公开(公告)号：US06531132B1

公开(公告)日：2003-03-11

申请号：US09630611

申请日：2000-08-02

申请人： Jean-Jacques Paufique

发明人： Jean-Jacques Paufique

IPC分类号： A61K3500

CPC分类号： A61Q19/08 ,  A61K8/99 ,  C12N1/063 ,  Y10S514/844 ,  Y10S514/847

摘要： A process for extracting an active principle from yeast whereby the active principle is used to retard the degradation of the dermo-epidermal junction to improve the surface condition of the skin. The process includes forming a mixture of solubilized ground yeast in a basic medium, hydrolyzing the proteins of the mixture in a basic medium, agitating the mixture for 8 to 52 hours, filtering the mixture to separate the soluble and insoluble phases, washing with water so as to purify the active ingredient, concentrating and filtering to increase an active oligosaccharide fraction, and sterile filtering the active fraction. The active fraction is characterized by a quantity of dry material between 10 and 200 g/l, a quantity of total sugar between 10 and 100 g/l, a weight ratio of sugar/protein, and a pH between 4 and 10.

摘要翻译： 从酵母提取活性成分的方法，由此使用活性成分来延缓皮肤 - 表皮连接处的降解以改善皮肤表面状况。 该方法包括在碱性介质中形成溶解的酵母的混合物，在碱性介质中水解混合物的蛋白质，搅拌混合物8至52小时，过滤混合物以分离可溶和不溶相，用水洗涤 为了纯化活性成分，浓缩和过滤以增加活性寡糖部分，并对活性级分进行无菌过滤。 活性级分的特征在于干物质的量为10至200g / l，总糖的量为10至100g / l，糖/蛋白质的重量比，以及4至10的pH。

公开(公告)号：US06399060B1

公开(公告)日：2002-06-04

申请号：US09696819

申请日：2000-10-25

申请人： Bryan Hiromoto

发明人： Bryan Hiromoto

IPC分类号： A61K3500

CPC分类号： A01N63/04 ,  Y10S435/822 ,  Y10S435/911

摘要： A pesticide composition that includes a metabolite from a fungus selected from homeocarpic basidiomycetes and homeocarpic ascomycetes is described. In one embodiment, the fungus from which the metabolite is derived is one grown under conditions effective to substantially suppress fruiting of the fungus. More particular the pesticide compositions are provided comprising a fungal metabolite derived from a fungus selected from the group Ganoderma spp., Lactiporus spp., Lentinus spp., Morchella spp., and Pleurotus spp. The pesticide composition may be produced by culturing the fungus on a medium containing vegetable oil and a surfactant. Alternatively a filtrate from a culture of the fungus is added to the medium containing vegetable oil and a surfactant, and mixed until pesticidal activity develops.

摘要翻译： 描述了一种农药组合物，其包括选自家蚕坚果菌纲和家蚕生长型子囊菌的真菌的代谢物。 在一个实施方案中，衍生代谢物的真菌是在有效地基本上抑制真菌结实的条件下生长的。 更具体地，提供了农药组合物，其包含衍生自选自灵芝属（Ganoderma spp。），乳杆菌属（Lactiporus spp。），香菇属（Lentinus spp。），莫氏菌（Morchella spp。）和侧耳属（Pleurotus spp））的真菌的真菌代谢物。 可以通过在含有植物油和表面活性剂的培养基上培养真菌来制备农药组合物。 或者，将来自真菌培养物的滤液加入到含有植物油和表面活性剂的培养基中，并混合至杀虫活性发展。

公开(公告)号：US06315992B1

公开(公告)日：2001-11-13

申请号：US09345415

申请日：1999-06-30

申请人： Moon Jong Noh ,  Kyoung Ae Kang ,  Kwan Hee Lee

发明人： Moon Jong Noh ,  Kyoung Ae Kang ,  Kwan Hee Lee

IPC分类号： A61K3500

CPC分类号： A61K38/1841 ,  A61K35/12 ,  A61K48/00 ,  C07K14/495

摘要： The subject invention is related to a cell-mediated gene therapy treatment for orthopedic disease using a member belonging to the transforming growth factor-&bgr; (TGF-&bgr;) superfamily. TGF-&bgr; gene therapy as a new treatment method for degenerative arthritis is demonstrated. After transfection of TGF-&bgr; cDNA expression vectors into fibroblasts (NIH 3T3-TGF-&bgr;1), the cells were injected into rabbit achilles tendon and knee joints with artificially-made cartilage defects. Intratendinous injections were performed to determine the optimal concentration for in vivo expression. Partially defected cartilage model was made to simulate degenerative arthritis of the knee joint. The partial cartilage defect treated with the cell-mediated gene therapy procedure was covered by newly formed hyaline cartilage which indicates that the cells survived and stimulated matrix formation in this area. Completely denuded cartilage areas were covered by fibrous collagen.

