知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

25 条记录 (耗时 0.018 秒)

    Fermentative Production of Oligosaccharides
    4.
    发明申请
    Fermentative Production of Oligosaccharides 审中-公开
    低聚糖的发酵生产

    公开(公告)号：US20160215315A1

    公开(公告)日：2016-07-28

    申请号：US14914520

    申请日：2014-09-05

    申请人： Glycom A/S

    发明人： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

    IPC分类号： C12P19/18 ,  C12P19/26

    CPC分类号： C12P19/18 ,  C07H3/06 ,  C08B37/006 ,  C12P19/00 ,  C12P19/26 ,  C12Y204/01065 ,  C12Y204/01087 ,  C12Y204/01099

    摘要： The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.

    摘要翻译： 本申请公开了一种生产至少四个单糖单元的寡糖的方法，有利地是HMO，特别是仅四个单糖单元的HMO，所述方法包括以下步骤：在含有岩藻糖基化的唾液酸化或N- 乙酰基 - 葡糖胺化的乳糖三糖作为受体，一种遗传修饰的细胞，其具有编码能够修饰至少四个单糖单元的寡糖的生物合成途径中的所述受体或必需中间体的酶的重组基因，有利地是HMO，特别是 来自所述受体的仅四种单糖单元的HMO。

    Preparation and uses of gene sequences encoding chimerical glycosyltransferases with optimized glycosylation activity
    5.
    发明授权
    Preparation and uses of gene sequences encoding chimerical glycosyltransferases with optimized glycosylation activity 有权
    编码和使用编码具有优化糖基化活性的嵌合糖基转移酶的基因序列

    公开(公告)号：US08993297B2

    公开(公告)日：2015-03-31

    申请号：US12301914

    申请日：2007-05-24

    申请人： Catherine Ronin ,  Gaelle Guiraudie-Capraz

    发明人： Catherine Ronin ,  Gaelle Guiraudie-Capraz

    IPC分类号： C12N9/10 ,  C12P21/06 ,  C12P21/04 ,  C12N9/24 ,  C12N5/00 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N9/1048 ,  C07K2319/05 ,  C12N9/1051 ,  C12N9/1081 ,  C12P21/005 ,  C12Y204/01087 ,  C12Y204/01133 ,  C12Y204/99001

    摘要： The present invention relates to the production of gene sequences encoding chimerical membrane glycosyltransferases presenting an optimized glycosylation activity in cells transformed with the sequences, the gene sequences corresponding to the fusion: of a first nucleic acid coding for a C-terminal minimal fragment of the catalytic domain (CD) of the native full length glycosyltransferase, to a second nucleic acid coding for a transmembrane peptide comprising in its N-terminal region a cytoplasmic tail (CT) region located upstream from a transmembrane domain (TMD), itself located upstream of a stem region (SR), provided that at least one of these CT, TMD, SR peptides being different from the primary structure of the naturally occurring peptide counterparts present in the native glycosyltransferase from which is derived the CD fragment with optimal glycosyltransferase activity as defined above.

    摘要翻译： 本发明涉及编码嵌合膜糖基转移酶的基因序列的生产，其在用序列转化的细胞中呈现优化的糖基化活性，对应于融合的基因序列：编码催化的C末端最小片段的第一核酸 将天然全长糖基转移酶的结构域（CD）转移到编码跨膜肽的第二核酸，其在其N末端区域中包含位于跨膜结构域（TMD）上游的胞质尾（CT）区域，其本身位于 茎区域（SR），条件是这些CT，TMD，SR肽中的至少一种不同于存在于天然糖基转移酶中的天然存在的肽对应物的一级结构，其中衍生自具有如上定义的最佳糖基转移酶活性的CD片段 。

    PREPARATION AND USES OF GENE SEQUENCES ENCODING CHIMERICAL GLYCOSYLTRANSFERASES WITH OPTIMIZED GLYCOSYLATION ACTIVITY
    6.
    发明申请
    PREPARATION AND USES OF GENE SEQUENCES ENCODING CHIMERICAL GLYCOSYLTRANSFERASES WITH OPTIMIZED GLYCOSYLATION ACTIVITY 有权
    用优化的糖苷酶活性编码糖原水解酶的基因序列的制备和用途

