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    MODIFIED AMADORIASE AND METHOD FOR PRODUCING THE SAME, AGENT FOR IMPROVING SURFACTANT RESISTANCE OF AMADORIASE AND COMPOSITION FOR MEASURING HbA1c USING THE SAME
    2.
    发明申请
    MODIFIED AMADORIASE AND METHOD FOR PRODUCING THE SAME, AGENT FOR IMPROVING SURFACTANT RESISTANCE OF AMADORIASE AND COMPOSITION FOR MEASURING HbA1c USING THE SAME 审中-公开
    改性助剂及其制备方法，用于改善丙烯酸酯的耐表面活性剂的组合物和用于测量HbA1c的组合物

    公开(公告)号：US20160186232A1

    公开(公告)日：2016-06-30

    申请号：US14910789

    申请日：2014-08-08

    申请人： Kikkoman Corporation

    发明人： Yosuke MASAKARI ,  Atsushi ICHIYANAGI

    IPC分类号： C12Q1/26 ,  C12N9/06

    CPC分类号： C12Q1/26 ,  C12N9/0032 ,  C12Y105/03 ,  G01N33/723 ,  G01N2333/805 ,  G01N2333/90672

    摘要： Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.

    摘要翻译： 提供了即使在比常规情况更强的表面活性剂存在下也可以测量糖化血红蛋白的组合物。 还提供了保持阿马道里酶的残留活性或降低残留活性降低的缓冲剂和/或稳定剂。 本发明提供一种用于测量糖化血红蛋白的组合物，该组合物含有阿马道里酶，该阿马双利酵母在与选自位置262，位置257，氨基酸和氨基酸的氨基酸相对应的位置具有一个或多个氨基酸残基的取代， 来源于Coniochaeta的阿马多利亚酶的位置249，位置253，位置337，位置340，位置232，位置129，位置132，位置133，位置44，位置256，位置231和位置81，由SEQ ID NO： 1或3，即使在表面活性剂存在下也具有残留活性。 本发明还提供了用于测量糖化血红蛋白的组合物和试剂盒，其包含特异性稳定剂和/或缓冲液。 本发明可以提供用于测量糖化血红蛋白的酶和组合物，即使它们暴露于表面活性剂也具有优异的储存稳定性。

    ENZYMATIC DETERMINATION OF HBA1C
    3.
    发明申请
    ENZYMATIC DETERMINATION OF HBA1C 审中-公开
    HBA1C的酶学测定

    公开(公告)号：US20170058318A1

    公开(公告)日：2017-03-02

    申请号：US15308531

    申请日：2015-05-06

    申请人： DiaSys Diagnostic Systems GmbH

    发明人： Günther GORKA ,  Yoshifumi WATAZU ,  Erwin METZMANN ,  Alexandra LEIN ,  Holger MÜLLER ,  Matthias GRIMMLER

    IPC分类号： C12Q1/37 ,  C12Q1/26

    CPC分类号： C12Q1/28 ,  C12N9/6491 ,  C12Q1/26 ,  C12Q1/37 ,  C12Q2326/00 ,  C12Y105/03 ,  C12Y304/24027 ,  G01N33/723 ,  G01N33/725 ,  G01N2333/805 ,  G01N2333/90672 ,  G01N2333/954

    摘要： A method for determining the amount of glycated haemoglobin (HbA1c), in which—if required—the erythrocytes in a sample are haemolysed, the haemoglobin that is then released—if required—is contacted with a proteolytic agent and the glycated haemoglobin degradation products obtained in this way or otherwise are quantified is disclosed. In order to provide such a process and reagents employable therein that has/have the property of sufficient stability of the chemical compounds that are essential to the reaction, for the stabilization of the haemoglobin which is unfolded at a very low pH in the range from 1 to 3, at least one suitable stabilizer is present in the haemolysis solution. Where a leuco dye is used in connection with the determination of the amount of HbA1c, it is proposed that the latter be stabilized with particular phosphine compounds and/or thio compounds, and, in particular embodiments, the requisite proteolytic agent is to be provided in the form of an inactivated protease which is then only reactivated in situ.

    摘要翻译： 用于测定糖化血红蛋白（HbA1c）量的方法，其中 - 如果需要的话，样品中的红细胞被溶血，然后释放的血红蛋白（如果需要的话）与蛋白水解剂接触，并获得糖化血红蛋白降解产物 以此方式或以其他方式量化。 为了提供其中可使用的具有/具有对反应必需的化学化合物具有足够稳定性的性质的方法和试剂，为了稳定在非常低的pH下展开的血红蛋白的范围为1 至3，溶血溶液中存在至少一种合适的稳定剂。 当与测定HbA1c的量相关联使用无色染料时，建议后者用特定的膦化合物和/或硫代化合物稳定，并且在具体实施方案中，所需的蛋白水解剂将被提供在 灭活的蛋白酶的形式仅在原位再活化。

    AMADORIASE WITH IMPROVED THERMOSTABILITY, GENE AND RECOMBINANT DNA FOR THE AMADORIASE, AND METHOD FOR PRODUCTION OF AMADORIASE WITH IMPROVED THERMOSTABILITY
    4.
    发明申请
    AMADORIASE WITH IMPROVED THERMOSTABILITY, GENE AND RECOMBINANT DNA FOR THE AMADORIASE, AND METHOD FOR PRODUCTION OF AMADORIASE WITH IMPROVED THERMOSTABILITY 有权
    具有改进的热稳定性的改良剂，用于赋形剂的基因和重组DNA，以及具有改善的热稳定性的用于生产甲醛的方法

    公开(公告)号：US20140356928A1

    公开(公告)日：2014-12-04

    申请号：US14369192

    申请日：2012-12-27

    申请人： KIKKOMAN CORPORATION

    发明人： Yosuke Masakari ,  Atsushi Ichiyanagi

    IPC分类号： C12N9/06

    CPC分类号： C12N9/0032 ,  C12Y105/03

    摘要： An amadoriase having a substitution or a deletion of one or more amino acid residues at positions corresponding to amino acids selected from the group consisting of three amino acid residues from the carboxyl terminal and amino acids at positions 151, 43, 53, 267, 350, 185, 196, 299 and 323 in the amino acid sequence of amadoriase derived from the Coniochaeta species indicated in SEQ ID NO: 1. The amadoriase having a heat resistance which is superior to that of a conventional amadoriase.

