公开(公告)号：US11781175B1

公开(公告)日：2023-10-10

申请号：US17831427

申请日：2022-06-02

申请人： H42, INC.

发明人： Sandra Ann R Steyaert ,  Geert Trooskens ,  Wim Maria R. Van Criekinge ,  Adriaan Verhelle ,  Johan Irma H. Vandersmissen

IPC分类号： C12Q1/6876 ,  C12Q1/6844 ,  C12Q1/6806

CPC分类号： C12Q1/6844 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/154 ,  C12Q2600/16

摘要： We propose a method of generating an epigenetic age prediction. The method includes providing a sample extraction test kit, and receiving a sample extracted by the sample extraction test kit. The method further includes extracting DNA from the sample and processing the DNA to receive processed DNA. The method further includes amplifying a plurality of loci in the processed DNA to receive amplified DNA and processing the amplified DNA to receive a plurality of methylation values for one or more CpG sites in the plurality of loci.

公开(公告)号：US11773388B2

公开(公告)日：2023-10-03

申请号：US16907736

申请日：2020-06-22

申请人： Roche Sequencing Solutions, Inc. ,  Kapa Biosystems, Inc.

发明人： Daniel Burgess ,  Brian Christopher Godwin ,  Alexander Lovejoy ,  Bronwen Miller ,  Jo-Anne Elizabeth Penkler ,  Joseph Platzer

IPC分类号： C12N15/10 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q1/6874

CPC分类号： C12N15/1034 ,  C12N15/1072 ,  C12Q1/6813 ,  C12Q1/6874 ,  C12Q1/6876

摘要： The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.

公开(公告)号：US20230290442A1

公开(公告)日：2023-09-14

申请号：US18321106

申请日：2023-05-22

申请人： FUJIFILM Corporation

发明人： Naoko YAMAGUCHI

IPC分类号： G16B25/20 ,  C12Q1/6844 ,  C12Q1/6876

CPC分类号： G16B25/20 ,  C12Q1/6846 ,  C12Q1/6876 ,  C12Q2600/154

摘要： An object of the present invention is to provide a design method, a manufacturing method, a design device, a design program, and a recording medium of a primer for amplicon methylation sequence analysis, which can improve a design success rate of the primer. The present invention is a primer design method for amplicon methylation sequence analysis, the method having a base conversion step of converting methylatable “C” into “Y” and converting other “C” into “T” in double-stranded genomic DNA, and a primer candidate sequence selection step of selecting sequences satisfying predetermined selection conditions as primer candidate sequences, in which the methylatable C is C in a CG sequence, and the predetermined selection conditions include (1) a Tm value is within a predetermined range, (2) the number of YG sequences or CR sequences included in a partial sequence is equal to or less than a predetermined, and (3) an upper limit of the number of binding sites with a sequence outside the related region on the double-stranded genomic DNA after base conversion is equal to or less than a predetermined number that is 1 or more.

公开(公告)号：US20230279477A1

公开(公告)日：2023-09-07

申请号：US18302443

申请日：2023-04-18

申请人： 10x Genomics, Inc.

发明人： Linda Kvastad ,  Mohammadreza Mirzazadeh ,  Ludvig Ale Larsson ,  Joakim Lundeberg

IPC分类号： C12Q1/6841 ,  C12Q1/6876 ,  C12Q1/686

CPC分类号： C12Q1/6841 ,  C12Q1/6876 ,  C12Q1/686 ,  C12Q2600/178

摘要： Provided herein are methods for spatial analysis that captures non-poly(A)-containing RNA molecules, such as long non-coding RNAs and microRNAs. Methods, kits, and compositions for spatial analysis using targeted RNA capture using randomer capture probes are disclosed herein.

公开(公告)号：US11739382B2

公开(公告)日：2023-08-29

申请号：US16798911

申请日：2020-02-24

申请人： Roche Molecular Systems, Inc.

发明人： Jeffery Fong ,  Aaron T. Hamilton ,  Marintha Heil ,  Igor Kozlov ,  Ed Gustavo Marins ,  Elizabeth Marie Scott ,  Ling Wang

IPC分类号： C12Q1/6876 ,  C07H21/00 ,  G01N33/49

CPC分类号： C12Q1/6876 ,  G01N33/49 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2600/112

摘要： Methods for the rapid detection of the presence or absence of Hepatitis B Virus (HBV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, competitive blocking oligonucleotides, and probes targeting HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA) and kits are provided that are designed for the detection of HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA).

