公开(公告)号：US20240344017A1

公开(公告)日：2024-10-17

申请号：US18444673

申请日：2024-02-17

申请人： S2 Genomics, Inc.

发明人： Stevan Bogdan Jovanovich ,  Kaiwan Chear ,  Bruce Leisz ,  David Eberhart ,  John Bashkin

IPC分类号： C12M1/33 ,  C12M1/00 ,  C12M1/34 ,  C12N1/06 ,  G01N1/28 ,  G01N1/31 ,  G01N1/40

CPC分类号： C12M45/02 ,  C12M41/12 ,  C12M45/06 ,  C12M45/09 ,  C12M45/20 ,  C12N1/066 ,  G01N1/286 ,  G01N1/31 ,  G01N1/4077 ,  G01N2001/4088

摘要： A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.

公开(公告)号：US12077784B2

公开(公告)日：2024-09-03

申请号：US18168396

申请日：2023-02-13

申请人： Life Technologies Corporation

发明人： Xin Yu ,  Xavier de Mollerat du Jeu ,  Chao Yan Lin ,  Jian Liu ,  Jonathan Zmuda

IPC分类号： C12N1/06 ,  C12N1/22 ,  C12N7/00 ,  C12N15/10 ,  C12N15/86

CPC分类号： C12N7/00 ,  C12N1/06 ,  C12N15/86 ,  C12N2750/14041 ,  C12N2750/14152

摘要： The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.

公开(公告)号：US12048317B2

公开(公告)日：2024-07-30

申请号：US17043863

申请日：2018-10-22

申请人： South China University of Technology

发明人： Mingjun Zhu ,  Li Chen

IPC分类号： A23L27/10 ,  A23L27/00 ,  A23L29/212 ,  A23L29/281 ,  A23L31/15 ,  A23L33/145 ,  A23P10/30 ,  A61K9/48 ,  A61K9/50 ,  A61K31/122 ,  C12N1/06

CPC分类号： A23L27/10 ,  A23L27/72 ,  A23L29/212 ,  A23L29/284 ,  A23L31/15 ,  A23L33/145 ,  A23P10/30 ,  A61K9/4833 ,  A61K9/5036 ,  A61K31/122 ,  C12N1/063 ,  A23V2002/00

摘要： The present invention belongs to the technical field of condiment preparation, and specifically relates to an astaxanthin-containing condiment and a preparation method therefor. In this method, first a low-speed bead milling method is used to break the cell wall of the yeast, such that astaxanthin is directly emulsified in water, and the concentration of astaxanthin can reach 1043.17 mg/L, avoiding the use of emulsifiers and organic solvents; then a small amount of Angel yeast compound enzyme is added for enzymolysis, with the yield of amino nitrogen as high as 3.51% to 3.65% and the yield of solid matter as high as 47.18% to 49.22%; and finally the gelatinized porous starch solution and gelatin are sequentially added, with the encapsulation rate and drug-loading rate of the obtained astaxanthin-containing microcapsule powder being 75.62% to 88.5% and 1.55-10.42 mg/g, respectively. The microcapsule powder has bright color, and high astaxanthin stability and water solubility. The preparation method has the advantages of short time and mild conditions, which can reduce the industrial application cost of Phaffia rhodozyma.

公开(公告)号：US20240228560A1

公开(公告)日：2024-07-11

申请号：US18616877

申请日：2024-03-26

申请人： Amprion, Inc.

发明人： Luis Concha ,  Carly Farris ,  Bret Holguin ,  Yihua Ma

IPC分类号： C07K14/47 ,  B01L3/00 ,  C07K1/34 ,  C12N1/06 ,  C12N15/70

CPC分类号： C07K14/47 ,  B01L3/5027 ,  C07K1/34 ,  C12N1/06 ,  C12N15/70

摘要： An expression vector is provided for production of human alpha-synuclein (αS) protein or a conservative variant thereof that exhibits a decreased tendency to self-aggregate in an αS seed amplification assay (SAA). The expression vector comprises a nucleic acid sequence coding for human αS protein or a conservative variant, the nucleic acid sequence comprising codons that have been optimized to produce human αS protein or a conservative variant when expressed by a host cell such as E. Coli. The codons have been optimized to avoid amino acid misincorporation in the expressed protein. Methods for purification of the expressed protein are also provided.

公开(公告)号：US11984197B2

公开(公告)日：2024-05-14

申请号：US16892093

申请日：2020-06-03

申请人： Trace Genomics, Inc.

发明人： Ariel David Zajdband ,  Wyatt Hugh Hartman ,  David Curtis Stone ,  Rosaria Chiang

IPC分类号： C12Q1/6869 ,  C12N1/06 ,  C12Q1/06 ,  G01N33/24 ,  G16B30/00 ,  G16B35/20

CPC分类号： G16B30/00 ,  C12N1/06 ,  C12Q1/06 ,  C12Q1/6869 ,  G01N33/24 ,  G16B35/20 ,  G01N2033/243

摘要： In various embodiments of an analytics system, a spike-in including synthetic constructs of nucleic acid is added to soil samples for processing. The analytics system determines sequence reads classified to a microbe in the soil sample. The analytics system determines one or more measures of soil texture of the soil sample, for example, indicating percentages of sand, silt, and clay. The analytics system determines a measure of the microbe as a function of at least the classified sequence reads, the one or more measures of soil texture, and a mass of the spike-in. The analytics system can transmit the measure of the microbe to a client device for display on a user interface. A field where the soil sample was obtained can be treated according to the measure of the microbe.

