公开(公告)号：US10408787B2

公开(公告)日：2019-09-10

申请号：US13976157

申请日：2011-11-30

Applicant: Motohiro Yamazaki ,  Michiru Fujioka ,  Takamichi Muramatsu ,  Takashi Gomi

Inventor： Motohiro Yamazaki ,  Michiru Fujioka ,  Takamichi Muramatsu ,  Takashi Gomi

IPC: G01N27/447 ,  G01N30/60

Abstract: In an apparatus for analyzing small number of samples, wasteful consumption of a polymer as a separation Medium is suppressed.The apparatus has a configuration provided with a plurality of capillary units each of which can be attached/detached to/from the apparatus, and performs analysis for capillaries after sealing of a polymer by attaching the capillaries by the number in accordance with the number of samples. Since analysis can be performed by using capillaries by the number identical with that of the samples to be analyzed, wasteful polymer is not used in the capillaries not used for the analysis. In addition, the polymer injection mechanism is unnecessary and the polymer is not necessary for maintenance and upon starting of analysis. An electrophoresis apparatus which is small in the size with no polymer injection mechanism, and with low running cost not wastefully consuming the polymer can be provided.

公开(公告)号：US20130292251A1

公开(公告)日：2013-11-07

申请号：US13976157

申请日：2011-11-30

Applicant: Motohiro Yamazaki ,  Michiru Fujioka ,  Takamichi Muramatsu ,  Takashi Gomi

Inventor： Motohiro Yamazaki ,  Michiru Fujioka ,  Takamichi Muramatsu ,  Takashi Gomi

IPC: G01N27/447

Abstract: In an apparatus for analyzing small number of samples, wasteful consumption of a polymer as a separation Medium is suppressed.The apparatus has a configuration provided with a plurality of capillary units each of which can be attached/detached to/from the apparatus, and performs analysis for capillaries after sealing of a polymer by attaching the capillaries by the number in accordance with the number of samples. Since analysis can be performed by using capillaries by the number identical with that of the samples to be analyzed, wasteful polymer is not used in the capillaries not used for the analysis. In addition, the polymer injection mechanism is unnecessary and the polymer is not necessary for maintenance and upon starting of analysis. An electrophoresis apparatus which is small in the size with no polymer injection mechanism, and with low running cost not wastefully consuming the polymer can be provided.

Abstract translation: 在分析少量样品的装置中，抑制作为分离介质的聚合物的浪费的消耗。 该装置具有设置有多个毛细管单元的构造，每个毛细管单元可以与该装置相连接/分离，并且在密封聚合物之后对毛细管进行分析，该毛细管通过根据样品数量附加毛细管数量 。 由于可以通过使用与要分析的样品相同数量的毛细管进行分析，所以在不用于分析的毛细管中不使用浪费的聚合物。 此外，聚合物注入机制是不必要的，并且聚合物不需要维护和分析开始时。 可以提供一种尺寸小且无聚合物注射机制，并且运行成本低，不浪费地消耗聚合物的电泳装置。

公开(公告)号：US08349161B2

公开(公告)日：2013-01-08

申请号：US13263304

申请日：2010-04-06

Applicant: Takashi Gomi ,  Ryoji Inaba ,  Taro Nakazawa ,  Takeshi Ohura ,  Mari Kotoura

Inventor： Takashi Gomi ,  Ryoji Inaba ,  Taro Nakazawa ,  Takeshi Ohura ,  Mari Kotoura

IPC: G01N27/453

CPC classification number: F04B19/006 ,  B01D57/02 ,  G01N27/44704

Abstract: Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.

Abstract translation: 不管泵的个体差异，将电泳介质填充到毛细管中，都可以去除气泡。 在容纳电泳介质的容器的内侧面和柱塞的侧面之间形成的流路之间形成有使电泳介质容易停滞的流路之一，其横截面积大于 另一个流动通道的相对侧的横截面面积。 换句话说，导致电泳介质容易停滞的流通部分以增加电泳介质的流量的方式形成。 这可以消除在泵中具有极少量的电泳介质流动的区域。

公开(公告)号：US08257569B2

公开(公告)日：2012-09-04

申请号：US12352238

申请日：2009-01-12

Applicant: Tomohiro Shoji ,  Toshiyuki Sakurai ,  Takashi Gomi ,  Ryoji Inaba

Inventor： Tomohiro Shoji ,  Toshiyuki Sakurai ,  Takashi Gomi ,  Ryoji Inaba

IPC: G01N27/453

CPC classification number: G01N27/44782

Abstract: In a capillary electrophoresis apparatus, a capillary array unit has a capillary array including capillaries having a capillary head and a detection unit, a frame for supporting the capillary array, and a load header for holding cathode ends of the capillaries. The frame has separators for separating and holding the capillaries. The capillary head, the detection unit, and the separators are disposed along one linear line. With this arrangement, there is provided the capillary array unit and the capillary electrophoresis apparatus which are arranged such that a job for mounting the capillary array can be executed easily.

