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    SYSTEMS, METHODS AND COMPOSITIONS FOR OPTICAL STIMULATION OF TARGET CELLS
    3.
    发明申请
    SYSTEMS, METHODS AND COMPOSITIONS FOR OPTICAL STIMULATION OF TARGET CELLS 审中-公开

    公开(公告)号：US20190224493A1

    公开(公告)日：2019-07-25

    申请号：US16261388

    申请日：2019-01-29

    申请人： The Board of Trustees of the Leland Stanford Junior University

    发明人： Karl Deisseroth ,  Feng Zhang ,  Viviana Gradinaru

    IPC分类号： A61N5/06 ,  C12N13/00 ,  A01K67/033 ,  C07K14/00 ,  A61K48/00 ,  C07K14/195 ,  C07K14/215 ,  C12N15/86

    摘要： Stimulation of target cells using light, e.g., in vivo or in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated NpHR-based molecule comprising a nucleic acid sequence that codes for light-activated NpHR-based molecule and a promoter. Either a high expression of the molecule manifests a toxicity level that is less than about 75%, or the light-activated NpHR-based proteins are expressed using at least two NpHR-based molecular variants. Each of the variants characterized in being useful for expressing a light-activated NpHR-based molecule that responds to light by producing an inhibitory current to dissuade depolarization of the neuron. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion pumps or for controlling inhibitory currents in a cell (e.g., in in vivo and in vitro environments).

    BACTERIORHODOPSIN FUSION MEMBRANE PROTEIN EXPRESSION SYSTEM
    6.
    发明申请
    BACTERIORHODOPSIN FUSION MEMBRANE PROTEIN EXPRESSION SYSTEM 有权
    BACTERIORHODOPSIN融合膜蛋白表达系统

    公开(公告)号：US20140099667A1

    公开(公告)日：2014-04-10

    申请号：US14041493

    申请日：2013-09-30

    申请人： Academia Sinica

    发明人： Andrew H.-J. WANG ,  Min-Feng` Hsu ,  Chii-Shen Yang ,  Hsu-Yuang Fu

    IPC分类号： C07K14/215

    CPC分类号： C07K14/215 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/50

    摘要： An expression vector is disclosed, which comprises: a) a polynucleotide sequence encoding a bacteriorhodopsin or a mutant bacteriorhodopsin; b) a multiple cloning site; c) a T7 promoter, d) a polyhistidine tag; e) a first protease cleavage site; f) optionally a second protease cleavage site; and g) optionally a linker; wherein the mutant bacteriorhodopsin comprises the residue corresponding to Asn94 of SEQ ID NO: 1. Also disclosed is a fusion membrane protein expression system, which comprises: a) a polynucleotide sequence encoding a mutant Haloarcula marismortui bacteriorhodopsin/D94N (HmBRI/D94N) or a Haloquadratum walsbyi bacteriorhodopsin (HwBR); b) a target membrane protein; and c) a T7 promoter, operably linked to the mutant HmBRI/D94N or HwBR and the target membrane protein. Host cells comprising the expression vector or the fusion membrane protein expression system and methods of using the same are also disclosed.

    摘要翻译： 公开了一种表达载体，其包括：a）编码细菌视紫红质或突变型细菌视紫红质的多核苷酸序列; b）多克隆位点; c）T7启动子，d）多组氨酸标签; e）第一个蛋白酶切割位点; f）任选的第二蛋白酶切割位点; 和g）任选的接头; 其中所述突变型细菌视紫红质包含对应于SEQ ID NO：1的Asn94的残基。还公开了融合膜蛋白表达系统，其包含：a）编码突变型Haloarcula marismortui细菌视紫红质/ D94N（HmBRI / D94N）或 Haloquadratum walsbyi细菌视紫红质（HwBR）; b）靶膜蛋白; 和c）与突变体HmBRI / D94N或HwBR和靶膜蛋白可操作地连接的T7启动子。 还公开了包含表达载体或融合膜蛋白表达系统的宿主细胞及其使用方法。

    Transmembrane polypeptide and methods of use
    7.
    发明授权
    Transmembrane polypeptide and methods of use 失效
    跨膜多肽及其使用方法

    公开(公告)号：US5739273A

    公开(公告)日：1998-04-14

    申请号：US303651

    申请日：1994-09-09

    申请人： Donald M. Engelman ,  John F. Hunt

    发明人： Donald M. Engelman ,  John F. Hunt

    IPC分类号： C07K14/215 ,  A61K38/00 ,  A61K38/02 ,  C07K5/00 ,  C07K7/00

    CPC分类号： C07K14/215

    摘要： A water-soluble polypeptide having the characteristics of the bacteriorhodopsin C helix such that it is largely hydrophobic and spontaneously inserts into a biological membrane as an alpha-helix. The polypeptide is administered as an aqueous solution or is contained within the lipid bilayer of a liposome. The polypeptide is modified so that it will interfere with the helix--helix interactions within the membranes of adverse cells or is coupled to a therapeutic substance, such as a protein or pharmaceutical compound, for transmission thereof through the membrane and into the cell.

    摘要翻译： 具有细菌视紫红质C螺旋的特征的水溶性多肽，使得其大部分是疏水性的并且自发地插入生物膜作为α-螺旋。 多肽作为水溶液施用或者包含在脂质体的脂质双层内。 修饰多肽使得它将干扰不良细胞的膜内的螺旋 - 螺旋相互作用，或者与治疗物质例如蛋白质或药物化合物偶联，以将其透过膜并进入细胞。