摘要:
In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.
摘要:
A novel technique is provided for automatically performing the separation and transfer of analyte, and subsequent processing. A sample separation/transfer device (100) is a device for separating analyte by way of electrophoresis, dispensing the separated analyte from a dispensing part (50a) inside a buffer tank (30), and transferring the separated analyte to a transfer membrane (1) by way of causing the transfer membrane (1) to abut the dispensing part (50a) and move, in which the device includes a liquid delivery pump (11a, 11b) that replaces the liquid filling the buffer tank (30).
摘要:
An electrode assembly for capillary electrophoresis (CE) comprises a manifold (310), a connector (305) a sheath (300), and a seal (325). A capillary tube (100) passes through the manifold, the connector, the sheath, and the seal, stopping just beyond the end of the sheath. The sheath is fillable with water (330) or another fluid that cools the capillary tube in the vicinity of the electrode, thereby preventing degradation of a sample due to heat. The sheath may be metal or plastic with a metal sleeve electrode on its exterior. The sheath is sufficiently strong to penetrate a rubber or other pierceable cap on a vial. The manifold and connector incorporate an air path (605, 312, 307) so that when the electrode is fully inserted into a vial, the contents (650) of the vial are at atmospheric pressure (or another applied pressure or vacuum).
摘要:
A first dimension electrophoresis apparatus of vertical type with a gel container 2 having a plurality of rectangular rod-shaped gel chambers is provided. The rod-type gels surrounded with non-conductive material plates are arranged in parallel, an upper open end of the gel container 2 is placed tightly into an upper buffer reservoir 4 and the lower open end thereof is placed tightly into a lower buffer reservoir 1. The gel container 2 and the main parts of the first buffer reservoir 4 and the lower buffer reservoir 1 are sunk in a cooling reservoir 5 filled with cooling liquid. The lower buffer reservoir has a panel portion 1b which is bent upward to penetrate the cooling reservoir. Outlet conductors thereof extend from each of the reservoirs and are insulated physically and electrically from the cooling liquid. As a result, the outer surface of the gel container can directly contact with the cooling liquid.
摘要:
Even if a standby time to the measurement becomes long, a sample in a sample container is prevented from deteriorating. A cooling mechanism is provided in a sample tray for holding a sample plate, or in a plate-mounting portion of a table-type auto sampler of a capillary electrophoresis apparatus, so that the sample plate is cooled during the measurement also.
摘要:
A method of electrophoresis, an electrophoresis apparatus and a capillary array in which a fluorescently labelled sample is supplied into capillaries in a capillary array constituted by a plurality of capillaries and including a sample supply portion, an electrophoresis medium supply portion and a detection portion while controlling the temperature of the capillary array through gas circulation, the sample is caused to migrate and separated in the capillaries through electrophoresis, and at the region where the capillaries are contacted or come close each other a plurality of capillaries are contacted to a solid body so as to dissipate heat generated from the capillaries to the solid body.
摘要:
In a case where the temperature of capillaries cannot be controlled rapidly in accordance with the change of the amount of heat generated from the capillaries, the phoretic speed of the sample is not controlled constant to lower the analyzing performance for the sample. This invention intends to stabilize the electrophoretic speed. The surface of a substrate on which a plurality of capillaries are arranged is in contact with a member capable of temperature control thereby controlling the temperature of the capillaries. Since a member capable of controlling the temperature and the capillaries are in direct contact with each other according to the invention, the temperature of the capillary can be controlled rapidly at a high accuracy, so that electrophoretic speed is stabilized.
摘要:
An electrophoresis device for use in separating a mixture of analytes in a fluid sample is provided, the device comprising a first dimension separation medium through which the analytes may migrate, the separation medium being carried on one face of a flexible sheet, the surface area of that face being greater than that of the region of contact between the separation medium and the sheet, wherein the first dimension separation medium is located within a first separation zone which is defined at least partly by the flexible sheet, the device further comprising a fluid chamber, separated from the first dimension separation zone by the flexible sheet, in which fluid may be retained in contact with that face of the sheet opposite to the face on which the separation medium is carried. The device may additionally comprise a second separation zone, in which a second separation medium may be located, in order to carry out a second dimension electrophoretic separation.
摘要:
A polymer-based microfluidic device and its manufacturing methods. This microfluidic device is suitable for chromatographic and electrophoretic separations in which the detection of the components in the fluid is by means of ultraviolet (UV) spectroscopy. The device further comprises at least one cooling channel adjacent the separation channel, said cooling channel conducting a cooling fluid through the device for removing heat from the the separation channel. At least one section of the separation channel has an increased cross-sectional dimension with respect to other sections of said channel to provide an increased optical path length when the device is used in an ultraviolet detection arrangement.
摘要:
Scattering on analysis in a multi-capillary electrophoresis apparatus is suppressed. The multi-capillary electrophoresis apparatus has a multi-capillary array that has a sample and a separation medium for separating a sample charged therein, and has a sampling section formed at ends of the capillaries and a detector part for acquiring information depending on the sample thus separated, a means for applying a voltage between the sampling section and the detector part, a chamber part having an air blowing mechanism and a temperature controlling mechanism, a capillary housing base provided on a leeward side of the air blowing mechanism and housing the multi-capillary array, and a first straightening plate provided between the chamber part and the capillary housing base.