公开(公告)号：US20180229239A1

公开(公告)日：2018-08-16

申请号：US15894836

申请日：2018-02-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Trey CAULEY ,  John DZENITIS ,  Steve HOBBS ,  Stefano SCHIAFFINO

IPC: B01L3/00 ,  G01N1/38 ,  B01F3/08 ,  B01F13/10

CPC classification number: B01L3/502784 ,  B01F3/0811 ,  B01F13/1072 ,  B01F2215/0037 ,  B01L3/502715 ,  B01L9/527 ,  B01L2200/028 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2400/049 ,  G01N1/38

Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.

公开(公告)号：US20240012017A1

公开(公告)日：2024-01-11

申请号：US18347549

申请日：2023-07-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Steve HOBBS ,  Darren R. LINK ,  Stuart YOUNG ,  Andrew WALGRAVE ,  Carolyn REIFSNYDER ,  Jonathan C. FEARNOW ,  Douglas GREINER ,  Chris GERGLEY

IPC: G01N35/08 ,  B01D17/02

CPC classification number: G01N35/08 ,  B01D17/0214

Abstract: Systems, including methods and apparatus, for analyzing partitioned samples, such as droplets, using recirculated fluid. The systems may be used to analyze a plurality of partitioned samples, with fluid used with initial samples reused with later samples. This reuse, or recirculation, may reduce the amount of fluid required for performing multiple analyses, with concomitant reductions in costs. Moreover, in some embodiments, it may simplify operation by increasing the number of analyses that may be performed before fluid must be replenished or replaced.

公开(公告)号：US20230070234A1

公开(公告)日：2023-03-09

申请号：US17986873

申请日：2022-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. STUMBO ,  George CARMAN ,  Steve HOBBS ,  Anthony J. MAKAREWICZ, JR. ,  Dmitri SIMONIAN ,  David GLADE ,  Joshua OEN ,  Denis PRISTINSKI ,  John DZENITIS

IPC: C12Q1/686 ,  B01L3/00 ,  G01N15/14 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6851 ,  B01F23/41

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US20220097068A1

公开(公告)日：2022-03-31

申请号：US17549163

申请日：2021-12-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas Henry CAULEY, III ,  John DZENITIS ,  Steve HOBBS ,  Stefano SCHIAFFINO

IPC: B01L3/00 ,  G01N1/38 ,  B01F3/08 ,  B01F13/10

Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.

公开(公告)号：US20190002956A1

公开(公告)日：2019-01-03

申请号：US16022500

申请日：2018-06-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. STUMBO ,  George CARMAN ,  Steve HOBBS ,  Anthony J. MAKAREWICZ, JR. ,  Dmitri SIMONIAN ,  David GLADE ,  Joshua OEN ,  Denis PRISTINSKI ,  John DZENITIS

IPC: C12Q1/686 ,  B01L3/00 ,  G01N15/14 ,  B01F3/08 ,  C12Q1/6848 ,  C12Q1/6844

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.