Abstract translation: 在毛细管电泳装置中，毛细管阵列单元具有毛细管阵列，其包括具有毛细管头和检测单元的毛细管，用于支撑毛细管阵列的框架和用于保持毛细血管的阴极端的负载头。 框架具有用于分离和保持毛细管的分离器。 毛细管头，检测单元和分离器沿着一条线性线设置。 通过这种布置，提供了毛细管阵列单元和毛细管电泳装置，其布置成使得可以容易地执行用于安装毛细管阵列的作业。

公开(公告)号：US08702949B2

公开(公告)日：2014-04-22

申请号：US13263040

申请日：2010-04-08

Applicant: Takeshi Ohura ,  Ryoji Inaba ,  Manabu Akiba ,  Takashi Gomi ,  Mari Kotoura

Inventor： Takeshi Ohura ,  Ryoji Inaba ,  Manabu Akiba ,  Takashi Gomi ,  Mari Kotoura

IPC: G01N27/453

CPC classification number: G01N27/44743 ,  G01N27/44782

Abstract: The solution reservoir apparatus of the capillary electrophoresis apparatus securely affixes an evaporation-preventing membrane to a container when a capillary is inserted or withdrawn, without extending the cathode end of the capillary. The solution reservoir apparatus comprises a container for reserving a sample or solution, a cover having a bore through which the capillary is passed and covering the container, an evaporation-preventing membrane having a capillary hole through which the capillary is passed, and a container holder for holding the container. The evaporation-preventing membrane has a projection provided at the periphery of the capillary hole, the projection of the evaporation-preventing membrane is engaged with the bore on the cover when the evaporation-preventing membrane is positioned on the cover, and the evaporation-preventing membrane is supported by the cover.

Abstract translation: 当毛细管插入或取出时，毛细管电泳装置的溶液储存装置在不延长毛细管的阴极端的情况下，将防蒸发膜牢固地固定在容器上。 溶液储存装置包括用于储存样品或溶液的容器，具有孔的盖，毛细管穿过孔覆盖容器，具有毛细孔通过的毛细孔的防蒸发膜和容器保持架 用于拿着容器。 防蒸发膜具有设置在毛细孔周边的突起，当防蒸发膜位于盖上时，防蒸发膜的突起与盖上的孔接合，并且防蒸发膜 膜由盖支撑。

公开(公告)号：US20120031762A1

公开(公告)日：2012-02-09

申请号：US13263304

申请日：2010-04-06

Applicant: Takashi Gomi ,  Ryoji Inaba ,  Taro Nakazawa ,  Takeshi Ohura ,  Mari Kotoura

Inventor： Takashi Gomi ,  Ryoji Inaba ,  Taro Nakazawa ,  Takeshi Ohura ,  Mari Kotoura

IPC: F04B19/20 ,  G01N27/00

CPC classification number: F04B19/006 ,  B01D57/02 ,  G01N27/44704

Abstract: Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.

Abstract translation: 不管泵的个体差异，将电泳介质填充到毛细管中，都可以去除气泡。 在容纳电泳介质的容器的内侧面和柱塞的侧面之间形成的流路之间形成有引起电泳介质容易停滞的流路之一，其横截面积大于 另一个流动通道的相对侧的横截面面积。 换句话说，导致电泳介质容易停滞的流通部分以增加电泳介质的流量的方式形成。 这可以消除在泵中具有极少量的电泳介质流动的区域。

公开(公告)号：US07787120B2

公开(公告)日：2010-08-31

申请号：US12146920

申请日：2008-06-26

Applicant: Motohiro Yamazaki ,  Ryoji Inaba ,  Kosaku Toyosaki ,  Shintaro Kubo ,  Takashi Gomi ,  Katsunobu Hama ,  Kiyotoshi Mori ,  Hideyuki Akiyama

Inventor： Motohiro Yamazaki ,  Ryoji Inaba ,  Kosaku Toyosaki ,  Shintaro Kubo ,  Takashi Gomi ,  Katsunobu Hama ,  Kiyotoshi Mori ,  Hideyuki Akiyama

IPC: G01J3/10 ,  G01J3/36 ,  G01N21/33

CPC classification number: G01J3/10 ,  G02B5/283 ,  G02B27/1006 ,  G02B27/1086 ,  G02B27/145 ,  G02B27/148

Abstract: Detection sensitivity is improved by increasing the amount of light of beams that irradiate a sample cell without causing saturation of a detector with ultraviolet beams or visible beams. This spectrophotometer includes a sample cell, which stores a sample to be measured, a visible light source and an ultraviolet light source each for supplying an incident beam that enters the sample, a spectroscope, which disperses a beam that has passed through the sample, an optical detector, which detects beams dispersed from such beam (spectrum), and a dichroic element which reflects or transmits ultraviolet beams from the ultraviolet light source and which transmits or reflects visible beams from the visible light source. Optics are configured such that ultraviolet beams and visible beams that have passed through or have been reflected by the dichroic element enter the sample cell.

