公开(公告)号：US20240102075A1

公开(公告)日：2024-03-28

申请号：US18538143

申请日：2023-12-13

申请人： KIKKOMAN CORPORATION

发明人： Kanako HAYASHI ,  Atsushi ICHIYANAGI

IPC分类号： C12Q1/26 ,  G01N27/327

CPC分类号： C12Q1/26 ,  G01N27/327

摘要： A novel method for quantifying a 25-hydroxyvitamin D concentration, a hydroxylase, a quantification composition, a quantification kit, an electrode, a sensor chip, and a sensor are provided. A method for quantifying 25-hydroxyvitamin D by adding a mediator and hydroxylase to a sample can be provided. Quantification of an oxidized mediator produced by an action of the hydroxylase, or oxygen or a reduced mediator consumed by an action of the hydroxylase may be included.

公开(公告)号：US20220308074A1

公开(公告)日：2022-09-29

申请号：US17837142

申请日：2022-06-10

申请人： KIKKOMAN CORPORATION

发明人： Kanako HAYASHI ,  Atsushi ICHIYANAGI

IPC分类号： G01N33/82 ,  G01N33/52 ,  C12N9/02

摘要： A quantitation method of vitamin D derivative is provided including adding an oxidoreductase to a sample. The quantitation method of vitamin D derivative may further include reducing a mediator by adding the oxidoreductase, and reacting the reduced mediator with a reagent to determine a concentration of the vitamin D derivative.

公开(公告)号：US20200263228A1

公开(公告)日：2020-08-20

申请号：US16832712

申请日：2020-03-27

申请人： Kikkoman Corporation

发明人： Yosuke MASAKARI ,  Atsushi ICHIYANAGI

IPC分类号： C12Q1/26 ,  G01N33/72 ,  C12N9/06

摘要： Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.

公开(公告)号：US20230213499A1

公开(公告)日：2023-07-06

申请号：US17801033

申请日：2021-02-18

申请人： KIKKOMAN CORPORATION

发明人： Yosuke MASAKARI ,  Chiaki INAMOTO ,  Miki NAKAMURA ,  Eiichiro KAN ,  Atsushi ICHIYANAGI ,  Haruka HIRAGUCHI ,  Ryota SUZUKI

IPC分类号： G01N33/50 ,  C12Q1/32

CPC分类号： G01N33/50 ,  C12Q1/32 ,  G01N2333/904 ,  C12Q2520/00 ,  C12Q2521/50

摘要： An object of the present invention is to provide a device for evaluating a state of interstitial fluid, blood, urine, tears, sweat, saliva of human and non-human organisms, foods and drinks, brewed product, etc., a system, a program, and an evaluation method using these.
The present invention is to provide a device for evaluating a state of a sample which comprises an action part for allowing lactate dehydrogenase to act on a sample, and a sensor for sensing the state of the sample on which lactate dehydrogenase is allowed to act, a system, a program, a method for evaluating the state of the sample using these, and an enzyme to be used therefor.

公开(公告)号：US20230203559A1

公开(公告)日：2023-06-29

申请号：US18067158

申请日：2022-12-16

申请人： KIKKOMAN CORPORATION

发明人： Haruka HIRAGUCHI ,  Atsushi ICHIYANAGI

IPC分类号： C12Q1/26 ,  C12Q1/42 ,  C12Q1/00

CPC分类号： C12Q1/26 ,  C12Q1/42 ,  C12Q1/005

摘要： A quantitation method of ethanolamine phosphate capable of measuring trace amounts of ethanolamine phosphate, or oxidoreductase used for the quantitation method thereof, a quantitation composition, a quantitation kit, a sensor chip, or a sensor is provided. According to an embodiment of the present invention, a quantitation method of ethanolamine phosphate including allowing phosphatase to act on a sample to convert ethanolamine phosphate contained in the sample into ethanolamine, and allowing oxidoreductase to act on the ethanolamine is provided.

公开(公告)号：US20210171919A1

公开(公告)日：2021-06-10

申请号：US17257745

申请日：2019-07-05

申请人： Kikkoman Corporation

发明人： Kanako HAYASHI ,  Atsushi ICHIYANAGI

IPC分类号： C12N9/02 ,  C12Q1/66

摘要： In one embodiment, an object of the present invention is to provide a firefly luciferase having improved thermostability. In one embodiment, the present invention relates to a luciferase mutant having improved thermostability that is a mutant of firefly luciferase comprising an amino acid sequence in which the amino acid residue at the position corresponding to position 393 of SEQ ID NO 1 is substituted, a polynucleotide encoding the luciferase mutant, and a production method of the luciferase mutant.