    摘要翻译： 在对应于选自羧基末端的三个氨基酸残基的氨基酸的位置和位置151,43,53,267,350的氨基酸处具有取代或缺失一个或多个氨基酸残基的阿马道里酶， 185,296,29和323在源自SEQ ID NO：1所示的Coniochaeta物种的阿马道里酶的氨基酸序列中。具有优于常规阿马道里酶的耐热性的阿马道里酶。

    FRUCTOSE AMINO ACID OXIDASE, PREPARATION METHOD AND ENZYME-CONTAINING KIT FOR DETECTING GLYCATED ALBUMIN
    7.
    发明申请
    FRUCTOSE AMINO ACID OXIDASE, PREPARATION METHOD AND ENZYME-CONTAINING KIT FOR DETECTING GLYCATED ALBUMIN 有权
    纤维素氨基酸氧化酶，用于检测糖化铝的制备方法和含酶包装试剂盒

    公开(公告)号：US20160319248A1

    公开(公告)日：2016-11-03

    申请号：US15108320

    申请日：2014-12-11

    申请人： NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO., LTD.

    发明人： Bingde ZOU ,  Jihua ZOU ,  Yi WANG ,  Jianghua JIA

    IPC分类号： C12N9/06 ,  G01N33/68

    CPC分类号： C12N9/0032 ,  C12Y105/03 ,  G01N33/6842 ,  G01N2333/765 ,  G01N2333/90638 ,  G01N2333/90672

    摘要： Fructosyl amino acid oxidase is provided, which has an amino acid sequence as shown in SEQID. No. 1 or fructosyl amino acid oxidase having a homology of more than 80% with this amino acid sequence, on a corresponding site of an amino acid selected from following (a) to (f), having one or more amino acid residues conducting a substitution, obtained fructosyl amino acid oxidase having a higher thermostability: (a) 59-site glutamic acid, (b) 98-site glutamic acid, (c) 225-site glycine, (d) 277-site lysine, (e) 285-site glutamic acid, and (f) 355-site aspartic acid. The method for preparing the above oxidase and the test kit containing the enzyme for determining glycated albumin are also provided.

    摘要翻译： 提供了果糖基氨基酸氧化酶，其具有如SEQID所示的氨基酸序列。 1或与该氨基酸序列具有大于80％同源性的果糖基氨基酸氧化酶，在选自以下（a）至（f）的氨基酸的相应位点上具有一个或多个氨基酸残基进行 取代，得到具有较高热稳定性的果糖基氨基酸氧化酶：（a）59位谷氨酸，（b）98位谷氨酸，（c）225位点甘氨酸，（d）277位点赖氨酸，（e）285 谷氨酸，（f）355位天冬氨酸。 还提供了制备上述氧化酶的方法和含有用于测定糖化白蛋白的酶的测试试剂盒。

    HEMOGLOBIN A1c MEASUREMENT METHOD AND MEASUREMENT KIT
    8.
    发明申请
    HEMOGLOBIN A1c MEASUREMENT METHOD AND MEASUREMENT KIT 审中-公开
    HEMOGLOBIN A1c测量方法和测量套件

    公开(公告)号：US20160251695A1

    公开(公告)日：2016-09-01

    申请号：US15031037

    申请日：2014-10-24

    申请人： Kikkoman Corporation

    发明人： Yosuke MASAKARI ,  Atsushi ICHIYANAGI

    IPC分类号： C12Q1/26 ,  C12Q1/28 ,  G01N21/78 ,  C12N9/06

    CPC分类号： C12Q1/26 ,  C12N9/0032 ,  C12Q1/28 ,  C12Y105/03 ,  C12Y111/01 ,  G01N21/78 ,  G01N33/725 ,  G01N2333/805 ,  G01N2333/90672 ,  G01N2333/908

    摘要： This invention provides an amadoriase that acts on the β-chain of hemoglobin A1c (HbA1c) and generates hydrogen peroxide, a method for measurement of HbA1c using such amadoriase, and a reagent kit for measurement of HbA1c using such amadoriase. The method for measurement of HbA1c involves the use of an amadoriase that has specific activity of 0.1 U/mg or greater to αF6P and oxidizes the HbA1c β-chain amino terminus so as to generate hydrogen peroxide, and the reagent kit for measurement of HbA1c comprises such amadoriase. The method and the kit for measurement of HbA1c enable quantification of HbA1c to be performed rapidly, simply, and accurately.

    摘要翻译： 本发明提供了作用于血红蛋白A1c（HbA1c）的β链并产生过氧化氢的阿马曲利酶，使用这种阿马道里酶测量HbA1c的方法和使用这种阿马道里酶测量HbA1c的试剂盒。 HbA1c的测定方法涉及使用比α活性为0.1U / mg以上的阿莫地利酶，并使HbA1c的β-氨基末端氧化以产生过氧化氢，而用于测定HbA1c的试剂盒包括 这样的阿马道里苷。 用于测量HbA1c的方法和试剂盒使HbA1c的定量能够快速，简单，准确地进行。