公开(公告)号：US20230331763A1

公开(公告)日：2023-10-19

申请号：US18176609

申请日：2023-03-01

Applicant: Verve Therapeutics, Inc.

Inventor： Christopher Cheng ,  Kallanthottathil Rajeev ,  Caroline Reiss ,  Kui Wang ,  Athul Sanjeev

IPC: C07H21/02 ,  C12P19/34 ,  C12N9/12

CPC classification number: C07H21/02 ,  C12P19/34 ,  C12N9/1247 ,  C12Y207/07006

Abstract: Provided herein are compositions and methods for preparation of 5′ end region-modified mRNAs. In particular, the instant disclosure relates to novel mRNA 5′ end region motifs and sequence initiators therefore together with assays that are capable of measuring the aspects of the functionality of those motifs and sequence initiators. Further provided herein are compositions and methods of treating conditions related to coronary disease.

公开(公告)号：US20230295625A1

公开(公告)日：2023-09-21

申请号：US18148396

申请日：2022-12-29

Applicant: DNA TWOPOINTO INC.

Inventor： Jeremy MINSHULL ,  Ferenc BOLDOG ,  Varsha SITARAMAN ,  Maggie LEE

IPC: C12N15/113 ,  C12N9/12 ,  C12N15/86 ,  C12N15/90

CPC classification number: C12N15/113 ,  C12N9/1241 ,  C12N9/1247 ,  C12N15/86 ,  C12N15/90 ,  C12N15/1137 ,  C12N2310/51 ,  C12N2310/141 ,  C12N2310/531 ,  C12N2310/3519 ,  C12N2800/90 ,  C12Y207/07006 ,  C12Y603/01002

Abstract: The present invention provides methods and compositions for stable genetic modification of cultured mammalian cells. The genetic modifications can be used to produce- cultured mammalian cells for therapeutic of diagnostic purposes.

公开(公告)号：US11753670B1

公开(公告)日：2023-09-12

申请号：US17862495

申请日：2022-07-12

Applicant: U.S. Army Combat Capabilities Development Command, Chemical Biological Center

Inventor： Aleksandr E. Miklos ,  Katherine A. Rhea ,  Nathan D. McDonald ,  Stephanie D. Cole ,  Patricia E. Buckley

IPC: C12P21/02 ,  C12N9/12 ,  C12N15/74

CPC classification number: C12P21/02 ,  C12N9/1247 ,  C12N15/74 ,  C12Y207/07006 ,  C12N2800/101

Abstract: Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from Yersinia pestis species such as cellular extracts from Yersinia pestis.

公开(公告)号：US10080794B2

公开(公告)日：2018-09-25

申请号：US14777450

申请日：2014-03-15

Applicant: University of Maryland

Inventor： Daniel R. Perez ,  Matthew Angel

IPC: A61K39/145 ,  A61K39/12 ,  C12N7/00 ,  C12N9/12 ,  A61K39/00

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/53 ,  C12N7/00 ,  C12N9/1247 ,  C12N2760/16034 ,  C12N2760/16134 ,  C12Y207/07006

Abstract: Disclosed are compositions and methods comprising vectors and a reverse genetics competent unit comprising one or more recombinant influenza viruses. Recombinant influenza viruses comprising swine RNA polymerase I promoter are disclosed. Constructs comprising swine RNA polymerase I promoter nucleic acid sequences are also provided. Methods of inducing protecting immunity with the recombinant influenza viruses are disclosed. In certain embodiments, the reverse genetics competent unit comprises pathogenic units necessary for producing pathogens de novo from a nucleotide-based vector for influenza virus in swine.

公开(公告)号：US10034951B1

公开(公告)日：2018-07-31

申请号：US15820656

申请日：2017-11-22

Applicant: New England Biolabs, Inc.

Inventor： Bijoyita Roy ,  G. B. Robb

IPC: C12Q1/68 ,  A61K45/06 ,  A61K48/00 ,  C12P19/34

CPC classification number: A61K48/0091 ,  A61K31/7105 ,  A61K31/713 ,  C12N9/1247 ,  C12N2310/17 ,  C12P19/34 ,  C12Y207/07006

Abstract: Provided herein, among other things, is a method for producing an RNA product that has reduced immunogenicity without requiring removal of any dsRNA from the RNA product. In some embodiments, the method involves transcribing a template DNA with a thermostable RNA polymerase at a temperature of greater than 44° C.

