公开(公告)号：US20070252988A1

公开(公告)日：2007-11-01

申请号：US11202211

申请日：2005-08-12

Applicant: Jeremy Levy

Inventor： Jeremy Levy

IPC: G01J3/06 ,  G01J3/28

CPC classification number: G01J3/2823 ,  G01J3/0294 ,  G01J3/1256 ,  G01J3/2803 ,  G01J3/2889 ,  G01J3/433 ,  G01J2001/4242

Abstract: The development of a multiple-channel dual phase lock-in optical spectrometer (LIOS) is presented, which enables parallel phase-sensitive detection at the output of an optical spectrometer. The light intensity from a spectrally broad source is modulated at the reference frequency, and focused into a high-resolution imaging spectrometer. The height at which the light enters the spectrometer is controlled by an acousto-optic deflector, and the height information is preserved at the output focal plane. A two-dimensional InGaAs focal plane array collects light that has been dispersed in wavelength along the horizontal direction, and in time along the vertical direction. The data is demodulated using a high performance computer-based digital signal processor. This parallel approach greatly enhances (by more than 100×) the speed at which spectrally resolved lock-in data can be acquired.

Abstract translation: 提出了一种多通道双相锁相光谱仪（LIOS）的开发，可在光谱仪的输出端进行并行相敏检测。 来自光谱宽源的光强度以参考频率调制，并且聚焦成高分辨率成像光谱仪。 光进入光谱仪的高度由声光偏转器控制，高度信息保留在输出焦平面。 二维InGaAs焦平面阵列沿着水平方向并沿着垂直方向在时间上收集沿波长分散的光。 使用基于高性能计算机的数字信号处理器对数据进行解调。 这种并行方法极大地提高了可以获取光谱解析的锁定数据的速度（超过100倍）。

公开(公告)号：US07277169B2

公开(公告)日：2007-10-02

申请号：US11355387

申请日：2006-02-16

Applicant: Jing Yong Ye ,  Theodore B. Norris ,  James R. Baker, Jr.

Inventor： Jing Yong Ye ,  Theodore B. Norris ,  James R. Baker, Jr.

IPC: G01N21/64

CPC classification number: G01J3/4406 ,  G01J3/10 ,  G01J3/2889 ,  G01N21/6408 ,  G01N21/6458

Abstract: A fluorescence detection system for testing a sample having at least one fluorophore. The fluorescence detection system comprises a white light generation system outputting a white light pulse. The white light pulse has a first frequency range and a first time duration. The white light pulse excites the at least one fluorophore of the sample to emit a fluorescence. The fluorescence has a second frequency range and a second time duration, wherein the first time duration is less than the second time duration. A time-resolving detector receives the fluorescence and at least a portion of the white light pulse and separates the fluorescence from the portion of the white light pulse.

Abstract translation: 一种用于测试具有至少一个荧光团的样品的荧光检测系统。 荧光检测系统包括输出白光脉冲的白光发生系统。 白光脉冲具有第一频率范围和第一持续时间。 白光脉冲激发样品的至少一个荧光团以发射荧光。 荧光具有第二频率范围和第二持续时间，其中第一持续时间小于第二持续时间。 时间分辨检测器接收荧光和白光脉冲的至少一部分，并将荧光与白光脉冲的该部分分离。

公开(公告)号：US20070223006A1

公开(公告)日：2007-09-27

申请号：US11624455

申请日：2007-01-18

Applicant: Guillermo Tearney ,  Brett Bouma ,  Jason Motz ,  Leilei Peng

Inventor： Guillermo Tearney ,  Brett Bouma ,  Jason Motz ,  Leilei Peng

IPC: G01B11/02

CPC classification number: G01N21/6408 ,  G01J3/10 ,  G01J3/2889 ,  G01J3/433 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6445 ,  G01N2021/6417 ,  G01N2021/6419

Abstract: Exemplary systems and methods for obtaining information associated with at least one portion of a sample can be provided. For example, a first radiation can be received and at least one second radiation and at least one third radiation can be provided as a function of the first radiation. Respective intensities of the second and third radiations can be modulated, whereas the second and third radiations may have different modulation frequencies, and the modulated second and third radiations can be directed toward the portion. The photoluminescence radiation can be received from the portion based on the modulated second and third radiations to generate a resultant signal. The signal can be processed to obtain the information which is/are photoluminescence lifetime characteristics and/or a polarization anisotropy of the portion. According to another exemplary embodiment, the photoluminescence radiation can be received and the photoluminescence radiation may be based on wavelengths thereof.