Abstract translation: 通过增加照射样品池的光束的光量而不会使紫外线束或可见光束的检测器饱和而提高检测灵敏度。 该分光光度计包括存储待测样品，可见光源和紫外光源的样品池，每个用于提供进入样品的入射光束，分散已经通过样品的光束的分光器， 检测从这种光束（光谱）分散的光束的光检测器，以及从紫外光源反射或透射紫外光束并从可见光源透射或反射可见光束的二向色元件。 光学构造使得已经通过或已被二色性元件反射的紫外光束和可见光束进入样品池。

公开(公告)号：US20080296161A1

公开(公告)日：2008-12-04

申请号：US12153395

申请日：2008-05-19

Applicant: Tomohiro Shoji ,  Takashi Gomi

Inventor： Tomohiro Shoji ,  Takashi Gomi

IPC: G01N27/447

CPC classification number: G01N27/44743

Abstract: To obtain a stable pressure to inject polymer into a capillary array in order to shorten the injection time and raise the processing capacity of the apparatus.Disclosed herein is an electrophoresis apparatus comprising one or more capillaries to be filled with an electrophoretic medium and a pump mechanism which can be connected to the capillaries for filling the capillaries with an electrophoretic medium. The pump mechanism comprises a plunger; a slider which is bonded to the plunger and moves together with the plunger; a spring which gives force to the slider; a cover which limits the range of displacement of the slider; and a linear motion system which is bonded to the cover and moves linearly, wherein the cover and the slider move as one body when the electrophoretic medium is taken to the plunger, and the plunger is pushed by the action of the spring when the electrophoretic medium is injected from the plunger into the capillaries.

Abstract translation: 为了获得稳定的压力以将聚合物注入到毛细管阵列中，以缩短注射时间并提高装置的处理能力。 本文公开了一种电泳装置，其包括一个或多个要填充电泳介质的毛细管和泵机构，该机构可连接到毛细管以用电泳介质填充毛细管。 泵机构包括柱塞; 滑块，其结合到柱塞并与柱塞一起移动; 向滑块施力的弹簧; 限制滑块的位移范围的盖; 以及线性运动系统，其结合到所述盖并线性移动，其中当所述电泳介质被取入所述柱塞时，所述盖和所述滑块作为一体移动，并且当所述电泳介质 从柱塞注入毛细管。

公开(公告)号：US07883613B2

公开(公告)日：2011-02-08

申请号：US11806889

申请日：2007-06-05

Applicant: Takashi Gomi ,  Ryoji Inaba ,  Motohiro Yamazaki ,  Hidenori Namba ,  Jin Matsumura ,  Hiromi Yamashita ,  Seiichi Ugai ,  Kazumichi Imai

Inventor： Takashi Gomi ,  Ryoji Inaba ,  Motohiro Yamazaki ,  Hidenori Namba ,  Jin Matsumura ,  Hiromi Yamashita ,  Seiichi Ugai ,  Kazumichi Imai

IPC: G01N27/453

CPC classification number: G01N27/44721

Abstract: The present invention provides a capillary electrophoresis apparatus in which a capillary is easily attached to and detached from a migration medium filling unit without mixing impurities into the capillary. Mixture of impurities such as dust is also prevented when a capillary negative-electrode end is brought into contact with a sample stored in a vessel. Furthermore, temperature control is efficiently performed in the capillary. In the capillary electrophoresis apparatus, the whole of capillary array can be supported by grasping a grip portion by hand. A migration medium filling mechanism includes a slide mechanism which moves a polymer block with respect to a capillary head. The capillary electrophoresis apparatus includes a vessel in which a sample and a buffer can simultaneously be stored. Temperatures of the capillary and an irradiation and detection unit are controlled by a temperature control function provided in a thermostatic device.

Abstract translation: 本发明提供了一种毛细管电泳装置，其中毛细管容易地连接到迁移介质填充单元并从迁移介质填充单元中分离而不将杂质混入毛细管中。 当毛细管负极端与存储在容器中的样品接触时，也可以防止诸如灰尘等杂质的混合物。 此外，在毛细管中有效地进行温度控制。 在毛细管电泳装置中，通过用手抓握把手部分可以支撑整个毛细管阵列。 迁移介质填充机构包括相对于毛细管头移动聚合物嵌段的滑动机构。 毛细管电泳装置包括可以同时存储样品和缓冲液的容器。 毛细管和照射和检测单元的温度由恒温装置中提供的温度控制功能控制。

公开(公告)号：US20080217177A1

公开(公告)日：2008-09-11

申请号：US12073296

申请日：2008-03-04

Applicant: Ryoji Inaba ,  Motohiro Yamazaki ,  Tomohiro Shoji ,  Takeshi Ohura ,  Takashi Gomi

Inventor： Ryoji Inaba ,  Motohiro Yamazaki ,  Tomohiro Shoji ,  Takeshi Ohura ,  Takashi Gomi

IPC: G01N27/26

CPC classification number: G01N27/447

Abstract: There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed.Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.

Abstract translation: 提供了一种毛细管电泳装置，其中激光照射光与毛细管阵列的耦合效率即使去除两个毛细管阵列的任何一个毛细管阵列也不会下降。 通过一个光学检测系统检测由一个辐射来自一侧的激发光的2μs（n是正整数）毛细管阵列的毛细管阵列的毛细管产生的发光。