摘要翻译： 本发明涉及使用属于转化生长因子-β（TGF-β）超家族的成员的骨科疾病的细胞介导的基因治疗治疗。 证实了TGF-β基因治疗作为退行性关节炎的新治疗方法。 将TGF-βcDNA表达载体转染到成纤维细胞（NIH 3T3-TGF-β1）后，将细胞注射到兔子跟腱和膝关节中，人工制造软骨缺损。 进行Intratendinous注射以确定体内表达的最佳浓度。 进行部分缺陷软骨模型，以模拟膝关节退行性关节炎。 用细胞介导的基因治疗程序处理的部分软骨缺损被新形成的透明软骨覆盖，表明细胞存活并刺激了该区域的基质形成。 完全剥落的软骨区域被纤维胶原覆盖。

公开(公告)号：US06270811B1

公开(公告)日：2001-08-07

申请号：US09308250

申请日：1999-05-25

申请人： Alexandra Fregonese

发明人： Alexandra Fregonese

IPC分类号： A61K3500

CPC分类号： A61K8/361 ,  A61K8/365 ,  A61K8/37 ,  A61K8/4953 ,  A61K8/65 ,  A61K8/922 ,  A61K8/99 ,  A61K2800/85 ,  A61Q3/00 ,  A61Q5/00 ,  A61Q7/00 ,  A61Q19/00 ,  A61Q19/02 ,  A61Q19/08 ,  Y10S514/886 ,  Y10S514/887

摘要： The invention concerns a cosmetic or pharmaceutical composition. This composition is characterised in that it contains a microbial culture compatible in cosmetics or in pharmacy or in therapy, in particular a culture of micro-organisms selected among the acetic or lactic bacteria, freeze-dried yeast, suspensions of micro-organisms, fermented organic substances, for example fermented pollen, or an inactivated culture of bacteria of the genus bifidobacterium or of bacteria related to this genus mixed with an essential oil and an acid preferably an organic acid, such as citric acid. This composition has an activity for regenerating the skin, in particular anti-wrinkle properties, for erasing old scars, for repairing burnt tissues, for promoting skin healing, for eliminating pigmentary spots and also for promoting growth or fresh growth of superficial body growth including nails, hair or hairs.

摘要翻译： 本发明涉及一种化妆品或药物组合物。 该组合物的特征在于其包含在化妆品或药学或治疗中相容的微生物培养物，特别是选自乙酸或乳酸菌的微生物培养物，冷冻干燥酵母，微生物悬浮液，发酵有机物 物质，例如发酵花粉，或双歧杆菌属的细菌的灭活培养物或与本品相关的细菌与精油和酸优选有机酸如柠檬酸混合。 该组合物具有用于再生皮肤的功能，特别是抗皱纹性能，用于消除旧的疤痕，修复烧伤的组织，促进皮肤愈合，消除色素斑点，以及促进包括指甲在内的表面体生长的生长或新生长 ，头发或头发。

公开(公告)号：US06265387B1

公开(公告)日：2001-07-24

申请号：US08975573

申请日：1997-11-21

申请人： Jon A. Wolff ,  Vladimir Budker ,  Stuart J. Knechtle

发明人： Jon A. Wolff ,  Vladimir Budker ,  Stuart J. Knechtle

IPC分类号： A61K3500

CPC分类号： A61K48/00 ,  A61K48/0008

摘要： A process for delivering a polynucleotide to a parenchymal cell in a mammal by injecting the polynucleotide into a blood vessel connected to the parenchymal cell in tissue or organ of the mammal such that the polynucleotide is transfected into the parenchymal cell and functionally expressed to therapeutic levels.

摘要翻译： 将多核苷酸通过将多核苷酸注射到与哺乳动物的组织或器官中的实质细胞连接的血管中将多核苷酸递送至实质细胞的方法，使得多核苷酸被转染到实质细胞中并在功能上表达为治疗水平。

公开(公告)号：US06733751B2

公开(公告)日：2004-05-11

申请号：US10384840

申请日：2003-03-10

申请人： Sean Farmer

发明人： Sean Farmer

IPC分类号： A61K3500

CPC分类号： A61K31/496 ,  A61K9/0014 ,  A61K9/0034 ,  A61K31/63 ,  A61K31/7034 ,  A61K35/74 ,  A61K35/742 ,  A61K35/744 ,  A61K35/745 ,  A61K35/747 ,  A61K47/44 ,  Y02A50/473 ,  Y10S514/887 ,  A61K2300/00