公开(公告)号：US20240150799A1

公开(公告)日：2024-05-09

申请号：US18402122

申请日：2024-01-02

申请人： Algae Consultant LLC

发明人： Rebekah Powell

IPC分类号： C12P7/64 ,  B01D15/36 ,  B01D53/84 ,  C02F1/42 ,  C08G63/16 ,  C12M1/00 ,  C12M1/107 ,  C12M1/12 ,  C12N1/06 ,  C12N1/12 ,  C12N1/20

CPC分类号： C12P7/64 ,  B01D15/363 ,  B01D53/84 ,  C02F1/42 ,  C08G63/16 ,  C12M21/02 ,  C12M23/06 ,  C12M23/22 ,  C12M23/36 ,  C12M31/10 ,  C12M47/10 ,  C12N1/06 ,  C12N1/12 ,  C12N1/20 ,  C02F2001/422

摘要： The present disclosure describes a manufacturing apparatus to use algae as a renewable green factory for producing biodegradable bioplastic. One or more embodiments include separation of a cultivated microalgae biomass from water before use in the wet or dried state. The lipids and proteins are extracted from the biomass which leaves starch and algae precursors in the remaining material from the microalgae cells. The starch includes amylose, amylopectin, monosaccharides kinases and cyclobutadiene and is hydrolyzed into a syrup containing oligosaccharides and polysaccharides. In some cases, the syrup is used as an ingredient in a medium containing nutrient for bacterial fermentation of plastics.

公开(公告)号：US20240110172A1

公开(公告)日：2024-04-04

申请号：US18338255

申请日：2023-06-20

申请人： Immedica Pharma AB

发明人： Scott W. ROWLINSON

IPC分类号： C12N9/78 ,  C07K1/18 ,  C07K1/36 ,  C12N1/06

CPC分类号： C12N9/78 ,  C07K1/18 ,  C07K1/36 ,  C12N1/06 ,  C12Y305/03001

摘要： Described are methods for producing recombinant Arginase, such as PEGylated, cobalt-substituted recombinant human Arginase 1. Also described are pharmaceutical compositions comprising such recombinant Arginase, as well as methods of treatment and uses of such recombinant Arginase.

公开(公告)号：US20240052357A1

公开(公告)日：2024-02-15

申请号：US17766307

申请日：2020-10-02

申请人： North Carolina State University

发明人： Nathan Crook ,  Ibrahim Al'Abri

IPC分类号： C12N15/70 ,  C12N15/01 ,  C12N1/06

CPC分类号： C12N15/70 ,  C12N15/01 ,  C12N1/06

摘要： A method for directed evolution in a microbe, the method comprising: introducing into a first host cell a propagation deficient phage genome and a vector comprising a target gene sequence to be mutated and a phage propagation component responsive to induce lysis of the first host cell and to provide for propagation of the phage genome; exposing the first host cell to a mutagenesis agent; inducing lysis of the first host cell and phage propagation to produce a lysate comprising phage particles comprising the target gene sequence; and infecting a second host cell with the lysate. Systems and kits for practicing the method are also disclosed.

公开(公告)号：US20240043792A1

公开(公告)日：2024-02-08

申请号：US18490773

申请日：2023-10-20

申请人： CHONGQING UNIVERSITY OF TECHNOLOGY

发明人： XING ZHOU ,  XUEMEI LI ,  HANYI ZHANG ,  QING ZHANG

IPC分类号： C12N5/00 ,  C12N1/06 ,  C12N5/0793

CPC分类号： C12N5/0006 ,  C12N1/066 ,  C12N5/0619

摘要： The invention provides engineered mitochondria and a preparation method thereof, and relates to the technical field of mitochondria. The engineered mitochondria are formed by attaching exogenous cell membranes to outer membranes of exogenous mitochondria. The preparation method comprises the following steps: S1: extracting exogenous cell membranes from cells; S2: separating and extracting exogenous mitochondria from cells or tissue; and S3: mixing the separated and extracted exogenous mitochondria with the exogenous cell membranes in a specific ratio, thereby attaching the exogenous cell membranes to the outer membranes of the exogenous mitochondria to obtain the engineered mitochondria. The invention enables the production of engineered mitochondria with enhanced biological activity, exhibiting improved therapeutic effects on mitochondrial dysfunction-related disorders.

公开(公告)号：US20240035067A1

公开(公告)日：2024-02-01

申请号：US18349684

申请日：2023-07-10

申请人： AKONNI BIOSYSTEMS, INC.

发明人： Christopher G. COONEY ,  Rebecca HOLMBERG ,  Phillip BELGRADER ,  Peter Qiang QU

IPC分类号： C12Q1/6806 ,  G01N35/10 ,  B01L3/00 ,  B01L9/06 ,  G01N35/00 ,  C12N1/06 ,  C12N13/00 ,  C12Q1/689

CPC分类号： C12Q1/6806 ,  G01N35/1002 ,  B01L3/5082 ,  B01L3/52 ,  B01L9/06 ,  G01N35/0099 ,  C12N1/066 ,  C12N13/00 ,  C12Q1/689 ,  B01L2300/0832 ,  B01L2300/0609

摘要： An integrated sample purification system includes a housing, a sample container rack, a filter holder, and a cylindrical magnet. The sample container rack and the filter device holder are disposed in the housing. The sample container rack is configured to hold one or more sample containers, the filter device holder is configured to hold one or more filter devices. The cylindrical magnet is adjacent to and external to the sample container rack, and is rotated about a central, longitudinal axis of the magnet by an electric motor disposed in the housing to lyse cells. Molecules of interest in the lysed cells are purified using filters that bind specifically to the molecules of interest. The system is readily amenable to automation and rapid purification and analysis of molecules of interest, such as nucleic acids and proteins.