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    PURIFICATION CHEMISTRIES AND FORMATS FOR SANGER DNA SEQUENCING REACTIONS ON A MICRO-FLUIDICS DEVICE
    1.
    发明申请
    PURIFICATION CHEMISTRIES AND FORMATS FOR SANGER DNA SEQUENCING REACTIONS ON A MICRO-FLUIDICS DEVICE 有权

    公开(公告)号：US20250019757A1

    公开(公告)日：2025-01-16

    申请号：US18405788

    申请日：2024-01-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Swati Goyal ,  Jonathan Wang ,  Hans Wenz ,  Peter Ma ,  Achim Karger ,  S. Jeffrey Rosner ,  Ian Walton

    IPC: C12Q1/6869 ,  B01L3/00 ,  C12Q1/6806

    Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

    Purification Chemistries and Formats for Sanger DNA Sequencing Reactions on a Micro-Fluidics Device
    3.
    发明申请
    Purification Chemistries and Formats for Sanger DNA Sequencing Reactions on a Micro-Fluidics Device 审中-公开
    净化化学品和格式的微流体装置上的Sanger DNA测序反应

    公开(公告)号：US20170051344A1

    公开(公告)日：2017-02-23

    申请号：US15113786

    申请日：2015-01-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Swati Goyal ,  Achim Karger ,  Peter Ma ,  S. Jeffrey Rosner ,  Ian Walton ,  Jonathan Wang ,  Michael Wenz

    IPC: C12Q1/68 ,  B01L3/00

    CPC classification number: C12Q1/6869 ,  B01L3/502753 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/16 ,  C12Q1/6806

    Abstract: According to various embodiments described herein, a microfiuidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfiuidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfiuidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

    Abstract translation: 根据本文描述的各种实施方案，提供了一种用于Sanger-测序反应的基于微型芯片的纯化装置和系统。 该装置和系统允许引入包含特定于测序污染物或测序方法的纯化技术的柱的测序系统，其中盒的简化纯化溶液允许样品纯化过程的自动化，净化试剂的消耗降低和一致性 通过减少采样误差和伪像进行采样。 因此，这些各种实施方案解决了对于用于CE器件的基于微流化芯片的Sanger-测序反应纯化系统的需求。 所描述的微流体芯片可以通过重组片上试剂，反应孔和工作流程步骤用作PCR芯片。

    Compositions, methods, kits and devices for molecular analysis
    4.
    发明授权
    Compositions, methods, kits and devices for molecular analysis 有权

    公开(公告)号：US12153014B2

    公开(公告)日：2024-11-26

    申请号：US18108471

    申请日：2023-02-10

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  INTEGENX INC.

    Inventor： Jen-Kuei Liu ,  Bharti Solanki ,  Hans Michael Wenz ,  Frank Mercer ,  Cevat Akin ,  Achim Karger ,  Annelise Barron ,  Scott Mack

    IPC: G01N27/447 ,  C08L33/26

    Abstract: Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
    Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.

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