Abstract translation: 可以提供用于获得与样品的至少一部分相关联的信息的示例性系统和方法。 例如，可以接收第一辐射，并且可以根据第一辐射提供至少一个第二辐射和至少一个第三辐射。 可以调制第二和第三辐射的相应强度，而第二和第三辐射可以具有不同的调制频率，并且调制的第二和第三辐射可以指向该部分。 可以基于调制的第二和第三辐射从该部分接收光致发光辐射以产生结果信号。 可以处理该信号以获得该部分的光致发光寿命特性和/或偏振各向异性的信息。 根据另一示例性实施例，可以接收光致发光辐射，并且光致发光辐射可以基于其波长。

公开(公告)号：US07184144B2

公开(公告)日：2007-02-27

申请号：US10637472

申请日：2003-08-08

Applicant: Scott T. Sanders

Inventor： Scott T. Sanders

IPC: G01J3/28

CPC classification number: G01J3/2889 ,  G01J3/4338

Abstract: A high scan rate spectroscopic system converts a narrow-band laser pulse into a multispectral pulse, using, for example, a nonlinear fiber. The multispectral pulse is then converted to a swept frequency pulse through a second fiber impressing a frequency-dependent delay in the light beam which is then applied to the object to be tested.

Abstract translation: 高扫描速率光谱系统使用例如非线性光纤将窄带激光脉冲转换成多光谱脉冲。 然后，多光谱脉冲通过第二光纤转换成扫描频率脉冲，该第二光纤施加光束中的频率相关延迟，然后将其施加到待测对象。

公开(公告)号：US20060126062A1

公开(公告)日：2006-06-15

申请号：US11268590

申请日：2005-11-08

Applicant: David Tuschel

Inventor： David Tuschel

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01J3/2889 ,  G01J3/44 ,  G01N21/3563 ,  G01N21/3577 ,  G01N21/65

Abstract: The present disclosure describes methods and apparatus to produce a streaming image of a sample during a time period when an attribute of the sample is changing. The streaming image can be viewed in such a manner so as to be able to follow a visible change in an attribute of the sample. The sample may be undergoing nucleation, aggregation, or chemical interaction. The present disclosure also describes methods and apparatus to determine a change in an attribute of a sample by detecting, analyzing, and comparing spectra of the sample taken at different times during the time period when the attribute of the sample is changing. The sample may be undergoing nucleation, aggregation, or chemical interaction.

Abstract translation: 本公开描述了在样本的属性改变的时间段期间产生样本的流图像的方法和装置。 可以以这样的方式来观看流图像，以便能够跟踪样本的属性的可见变化。 样品可能经历成核，聚集或化学相互作用。 本公开还描述了通过检测，分析和比较在样本的属性正在改变的时间段期间在不同时间拍摄的样本的光谱来确定样本的属性变化的方法和装置。 样品可能经历成核，聚集或化学相互作用。

公开(公告)号：US20060068371A1

公开(公告)日：2006-03-30

申请号：US11123610

申请日：2005-05-04

Applicant: William Ortyn ,  David Basiji ,  David Lynch

Inventor： William Ortyn ,  David Basiji ,  David Lynch

IPC: C12Q1/00 ,  G01N21/25

CPC classification number: G01N33/505 ,  G01J3/2889 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/5005 ,  G01N2015/1006 ,  G01N2015/1497 ,  G01N2021/6421 ,  G01N2021/6441 ,  G06K9/00127