摘要： An animal-derived lipid is disclosed that is useful as a carrying agent for anti-microbial formulations. Pharmaceutical and other preparations including Emu Oil are also described as profoundly useful components in anti-bacterial, anti-fungal, and anti-viral treatments. This lipid material is extracted from the Emu (Dromais Novae-Hollandiae), an indigenous bird of Australia and New Zealand. The present invention also discloses therapeutic compositions comprising Emu Oil in combination with an extracellular product of Bacillus coagulans or Pseudomonas lindbergii strain, comprising a supernatant or filtrate of said culture suitable for topical application to the skin or mucosal membranes of a mammal, which are utilized to inhibit the growth of bacterium, yeast, fungi, virus, and combinations thereof. Additionally, the aforementioned therapeutic composition may also include an anti-microbial, anti-mycotic, and/or anti-viral agent. The present invention also discloses methods of treatment and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, by topical application of therapeutic compositions comprising Emu Oil in combination with an extracellular product of Bacillus coagulans or Pseudomonas lindbergii strain suitable for topical application to the skin or mucosal membranes of a mammal. Similarly, the aforementioned method may also employ a therapeutic composition additionally containing an anti-microbial, anti-mycotic, and/or anti-viral agent.

摘要翻译： 公开了可用作抗微生物制剂的载体的动物来源的脂质。 包括Em u油在内的药物和其他制剂也被描述为抗菌，抗真菌和抗病毒治疗中非常有用的成分。 这种脂质物质是从澳大利亚和新西兰的一只土着鸟的Emu（Dromais Novae-Hollandiae）中提取出来的。 本发明还公开了包含Em u油与凝结芽孢杆菌或绿脓假单胞菌菌株的细胞外产物组合的治疗组合物，其包含适于局部施用于哺乳动物皮肤或粘膜的所述培养物的上清液或滤液，其用于 抑制细菌，酵母，真菌，病毒及其组合的生长。 另外，上述治疗组合物还可以包括抗微生物，抗真菌和/或抗病毒剂。 本发明还公开了通过局部应用包含Em u油与凝结芽孢杆菌或绿脓假单胞菌菌株的细胞外产物组合的治疗组合物来抑制细菌，酵母，真菌，病毒及其组合的生长的治疗方法和治疗系统 适合局部应用于哺乳动物的皮肤或粘膜。 类似地，上述方法还可以使用另外含有抗微生物，抗真菌和/或抗病毒剂的治疗组合物。

公开(公告)号：US06602499B1

公开(公告)日：2003-08-05

申请号：US09302952

申请日：1999-04-30

申请人： E. Antonio Chiocca ,  Xandra O. Breakefield

发明人： E. Antonio Chiocca ,  Xandra O. Breakefield

IPC分类号： A61K3500

CPC分类号： B82Y5/00 ,  A61K31/675 ,  A61K35/763 ,  A61K38/415 ,  A61K38/44 ,  A61K45/06 ,  A61K47/6891 ,  A61K48/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/16332 ,  C12N2710/16632 ,  C12N2710/16643 ,  C12Y114/14 ,  C12Y117/04001 ,  A61K2300/00

摘要： The present invention relates to viral mutants and methods of using these viral mutants for selectively killing neoplastic cells. The viral mutants of the invention are capable of selectively killing neoplastic cells by a combination of viral mediated oncolysis and anti-cancer (“suicide”) gene therapy.

摘要翻译： 本发明涉及病毒突变体和使用这些病毒突变体选择性杀死肿瘤细胞的方法。 本发明的病毒突变体能够通过病毒介导的溶瘤和抗癌（“自杀”）基因治疗的组合选择性地杀死肿瘤细胞。

公开(公告)号：US06544525B1

公开(公告)日：2003-04-08

申请号：US10071355

申请日：2002-02-07

申请人： Inna Yegorova

发明人： Inna Yegorova

IPC分类号： A61K3500

CPC分类号： A61K36/06 ,  A61K31/122 ,  A61K31/355 ,  A61K36/062 ,  A61K36/88 ,  Y10S514/824 ,  A61K2300/00

摘要： This invention provides compositions and methods related to the administration of red yeast rice, coenzyme Q10, and chromium, with or without inositol hexanicotinate, selenium, and mixed tocopherols to reduce or control blood cholesterol, triglycerides, low density lipoproteins, or increasing or controlling high density lipoproteins in a mammal, to reduce arterial plaque build-up, atherosclerosis, in a mammal which may be associated with cardiovascular, cerebrovascular, peripheral vascular, or intestinal vascular disorders.

