Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase, and use thereof
    4.
    发明授权
    Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase, and use thereof 有权
    人参皂甙菌KCTC22231T衍生人参皂甙苷,其使用

    公开(公告)号:US08877463B2

    公开(公告)日:2014-11-04

    申请号:US13497042

    申请日:2010-09-20

    摘要: The present invention relates to Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase and use thereof. The polypeptide has an activity of converting PPD (protopanaxadiol)-type saponins into in vivo absorbable and highly active deglycosylated saponins, by selective hydrolysis of a bond at a particular position of ginsenoside. The present invention also relates an amino acid sequence constituting the polypeptide, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector. The invention further provides a method for preparing Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase by culturing the transformant, a method for converting PPD (protopanaxadiol)-type major ginsenoside into a rare ginsenoside that is absorbable in vivo using the protein, and a composition for converting PPD-type saponins into in vivo absorbable saponins, having the protein as an active ingredient.

    摘要翻译: 本发明涉及人参皂苷KCTC22231T衍生的人参皂苷糖苷酶及其用途。 通过选择性水解人参皂苷的特定位置的键,该多肽具有将PPD(原人参二醇)型皂苷转化成体内可吸收和高活性的去糖基化皂苷的活性。 本发明还涉及构成多肽的氨基酸序列,编码该蛋白质的核酸序列,包含该核酸序列的重组载体和用载体转化的转化体。 本发明还提供了一种通过培养转化体制备人参皂甙菌素KCTC22231T衍生的人参皂苷糖苷酶的方法,用于将PPD(原人参二醇)型主要人参皂甙转化为使用该蛋白质在体内可吸收的稀有人参皂甙的方法和用于转化的组合物 PPD型皂苷进入体内可吸收的皂苷,其具有蛋白质作为活性成分。

    Rhodanobacter Ginsenosidimutans KCTC22231T-Derived Ginsenoside Glycosidase, and Use Thereof
    6.
    发明申请
    Rhodanobacter Ginsenosidimutans KCTC22231T-Derived Ginsenoside Glycosidase, and Use Thereof 有权
    人参皂甙元KCTC22231T衍生的人参皂苷糖苷酶Rhodanobacter

    公开(公告)号:US20120190063A1

    公开(公告)日:2012-07-26

    申请号:US13497042

    申请日:2010-09-20

    摘要: The present invention relates to Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase and use thereof, and this protein has an activity of converting PPD (protopanaxadiol)-type saponins into in vivo absorbable, highly active deglycosylated saponins by selective hydrolysis of a bond at a particular position of ginsenoside. More particularly, the present invention relates to an amino acid sequence constituting the protein, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, a transformant transformed with the vector, a method for preparing Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase by culturing the transformant, a method for converting PPD (protopanaxadiol)-type major ginsenoside into a rare ginsenoside that is absorbable in vivo using the protein, and a composition for converting PPD-type saponins into in vivo absorbable saponins, comprising the protein as an active ingredient.

    摘要翻译: 本发明涉及人参皂苷KCTC22231T衍生的人参皂苷糖苷酶及其用途,该蛋白质具有通过选择性水解特定位置的键而将PPD(原人参二醇)型皂甙转化为体内可吸收,高活性的脱糖基化皂苷的活性 的人参皂苷。 更具体地说,本发明涉及构成蛋白质的氨基酸序列,编码该蛋白质的核酸序列,包含该核酸序列的重组载体,用该载体转化的转化体,一种制备人参皂甙的人参皂苷KCTC22231T的人参皂甙 通过培养转化体的糖苷酶,将PPD(原人参二醇)型主要人参皂甙转化成能够使用蛋白质在体内可吸收的稀有人参皂苷的方法,以及将PPD型皂甙转化为体内可吸收皂苷的组合物,其含有蛋白质 活性成分。

    Method of producing a plaque dispersing enzyme
    8.
    发明授权
    Method of producing a plaque dispersing enzyme 失效
    生产斑块分散酶的方法

    公开(公告)号:US4328313A

    公开(公告)日:1982-05-04

    申请号:US232595

    申请日:1981-02-09

    IPC分类号: A61K38/00 C12N9/24

    摘要: A method of producing .alpha.-1, 3-glucanase by introducing a bacterial culture such as Pseudomonas sp. isolate NRRL B-12324 into an aqueous medium containing a "limit glucan" substrate which is greater than 90 percent .alpha.-1, 3-glycosidically linked, then allowing growth to take place to accumulate .alpha.-1, 3-glucanase, and then recovering the enzyme for use as an oral therapeutic agent.

    摘要翻译: 通过引入细菌培养物如假单胞菌属(Pseudomonas sp。)来产生α-1,3-葡聚糖酶的方法。 将NRRL B-12324分离成含有“极性葡聚糖”底物的水性培养基,其大于90%的α-1,3-糖苷连接,然后允许生长以积累α-1,3-葡聚糖酶,然后回收 用作口服治疗剂的酶。