公开(公告)号：US20120264167A1

公开(公告)日：2012-10-18

申请号：US13453893

申请日：2012-04-23

申请人： Dong Shan AN ,  Song Gun KIM ,  Sung Taik LEE ,  Wan Taek IM ,  Hyung Gwan LEE ,  Sun Chang KIM

发明人： Dong Shan AN ,  Song Gun KIM ,  Sung Taik LEE ,  Wan Taek IM ,  Hyung Gwan LEE ,  Sun Chang KIM

IPC分类号： C12N9/24 ,  C12N15/70 ,  C12P33/20 ,  C12N1/21

CPC分类号： C12P33/00 ,  C12N9/2445 ,  C12Y302/01021

摘要： The present invention relates to a novel ginsenoside glycosidase protein derived from the genus Terrabacter, the protein having an activity of converting protopanaxadiol (PPD)-type saponins into highly active substances, which can be absorbed inside the body, by selective hydrolysis of a particular bond of ginsenoside. More specifically, the present invention relates to an amino acid sequence of the protein, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector, and a method for producing ginsenoside glycosidase derived from the genus Terrabacter by culturing the transformant, a method for converting PPD-type major saponins into the minor saponin forms using the protein, and a composition for converting PPD-type saponins into soluble saponins, comprising the protein as an active component.

摘要翻译： 本发明涉及一种衍生自Terrabacter属的新型人参皂苷糖苷酶蛋白质，该蛋白质具有将原人参二醇（PPD）型皂甙转化为高活性物质的活性，其可通过选择性水解特定的键 的人参皂苷。 更具体地说，本发明涉及蛋白质的氨基酸序列，编码该蛋白质的核酸序列，包含该核酸序列的重组载体和用该载体转化的转化体，以及一种用于生产人参皂苷糖苷酶的方法，其衍生自 通过培养转化体的Terrabacter属，使用该蛋白质将PPD型主皂苷转化成次皂苷形式的方法，以及将PPD型皂苷转化为可溶性皂苷的组合物，其包含该蛋白质作为活性成分。

公开(公告)号：US08735129B2

公开(公告)日：2014-05-27

申请号：US13453893

申请日：2012-04-23

申请人： Dong Shan An ,  Song Gun Kim ,  Sung Taik Lee ,  Wan Taek Im ,  Hyung Gwan Lee ,  Sun Chang Kim

发明人： Dong Shan An ,  Song Gun Kim ,  Sung Taik Lee ,  Wan Taek Im ,  Hyung Gwan Lee ,  Sun Chang Kim

IPC分类号： C12N9/24 ,  C12P33/20 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12P33/00 ,  C12N9/2445 ,  C12Y302/01021

摘要： The present invention relates to a novel ginsenoside glycosidase protein derived from the genus Terrabacter, the protein having an activity of converting protopanaxadiol (PPD)-type saponins into highly active substances, which can be absorbed inside the body, by selective hydrolysis of a particular bond of ginsenoside. More specifically, the present invention relates to an amino acid sequence of the protein, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector, and a method for producing ginsenoside glycosidase derived from the genus Terrabacter by culturing the transformant, a method for converting PPD-type major saponins into the minor saponin forms using the protein, and a composition for converting PPD-type saponins into soluble saponins, comprising the protein as an active component.

摘要翻译： 本发明涉及一种衍生自Terrabacter属的新型人参皂苷糖苷酶蛋白质，该蛋白质具有将原人参二醇（PPD）型皂甙转化为高活性物质的活性，其可通过选择性水解特定的键 的人参皂苷。 更具体地说，本发明涉及蛋白质的氨基酸序列，编码该蛋白质的核酸序列，包含该核酸序列的重组载体和用该载体转化的转化体，以及一种用于生产人参皂苷糖苷酶的方法，其衍生自 通过培养转化体的Terrabacter属，使用该蛋白质将PPD型主皂苷转化成次皂苷形式的方法，以及将PPD型皂苷转化为可溶性皂苷的组合物，其包含该蛋白质作为活性成分。

公开(公告)号：US20120190063A1

公开(公告)日：2012-07-26

申请号：US13497042

申请日：2010-09-20

申请人： Dong-Shan An ,  Song-Gun Kim ,  Sung-Taik Lee ,  Wan-Taek Im ,  Chang-Hao Cui