公开(公告)号：US20160186232A1

公开(公告)日：2016-06-30

申请号：US14910789

申请日：2014-08-08

申请人： Kikkoman Corporation

发明人： Yosuke MASAKARI ,  Atsushi ICHIYANAGI

IPC分类号： C12Q1/26 ,  C12N9/06

CPC分类号： C12Q1/26 ,  C12N9/0032 ,  C12Y105/03 ,  G01N33/723 ,  G01N2333/805 ,  G01N2333/90672

摘要： Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.

摘要翻译： 提供了即使在比常规情况更强的表面活性剂存在下也可以测量糖化血红蛋白的组合物。 还提供了保持阿马道里酶的残留活性或降低残留活性降低的缓冲剂和/或稳定剂。 本发明提供一种用于测量糖化血红蛋白的组合物，该组合物含有阿马道里酶，该阿马双利酵母在与选自位置262，位置257，氨基酸和氨基酸的氨基酸相对应的位置具有一个或多个氨基酸残基的取代， 来源于Coniochaeta的阿马多利亚酶的位置249，位置253，位置337，位置340，位置232，位置129，位置132，位置133，位置44，位置256，位置231和位置81，由SEQ ID NO： 1或3，即使在表面活性剂存在下也具有残留活性。 本发明还提供了用于测量糖化血红蛋白的组合物和试剂盒，其包含特异性稳定剂和/或缓冲液。 本发明可以提供用于测量糖化血红蛋白的酶和组合物，即使它们暴露于表面活性剂也具有优异的储存稳定性。

公开(公告)号：US20210310043A1

公开(公告)日：2021-10-07

申请号：US17345187

申请日：2021-06-11

申请人： KIKKOMAN CORPORATION

发明人： Atsushi ICHIYANAGI ,  Yosuke MASAKARI ,  Haruka HIRAGUCHI

IPC分类号： C12Q1/52 ,  C12Q1/26 ,  C12N9/06

摘要： There is provided a novel quantification method for quantifying a concentration of EAP, which is a biomarker of depression, an enzyme for quantitation, a composition for quantitation, a kit for quantitation or a sensor for quantitation. There is provided a quantification method of ethanolamine phosphate by adding oxidoreductase to a sample containing ethanolamine phosphate. A mediator may be reduced by adding the oxidoreductase, and the reduced mediator may be reacted with a reagent to determine a concentration of ethanolamine phosphate. In addition, hydrogen peroxide produced by adding the oxidase as the oxidoreductase may be reacted with a reagent to determine a concentration of the ethanolamine phosphate.

公开(公告)号：US20180371429A1

公开(公告)日：2018-12-27

申请号：US15563054

申请日：2016-04-04

申请人： Kikkoman Corporation

发明人： Atsushi ICHIYANAGI ,  Airi KOMATSUZAKI

IPC分类号： C12N9/06 ,  G01N33/72

摘要： This invention provides an amadoriase having improved specific activity on a glycated substrate, compared with conventional amadoriase. Provided is an amadoriase comprising a substitution of the amino acid at the position corresponding to position 64 of the amino acid sequence as shown in SEQ ID NO: 1 with an amino acid selected from the group consisting of glycine, serine, methionine, leucine, threonine, valine, and isoleucine, a method for measurement of HbA1c, and a reagent kit for measurement of HbA1c using such amadoriase. Such method and kit for measurement enable rapid, simple, and accurate quantification of HbA1c.

公开(公告)号：US20170355967A1

公开(公告)日：2017-12-14

申请号：US15521104

申请日：2015-10-23

申请人： Kikkoman Corporation

发明人： Yosuke Masakari ,  Airi KOMATSUZAKI ,  Atsushi ICHIYANAGI

IPC分类号： C12N9/06 ,  G01N27/327 ,  C12Q1/26 ,  C12Q1/00

CPC分类号： C12N9/0032 ,  C12M1/40 ,  C12Q1/004 ,  C12Q1/005 ,  C12Q1/26 ,  C12Y105/03 ,  G01N27/327 ,  G01N27/3272 ,  G01N27/3275 ,  G01N27/416 ,  G01N2333/805 ,  G01N2333/90672

摘要： Provided are an amadoriase that is less likely to be influenced by oxygen concentration and a method and a reagent kit for measurement of HbA1c using such amadoriase. Provided are an amadoriase that is obtained via substitution of one or more amino acid residues at a position or positions corresponding to the position(s) selected from the group consisting of positions 280, 267, 269, 54, and 241 of the amadoriase derived from the genus Coniochaeta, a method for measurement of HbA1c, a reagent kit for measurement, and a sensor using such amadoriase. The modified amadoriase according to the invention has a lowered oxidase activity and an enhanced dehydrogenase activity, and this enables the use of an electron mediator, and this reduces the influence of oxygen concentration. Thus, HbA1c can be measured with high sensitivity.