公开(公告)号：US06270776B1

公开(公告)日：2001-08-07

申请号：US08454075

申请日：1995-05-30

申请人： Barry R. Bloom ,  Ronald W. Davis ,  William R. Jacobs, Jr. ,  Richard A. Young ,  Robert N. Husson

发明人： Barry R. Bloom ,  Ronald W. Davis ,  William R. Jacobs, Jr. ,  Richard A. Young ,  Robert N. Husson

IPC分类号： A61K3500

CPC分类号： A61K39/04 ,  A61K39/00 ,  C07K14/005 ,  C07K14/35 ,  C12N15/74 ,  C12N15/87 ,  C12N2795/10022 ,  C12R1/32 ,  Y02A50/412 ,  Y02A50/476

摘要： Recombinant mycobacterial vaccine vehicles capable of expressing DNA of interest which encodes at least one protein antigen for at least one pathogen against which an immune response is desired and which can be incorporated into the mycobacteria or stably integrated into the mycobacterial genome. The vaccine vehicles are useful for administration to mammalian hosts for purposes of immunization. A recombinant vector which replicates in E. coli but not in mycobacteria is also disclosed. The recombinant vector includes 1) a mycobacterial gene or portions thereof, necessary for recombination with homologous sequences in the genome of mycobacteria transformed with the recombinant plasmid; 2) all or a portion of a gene which encodes a polypeptide or protein whose expression is desired in mycobacteria transformed with the recombinant plasmid; 3) DNA sequences necessary for replication and selection in E. coli; and 4) DNA sequences necessary for selection in mycobacteria (e.g., drug resistance). The present invention also relates to two types of recombinant vectors useful in introducing DNA of interest into mycobacteria, where it is expressed. One type of vector is a recombinant phasmid capable of replicating as a plasmid in E. coli and of lysogenizing a mycobacterial host. The other type of vector is a recombinant plasmid which can be introduced into mycobacteria, where it is stably maintained extrachromosomally.

摘要翻译： 能够表达目的的DNA的重组分枝杆菌疫苗载体，其编码针对至少一种需要免疫应答的至少一种病原体的至少一种蛋白质抗原并且可以并入分枝杆菌中或稳定整合到分枝杆菌基因组中。 疫苗载体用于哺乳动物宿主用于免疫的目的。 还公开了在大肠杆菌中但不在分枝杆菌中复制的重组载体。 重组载体包括1）与用重组质粒转化的分枝杆菌基因组中与同源序列重组所必需的分枝杆菌基因或其部分; 2）编码在用重组质粒转化的分枝杆菌中表达所需的多肽或蛋白质的基因的全部或部分; 3）在大肠杆菌中复制和选择所需的DNA序列; 和4）在分枝杆菌中选择所需的DNA序列（例如耐药性）。 本发明还涉及可用于将感兴趣的DNA引入分枝杆菌的两种类型的重组载体，其中它被表达。 一种载体是能够在大肠杆菌中作为质粒复制并使分枝杆菌宿主溶解的重组噬菌粒。 另一种载体是可以引入分枝杆菌的重组质粒，其在染色体外稳定地保持。

公开(公告)号：US06200560B1

公开(公告)日：2001-03-13

申请号：US09470618

申请日：1999-12-22

申请人： Linda B. Couto ,  Peter C. Colosi ,  Xiaobing Qian

发明人： Linda B. Couto ,  Peter C. Colosi ,  Xiaobing Qian

IPC分类号： A61K3500

CPC分类号： C12N15/86 ,  A61K38/37 ,  A61K48/00 ,  C07K14/755 ,  C12N2750/14143 ,  C12N2799/025 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/85

摘要： The present invention provides improved viral vectors useful for the expression of genes at high levels in human cells. In particular, the present invention provides recombinant adeno-associated vectors (AAV) suitable for gene therapy. These vectors are capable of delivering nucleic acid containing constructs which result in the production of full-length therapeutic levels of biologically active Factor VIII in the recipient individual in vivo. The present invention also provides pharmaceutical compositions comprising such AAV vectors, as well as methods for making and using these constructs.

摘要翻译： 本发明提供了可用于在人细胞中高水平表达基因的改进的病毒载体。 特别地，本发明提供适用于基因治疗的重组腺相关载体（AAV）。 这些载体能够递送含有核酸的构建体，其导致在体内在受体个体中产生全长治疗水平的生物活性因子VIII。 本发明还提供包含这种AAV载体的药物组合物，以及制备和使用这些构建体的方法。