48707 ,  G01N33/502 ,  G01N33/5047 ,  G01N2800/52 ,  G01N2800/7028

Abstract: Methods of assessing cytolysis of cancer cells, including providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, wherein the device comprises a well for receiving cells and an electrode array at a base of the well; adding target cells characterized as cancer cells to the well; adding effector cells to the well to form a test well, wherein the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance based parameter over time.