Abstract: Aspects of the present invention encompass the collection of multispectral images from a population of objects, and the analysis of the collected images to measure at least characteristic of the population, using photometric and/or morphometric features identifiable in the collection of images. In an exemplary application, the objects are biological cells. In a particularly preferred, but not limiting implementation, the plurality of images for each individual object are collected simultaneously. In an empirical study the characteristic being measured involves the synapse between conjugated cells. The conjugated cells may represent a subpopulation of the overall population of objects that were imaged. In a particularly preferred, yet not limiting embodiment, the present invention enables the quantization of the redistribution of cellular molecules due to the conjugation of different biological cells. Significantly, such quantization is not feasible with standard microscopy and flow cytometry.

Abstract translation: 本发明的方面包括从对象群体收集多光谱图像，以及使用在图像集合中可识别的光度和/或形态测量特征，对所收集的图像的分析至少测量群体的特征。 在示例性应用中，对象是生物细胞。 在特别优选但不是限制的实现中，用于每个单独对象的多个图像被同时收集。 在经验研究中，被测量的特征涉及共轭细胞之间的突触。 共轭细胞可以代表成像对象的总体群体的亚群。 在特别优选的但不是限制性的实施方案中，本发明使得由于不同生物细胞的共轭而能够量化细胞分子的再分布。 值得注意的是，这种量化在标准显微镜和流式细胞术中是不可行的。

公开(公告)号：US20050058352A1

公开(公告)日：2005-03-17

申请号：US10892740

申请日：2004-07-16

Applicant: Shrenik Deliwala

Inventor： Shrenik Deliwala

IPC: G01J1/00 ,  G06K20060101 ,  G06K9/36

CPC classification number: G01J3/2823 ,  G01J3/02 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/2889 ,  G01J3/433 ,  G01N21/211 ,  G01N2021/213

Abstract: The invention features methods including: (i) modulating multiple components (e.g., different spatial regions, different wavelengths, or both) of electromagnetic (EM) radiation emerging from an object with different time-varying functions; (ii) measuring at least one time-varying signal derived from the modulated EM radiation emerging from the object; and (iii) reconstructing information about the object based on the measured signal and the time-varying functions.

Abstract translation: 本发明的特征在于方法包括：（i）调制从具有不同时变功能的物体出射的电磁（EM）辐射的多个分量（例如，不同的空间区域，不同的波长或两者） （ii）测量从所述对象中出现的经调制的EM辐射得到的至少一个时变信号; 和（iii）基于所测量的信号和随时间变化的功能重建关于对象的信息。

公开(公告)号：US20050012925A1

公开(公告)日：2005-01-20

申请号：US10840686

申请日：2004-05-06

Applicant: Vidi Saptari ,  Kamal Youcef-Toumi

Inventor： Vidi Saptari ,  Kamal Youcef-Toumi

IPC: G01J3/06 ,  G01J3/12 ,  G01J3/28 ,  G01J3/42 ,  G01J3/433 ,  G01N21/25 ,  G01N21/27 ,  G01N21/31 ,  G01N21/35

CPC classification number: G01J3/06 ,  G01J3/2889 ,  G01J3/42 ,  G01J3/433 ,  G01J2003/062 ,  G01J2003/1243 ,  G01N21/255 ,  G01N21/274 ,  G01N21/276 ,  G01N21/3504 ,  G01N2021/317

Abstract: The present invention relates to spectroscopic methods and systems for collecting electromagnetic radiation from an object using a continuously-spinning wavelength-selecting (CSWS) device, e.g., an interference filter(s)?? or grating. One embodiment of the invention provides a spectroscopic system for collecting electromagnetic radiation from a target. The spectroscopic system has at least one beam of electromagnetic radiation that interacts with the target. The system includes a continuously spinning wavelength-selecting (CSWS) device, e.g., a continuously spinning interference filter/grating driven by a DC motor, in the path of the at least one beam. The device filters the radiation with regard to wavelength to produce filtered radiation. The system further includes at least one detector in the path of the at least one beam for detecting the filtered radiation.