    公开(公告)号：US20100105106A1

    公开(公告)日：2010-04-29

    申请号：US12301914

    申请日：2007-05-24

    申请人： Catherine Ronin ,  Gaelle Guiraudie-Capraz

    发明人： Catherine Ronin ,  Gaelle Guiraudie-Capraz

    IPC分类号： C12P21/06 ,  C12P19/34 ,  C12N15/54 ,  C12N15/63 ,  C12N5/10 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19

    CPC分类号： C12N9/1048 ,  C07K2319/05 ,  C12N9/1051 ,  C12N9/1081 ,  C12P21/005 ,  C12Y204/01087 ,  C12Y204/01133 ,  C12Y204/99001

    摘要： The present invention relates to the production of gene sequences encoding chimerical membrane glycosyltransferases presenting an optimized glycosylation activity in cells transformed with said sequences, said gene sequences corresponding to the fusion: of a first nucleic acid coding for a C-terminal minimal fragment of the catalytic domain (CD) of the native full length glycosyltransferase, to a second nucleic acid coding for a transmembrane peptide comprising in its N-terminal region a cytoplasmic tail (CT) region located upstream from a transmembrane domain (TMD), itself located upstream of a stem region (SR), provided that at least one of these CT, TMD, SR peptides being different from the primary structure of the naturally occurring peptide counterparts present in the native glycosyltransferase from which is derived the CD fragment with optimal glycosyltransferase activity as defined above. The invention also relates to the use of said gene sequences in the frame of the preparation of recombinant proteins of interest by cells transformed with said sequences and sequences encoding said recombinant proteins.

    摘要翻译： 本发明涉及编码嵌合膜糖基转移酶的基因序列的产生，其在用所述序列转化的细胞中呈现优化的糖基化活性，所述基因序列对应于编码催化的C末端最小片段的第一个核酸 将天然全长糖基转移酶的结构域（CD）转移到编码跨膜肽的第二核酸，其在其N末端区域中包含位于跨膜结构域（TMD）上游的胞质尾（CT）区域，其本身位于 茎区域（SR），条件是这些CT，TMD，SR肽中的至少一种不同于存在于天然糖基转移酶中的天然存在的肽对应物的一级结构，其中衍生自具有如上定义的最佳糖基转移酶活性的CD片段 。 本发明还涉及在用所述序列转化的细胞和编码所述重组蛋白的序列的制备重组蛋白质的框架中使用所述基因序列。

    Selective antibody targeting of undifferentiated stem cells
    10.
    发明授权
    Selective antibody targeting of undifferentiated stem cells 失效
    未分化干细胞的选择性抗体靶向

    公开(公告)号：US06921665B2

    公开(公告)日：2005-07-26

    申请号：US09995419

    申请日：2001-11-26

    申请人： Jim McWhir ,  Joseph D. Gold ,  J. Michael Schiff

    发明人： Jim McWhir ,  Joseph D. Gold ,  J. Michael Schiff

    IPC分类号： A61K35/12 ,  A61K48/00 ,  C12N5/0735 ,  C12N9/10 ,  C12N15/54 ,  A01N1/00 ,  A01N1/02 ,  G01N33/53 ,  C12P1/00 ,  C12N15/63

    CPC分类号： C12N9/1276 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0606 ,  C12N9/1048 ,  C12N2502/13 ,  C12N2510/00 ,  C12N2799/022 ,  C12Y204/01087

    摘要： This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in relatively undifferentiated cells in the population. Expression of the effector gene results in expression of a cell-surface antigen that can be used to deplete the undifferentiated cells. Model effector sequences encode glycosyl transferases that synthesize carbohydrate xenoantigen or alloantigen, which can be used for immunoseparation or as a target for complement-mediated lysis. The differentiated cell populations produced are suitable for use in tissue regeneration and non-therapeutic applications such as drug screening.

    摘要翻译： 本发明提供了一种用于从干细胞群体产生分化细胞的系统，用于需要相对均匀细胞群体的地方。 细胞含有在转录控制元件（例如TERT启动子）的控制下的效应基因，其导致基因在群体中相对未分化的细胞中表达。 效应基因的表达导致可用于消耗未分化细胞的细胞表面抗原的表达。 模型效应序列编码糖基转移酶，其合成碳水化合物异种抗原或同种异体抗原，其可用于免疫分离或作为补体介导的裂解的靶标。 产生的分化细胞群体适用于组织再生和非治疗应用，如药物筛选。