发明人： Dong-Shan An ,  Song-Gun Kim ,  Sung-Taik Lee ,  Wan-Taek Im ,  Chang-Hao Cui

IPC分类号： C12P33/20 ,  C12N9/24 ,  C12N1/21 ,  C12N15/56 ,  C12N15/63

CPC分类号： C12P19/56 ,  A61K31/704 ,  C12N9/2402 ,  C12N9/2445 ,  C12Y302/01021 ,  C12Y302/01071 ,  C12Y302/01075 ,  C12Y302/01088

摘要： The present invention relates to Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase and use thereof, and this protein has an activity of converting PPD (protopanaxadiol)-type saponins into in vivo absorbable, highly active deglycosylated saponins by selective hydrolysis of a bond at a particular position of ginsenoside. More particularly, the present invention relates to an amino acid sequence constituting the protein, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, a transformant transformed with the vector, a method for preparing Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase by culturing the transformant, a method for converting PPD (protopanaxadiol)-type major ginsenoside into a rare ginsenoside that is absorbable in vivo using the protein, and a composition for converting PPD-type saponins into in vivo absorbable saponins, comprising the protein as an active ingredient.

摘要翻译： 本发明涉及人参皂苷KCTC22231T衍生的人参皂苷糖苷酶及其用途，该蛋白质具有通过选择性水解特定位置的键而将PPD（原人参二醇）型皂甙转化为体内可吸收，高活性的脱糖基化皂苷的活性 的人参皂苷。 更具体地说，本发明涉及构成蛋白质的氨基酸序列，编码该蛋白质的核酸序列，包含该核酸序列的重组载体，用该载体转化的转化体，一种制备人参皂甙的人参皂苷KCTC22231T的人参皂甙 通过培养转化体的糖苷酶，将PPD（原人参二醇）型主要人参皂甙转化成能够使用蛋白质在体内可吸收的稀有人参皂苷的方法，以及将PPD型皂甙转化为体内可吸收皂苷的组合物，其含有蛋白质 活性成分。

公开(公告)号：US08877463B2

公开(公告)日：2014-11-04

申请号：US13497042

申请日：2010-09-20

申请人： Dong-Shan An ,  Song-Gun Kim ,  Sung-Taik Lee ,  Wan-Taek Im ,  Chang-Hao Cui

发明人： Dong-Shan An ,  Song-Gun Kim ,  Sung-Taik Lee ,  Wan-Taek Im ,  Chang-Hao Cui

IPC分类号： C12P19/56 ,  C12N9/24 ,  C12N1/21 ,  C12N15/56 ,  C12N15/63 ,  C12N15/70 ,  C12N9/42 ,  A61K31/704

CPC分类号： C12P19/56 ,  A61K31/704 ,  C12N9/2402 ,  C12N9/2445 ,  C12Y302/01021 ,  C12Y302/01071 ,  C12Y302/01075 ,  C12Y302/01088

摘要： The present invention relates to Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase and use thereof. The polypeptide has an activity of converting PPD (protopanaxadiol)-type saponins into in vivo absorbable and highly active deglycosylated saponins, by selective hydrolysis of a bond at a particular position of ginsenoside. The present invention also relates an amino acid sequence constituting the polypeptide, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector. The invention further provides a method for preparing Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase by culturing the transformant, a method for converting PPD (protopanaxadiol)-type major ginsenoside into a rare ginsenoside that is absorbable in vivo using the protein, and a composition for converting PPD-type saponins into in vivo absorbable saponins, having the protein as an active ingredient.

摘要翻译： 本发明涉及人参皂苷KCTC22231T衍生的人参皂苷糖苷酶及其用途。 通过选择性水解人参皂苷的特定位置的键，该多肽具有将PPD（原人参二醇）型皂苷转化成体内可吸收和高活性的去糖基化皂苷的活性。 本发明还涉及构成多肽的氨基酸序列，编码该蛋白质的核酸序列，包含该核酸序列的重组载体和用载体转化的转化体。 本发明还提供了一种通过培养转化体制备人参皂甙菌素KCTC22231T衍生的人参皂苷糖苷酶的方法，用于将PPD（原人参二醇）型主要人参皂甙转化为使用该蛋白质在体内可吸收的稀有人参皂甙的方法和用于转化的组合物 PPD型皂苷进入体内可吸收的皂苷，其具有蛋白质作为活性成分。