Abstract:
A variable filter spectrophotometer, for use with sample and reference; has a main member, a filter unit, a drive, a detector, a light distribution system, and a clamping circuit. The main member defines first and second beam paths, which are intersected by the filter unit. The filter unit has filtering and opaque portions. The filter unit is continuously movable relative to the beam paths in a repeating cycle from a first filtering relation in which the filtering portion is interposed in the first beam path and the opaque portion completely blocks the second beam path, to a first dark relation in which both beam paths are blocked, to a second filtering relation in which the filtering portion is interposed in the second beam path and the first beam path is completely blocked, and to a second dark relation in which both beam paths are completely blocked. The filtering portion is variably transmissive along a direction of movement of the filter unit. The drive continuously moves the filter unit relative to the beam paths. The detector produces a signal responsive to light received. The light distribution system directs light separately to and from the sample and reference, to and from the beam paths, and to the detector. The clamping circuit clamps the signal produced by the detector during the filtering relations to the signal produced by the detector during the dark relations.
Abstract:
A low cost optical system which incorporates a low ultraviolet output tungsten halogen light source and solid state photodetectors and circuitry in such a way as to provide reliable fluorometric test results. The attainment of reliable results using such components is made possible by incorporating highly ultraviolet transmissive optics to maximize ultraviolet light throughput and by using solid state circuitry together with a filter wheel having both light blocking and light passing regions in a manner which fully accounts for noise and dark signals associated with solid state photodetectors.
Abstract:
Methods and assemblies are provided for evaluating plants for presence of pests. Methods may include separating pests from a plant to produce a sample of pests for analysis, illuminating the sample to produce emitted light from the sample, and comparing the emitted light from the sample to a model to discriminate pests within the sample. Assemblies may include a separating unit operable to separate pests from a plant to produce a sample comprising pests, a light source for illuminating at least part of the sample, and an imaging device adjacent the light source for receiving light from the illuminated sample and creating an image of the sample.
Abstract:
A system uses a photometer that establishes an optical channel between a light emitter and a light detector. The photometer analyses a specimen while it occupies a well in a tray. A movable platform transports the tray to the optical channel for analysis and removes the tray from the optical channel after analysis. The system calibrates the movable platform to assure proper alignment between the specimen and the photometric apparatus used to analyzed the specimen. The system also calibrates the optical channel without use of an external reference.
Abstract:
An automatic test instrument for gaging the percentage of various constituents in organic substances by comparing the reflective optical density of the subject at various wavelengths. Narrow band optical filters are connected together in the form of a rotatable paddle wheel positioned so that the filters can be individually swept through the incident light path between the specimen and wideband light source. As the filter wheel turns, the band of light passed by each filter is progressively shifted with the changing angle of the filter relative to the light path. The filter wheel configuration includes opaque vanes extending from the ends of the filters to periodically interrupt the passage of light to the specimen. Photocells are positioned to sense the level of light reflected from the specimen. The output of the photocells is sampled at predetermined times relative to the rotation of the filter wheel to yield values indicative of reflected intensity at certain wavelengths. Using these values, an electronic circuit in one embodiment calculates three optical density difference values corresponding to moisture, protein and oil content of the specimen. The difference values are automatically inserted in three linear equations which are solved to obtain readings representing the true percentages of oil, water and protein contained in the specimen. Each time a new specimen is loaded for testing, the instrument is automatically calibrated against a standard sample, preferably Teflon (Trademark). The output of the photocells is amplified in a special circuit which subtracts the level of dark period current from the output when the photocells are illuminated.
Abstract:
Methods and assemblies are provided for evaluating plants for presence of pests. Methods may include separating pests from a plant to produce a sample of pests for analysis, illuminating the sample to produce emitted light from the sample, and comparing the emitted light from the sample to a model to discriminate pests within the sample. Assemblies may include a separating unit operable to separate pests from a plant to produce a sample comprising pests, a light source for illuminating at least part of the sample, and an imaging device adjacent the light source for receiving light from the illuminated sample and creating an image of the sample.