公开(公告)号：US11739379B2

公开(公告)日：2023-08-29

申请号：US17361821

申请日：2021-06-29

申请人： Oxford Nanopore Technologies PLC

发明人： Andrew John Heron ,  James Edward Graham ,  Richard Alexander Gutierrez ,  Rebecca Victoria Bowen ,  James White ,  Clive Gavin Brown ,  Daniel George Fordham

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/487

CPC分类号： C12Q1/6869 ,  C12Q1/6876 ,  G01N33/48721

摘要： The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

公开(公告)号：US20230253069A1

公开(公告)日：2023-08-10

申请号：US18135094

申请日：2023-04-14

申请人： Ha Tran

发明人： Ha Tran

IPC分类号： G16B20/00 ,  C12Q1/6869 ,  G06N5/04 ,  G06N20/00 ,  G16B40/00 ,  C12Q1/6876 ,  G06N7/01

CPC分类号： G16B20/00 ,  C12Q1/6869 ,  G06N5/04 ,  G06N20/00 ,  G16B40/00 ,  C12Q1/6876 ,  G06N7/01

摘要： Systems and methods disclosed for recommending healthy actions for a subject by using a DNA sequencer to generate genetic information; aggregating genetic information, health or beauty trend data, and health response from a patient population; deep learning with a computer to generate at least one computer implemented classifier that predicts matching health protocols, treatment regimes, or products based on the genetic information, health trend data, and health product response from a patient population; and recommending one or more actions, protocols, or products, for the subject.

公开(公告)号：US11718874B2

公开(公告)日：2023-08-08

申请号：US16170751

申请日：2018-10-25

申请人： President and Fellows of Harvard College

发明人： Evan R. Daugharthy ,  George M. Church

IPC分类号： C12Q1/6876 ,  C12Q1/682 ,  C12N15/115 ,  C12Q1/6806 ,  C12Q1/6816

CPC分类号： C12Q1/6876 ,  C12N15/115 ,  C12Q1/682 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12N2310/16 ,  C12N2320/10 ,  C12Q1/682 ,  C12Q2525/301 ,  C12Q2537/155 ,  C12Q2565/514

摘要： The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.

公开(公告)号：US11713485B2

公开(公告)日：2023-08-01

申请号：US17392325

申请日：2021-08-03

申请人： President and Fellows of Harvard College

发明人： Evan R. Daugharthy ,  George M. Church

IPC分类号： C12Q1/6876 ,  C12Q1/682 ,  C12N15/115 ,  C12Q1/6806 ,  C12Q1/6816

CPC分类号： C12Q1/6876 ,  C12N15/115 ,  C12Q1/682 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12N2310/16 ,  C12N2320/10 ,  C12Q1/682 ,  C12Q2525/301 ,  C12Q2537/155 ,  C12Q2565/514

摘要： The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.

公开(公告)号：US20230227892A1

公开(公告)日：2023-07-20

申请号：US17576865

申请日：2022-01-14

申请人： mbiomics GmbH

发明人： Johannes Benedikt Woehrstein ,  Heinrich Ernst Paul Grabmayr

IPC分类号： C12Q1/6816 ,  C12Q1/6876

CPC分类号： C12Q1/6816 ,  C12Q1/6876

摘要： The relative abundance of bacterial species in a patient’s microbiome is quantified using DNA nanostructures that fluoresce multiple colors. Immobilizing binders have binding sites with nucleotide sequences complementary to those at a primary site on rRNA subunits of each selected bacterial species. Fluorophore binders have binding sites with nucleotide sequences complementary to those at a secondary site on the rRNA subunits. The fluorophore binders for each bacterial species are attached to nanostructures that fluoresce a particular color for each bacteria. The immobilizing binders are attached to the surface of a microscopy chamber. RNA subunits are extracted from a microbiome sample of the patient and are attached to the corresponding immobilizing binders and fluorophore binders such that the RNA subunits of each bacterial species fluoresce a color unique to the species. DNA nanostructures emitting the same color are counted to determine the relative concentration of the bacterial species in the sample.