公开(公告)号：US20180105806A1

公开(公告)日：2018-04-19

申请号：US15696246

申请日：2017-09-06

Applicant: Massachusetts Institute of Technology

Inventor： Christopher Voigt ,  Alec Andrew Nielsen

IPC: C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N9/00 ,  C12N9/12

CPC classification number: C12N15/102 ,  C12N9/1247 ,  C12N9/22 ,  C12N9/93 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/66 ,  C12N2310/20 ,  C12Y207/07006 ,  C12Y605/01001

Abstract: The invention provides a novel approach to facilitate assembly of DNA molecules. This approach utilizes RNA-guided endonucleases, capable of targeting any DNA sequence, which cleave DNA and generate DNA fragments characterized by single stranded overhangs. After annealing of complementary overhangs, DNA fragments are covalently connected, generating a single DNA molecule. In this way, the present invention combines the reliability of classic restriction-ligation techniques, removes all sequence constraints from the desired final DNA molecule, and expands the number of DNA pieces that can be assembled at once.

公开(公告)号：US09822348B2

公开(公告)日：2017-11-21

申请号：US15316232

申请日：2015-06-17

Applicant: Wacker Chemie AG

Inventor： Andreas Anton ,  Monique Janowski-Egler ,  Markus Liebscher

IPC: C12N9/12 ,  C12N15/72 ,  C12N15/70

CPC classification number: C12N9/1247 ,  C12N15/68 ,  C12N15/70 ,  C12N15/72 ,  C12N15/73 ,  C12Y207/07006

Abstract: The invention relates to an expression system comprising a prokaryotic cell which contains a polynucleotide construct that encodes a T7 RNA polymerase under the control of a T7 promoter and under the control of an inducible promoter. The invention also relates to an expression vector which contains a gene that encodes a protein to be expressed under the control of a T7 promoter, characterized in that said expression vector comprises a plasmid stabilization system.

公开(公告)号：US09816123B2

公开(公告)日：2017-11-14

申请号：US15022284

申请日：2014-09-16

Applicant: Inria Institut National De Recherche En Informatique Et En Automatique ,  Universite Joseph Fourier—Grenoble 1

Inventor： Johannes Geiselmann ,  Hidde De Jong ,  Delphine Ropers ,  Jérôme Izard

IPC: C12P7/20 ,  C12P21/00 ,  C12P21/02 ,  C12N9/12 ,  C12N15/72 ,  C12N15/70

CPC classification number: C12P21/00 ,  C12N9/1247 ,  C12N15/70 ,  C12N15/72 ,  C12P7/20 ,  C12P21/02 ,  C12Y207/07006

Abstract: The present invention relates to a method for producing a molecule of interest in bacteria which is based on a reversible growth arrest of the bacteria at the cellular growth global control system level, thus allowing an improved yield of production of said molecule of interest.

公开(公告)号：US09790531B2

公开(公告)日：2017-10-17

申请号：US15356132

申请日：2016-11-18

Applicant: ModernaTX, Inc.

Inventor： Yuxun Wang ,  Divakar Ramakrishnan ,  Antonin De Fougerolles ,  Susan Whoriskey

IPC: C12P19/34 ,  C12N9/12 ,  C12N15/52 ,  C12N15/54 ,  C12N15/63 ,  C12N15/70

CPC classification number: C12P19/34 ,  C12N9/1241 ,  C12N9/1247 ,  C12N9/1252 ,  C12Y207/07006 ,  C12Y207/07007 ,  C12Y207/0705

Abstract: The invention relates to compositions and methods for the design, evolution, preparation, and/or manufacture of enzymes for use with polynucleotides, primary transcripts and mmRNA molecules.

公开(公告)号：US20170216457A1

公开(公告)日：2017-08-03

申请号：US15514889

申请日：2015-10-01

Applicant: UNIVERSITY OF SEOUL INDUSTRY COOPERATION FOUNDATION

Inventor： Jong-Bum LEE ,  Hye-Jin KIM

IPC: A61K48/00 ,  C12N15/63 ,  A61K9/14 ,  C12P19/34

CPC classification number: A61K48/0066 ,  A61K9/14 ,  A61K48/0016 ,  A61K48/0091 ,  B82B1/00 ,  B82Y5/00 ,  C12N15/63 ,  C12N15/87 ,  C12P19/34 ,  C12Y207/07006

Abstract: Disclosed are nanoparticles that are introduced into cells and express a specific protein and a manufacturing method thereof. More particularly, the present invention relates to mRNA nanoparticles, which increase the expression of a specific protein capable of stimulating the cellular immune system to induce cellular immune responses and are thus applicable to treat a variety of diseases, do not require passage across the nuclear envelope because a desired gene is delivered not as plasmid DNA itself but in the form of mRNA, thus improving the efficiency of protein expression, and the nanoparticles are generated through a one-step process with a relatively small amount of plasmid DNA via rolling circle transcription (RCT), thereby providing a simple and economical process for gene delivery. The present invention is also concerned with such mRNA nanoparticles.