Abstract:
A spectrophotometer is described, comprising a single light source, a single detector, optics for dually and alternatively reading a sample and a reference, and only one moving part. That moving part is a chopper containing multiple pass-through apertures, each filled with a unique bandpass filter to select wavelengths to specifically illuminate the sample or reference. To inform the spectrophotometer whether and when it is reading the sample or the reference, trigger means are provided in at least twice the number of the pass-through apertures.
Abstract:
An improved grain quality analyzer, for analyzing the percentage concentration of various constituents, e.g. protein and water, in a grain sample, photo-optically measures the change in the optical density of the sample, .DELTA. OD, in a range of characteristic wavelengths and, for protein, in a range of neutral wavelengths and uses these measured values to compute the percentage concentration of the constituents.A grain sample is irradiated with light, the wavelength of which sweeps across the infrared light-spectrum including a range of wavelengths termed characteristic wavelengths, and, for protein, a range of wavelengths termed neutral wavelengths. The characteristic wavelengths are those wavelengths at which the optical characteristics of the irradiated sample, that is, the optical density, reflectivity, transmissivity, and/or absorption, are known to vary as a function of the concentration of the measured constituent, and, for the protein measurement, the neutral wavelengths are those wavelengths at which the optical characteristics are substantially independent of the concentration of the protein.In the preferred embodiment, photo-optical sensors measure the light reflected from the sample and provide output signals indicative of the optical density of the sample as a function of the wavelength of the irradiating light. Control and computing means sample the sensor output at spaced apart points in the range of characteristic wavelengths and in the range of neutral wavelengths to provide signals representative of the optical characteristics of the sample. Computing circuitry computes the percentage concentration of water and, for protein, computes the percentage concentration using an algorithm in accordance with the present invention which eliminates the inaccuracies introduced by light scattering from the sample.
Abstract:
An automatic test instrument for gaging the percentage of various constituents in organic substances by comparing the reflective optical density of the subject at various wavelengths. Narrow band optical filters are connected together in the form of a rotatable paddle wheel positioned so that the filters can be individually swept through the incident light path between the specimen and wideband light source. As the filter wheel turns, the band of light passed by each filter is progressively shifted with the changing angle of the filter relative to the light path. The filter wheel configuration includes opaque vanes extending from the ends of the filters to periodically interrupt the passage of light to the specimen. Photocells are positioned to sense the level of light reflected from the specimen. The output of the photocells is sampled at pedetermined times relative to the rotation of the filter wheel to yield values indicative of reflected intensity at certain wavelengths. Using these values, an electronic circuit in one embodiment calculates three optical density difference values corresponding to moisture, protein and oil content of the specimen. The difference values are automatically inserted in three linear equations which are solved to obtain readings representing the true percentages of oil, water and protein contained in the specimen. Each time a new specimen is loaded for testing, the instrument is automatically calibrated against a standard sample, preferably Teflon (Trademark). The output of the photocells is amplified in a special circuit which subtracts the level of dark period current from the output when the photocells are illuminated.
Abstract:
A Raman spectroscopy system and method for determining a zero-calibration level. A gas sample chamber is located within a resonant cavity. A light source is located to cause light to be incident on the gas sample, the light resonates in the resonant cavity. Typically, the light source and resonator cavity in conjunction form a laser source which propagates coherent, monochromatic laser light energy through the gas sample. This causes Raman scattering from the gases constituent in the gas sample. The amount of Raman scattered light is measured at detectors along with light due to dark noise inherent in the detectors and glow from the laser source, i.e., light at wavelengths other than the laser light wavelength produced by the laser source. The resonator cavity is obstructed, via a ball inserted into the path of the laser beam for example, to prevent resonance. If the light source and resonant cavity in conjunction form a laser, prevention of resonance causes cessation of lasing. Thus, substantially no Raman scattering occurs and detectors measure light due primarily to background noise. The origin of the measurement scale for the detectors may then be set at the measured intensity, i.e., a zero-calibration level for the detectors may be determined.