Abstract translation: 本发明涉及使用连续纺丝波长选择（CSWS）装置（例如干涉滤光片）收集来自物体的电磁辐射的光谱方法和系统。 或光栅。 本发明的一个实施例提供了一种用于从目标收集电磁辐射的光谱系统。 光谱系统具有与靶相互作用的至少一束电磁辐射。 该系统包括连续旋转波长选择（CSWS）装置，例如由直流电动机驱动的连续旋转干涉滤波器/光栅，在至少一个光束的路径中。 该装置相对于波长对辐射进行滤波以产生滤波的辐射。 该系统还包括至少一个光束的路径中的至少一个检测器，用于检测经过滤的辐射。

公开(公告)号：US20020044272A1

公开(公告)日：2002-04-18

申请号：US09932844

申请日：2001-08-17

Applicant: Amnis Corporation

Inventor： David A. Basiji ,  William E. Ortyn

IPC: G01N021/00

CPC classification number: G06K9/00127 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0232 ,  G01J3/14 ,  G01J3/2823 ,  G01J3/2889 ,  G01N15/14 ,  G01N21/6458 ,  G01N2015/1497

Abstract: Light from an object moving through an imaging system is collected, dispersed, and imaged onto a time delay integration (TDI) detector that is inclined relative to an axis of motion of the object, producing a pixilated output signal. In one embodiment, the movement of the image object over the TDI detector is asynchronous with the movement of the output signal producing an output signal that is a composite of the image of the object at varying focal point along the focal plane. In another embodiment, light from the object is periodically incident on the inclined TDI detector, producing a plurality of spaced apart images and corresponding output signals that propagate across the TDI detector. The inclined plane enables images of FISH probes or other components within an object to be produced at different focal points, so that the 3D spatial relationship between the FISH probes or components can be resolved.

Abstract translation: 来自移动通过成像系统的物体的光被收集，分散和成像到相对于物体的运动轴线倾斜的时间延迟积分（TDI）检测器，产生像素化的输出信号。 在一个实施例中，图像对象在TDI检测器上的移动与产生输出信号的输出信号的移动是异步的，该输出信号是沿着焦平面的变化焦点处的物体的图像的复合。 在另一个实施例中，来自物体的光周期性地入射到倾斜的TDI检测器上，产生多个间隔开的图像和在TDI检测器上传播的对应的输出信号。 倾斜平面使得可以在不同焦点处产生物体内的FISH探针或其他部件的图像，从而可以解决FISH探针或部件之间的3D空间关系。

公开(公告)号：US06192260B1

公开(公告)日：2001-02-20

申请号：US07876364

申请日：1992-04-30

Applicant: Britton Chance

Inventor： Britton Chance

IPC: A61B310

CPC classification number: G01J3/2889 ,  G01N21/49 ,  G01N21/59 ,  G01N2021/1791

Abstract: Methods and apparatus using the principles of time-resolved spectroscopy are disclosed. The present invention employs incident light pulses of sufficiently short duration to permit the rate of the rise and decay of such pulses to be measured. Consequently, the rate of decay, u, permits a determination of the concentration of an absorptive pigment, such as hemoglobin. The present invention also allows the precise path length the photons travel to be determined. Using this path length information and by measuring changes in optical density using known continuous light (CW) spectrophotometry systems, the methods and apparatus disclosed allow changes in the concentration of an absorptive pigment to be correctly be measured. From these data, the oxygenation state of a tissue region, such as the brain, can be accurately determined in real time.

Abstract translation: 公开了使用时间分辨光谱原理的方法和装置。 本发明采用足够短持续时间的入射光脉冲，以允许测量这些脉冲的上升和下降的速率。 因此，衰减速率u允许测定吸收性颜料如血红蛋白的浓度。 本发明还允许确定光子行进的精确路径长度。 使用该路径长度信息并通过使用已知的连续光（CW）分光光度法系统测量光密度的变化，所公开的方法和装置允许正确测量吸收性颜料的浓度的变化。 从这些数据可以准确地确定脑组织区域的氧合状态。