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公开(公告)号：US11913007B2
公开(公告)日：2024-02-27
申请号：US17553013
申请日：2021-12-16
发明人： Jörg Bauer , Johnathan A. Napier , Olga Sayanova
IPC分类号： C07H21/04 , A01H5/00 , C07K14/405 , C12N15/82 , C07K14/435 , C12P7/6434 , C12P7/6427 , C12P7/6472 , C12P7/6432 , C12N9/02 , C12N9/00
CPC分类号： C12N15/8247 , A01H5/00 , C07H21/04 , C07K14/405 , C07K14/435 , C12N9/0071 , C12N9/93 , C12P7/6427 , C12P7/6432 , C12P7/6434 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y114/19004 , C12Y114/19006 , C12Y602/01003
摘要： The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturases and elongases from the organism Emiliana huxleyi. The invention also provides recombinant expression vectors containing desaturase or elongase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g. arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).
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公开(公告)号：US20240150781A1
公开(公告)日：2024-05-09
申请号：US18409598
申请日：2024-01-10
申请人： Commonwealth Scientific and Industrial Research Organisation , GRAINS RESEARCH AND DEVELOPMENT CORPORATION
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
IPC分类号： C12N15/82 , C12P7/6427 , C12P7/6432 , C12P7/6434 , C12P7/6472
CPC分类号： C12N15/8247 , C12P7/6427 , C12P7/6432 , C12P7/6434 , C12P7/6472 , C12Y602/01003
摘要： The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
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公开(公告)号：US20230416792A1
公开(公告)日：2023-12-28
申请号：US18220975
申请日：2023-07-12
IPC分类号： C12P7/6427 , C12P7/6463 , C12P7/6472 , C12P7/6434 , C12P7/6458 , C11B1/10
CPC分类号： C12P7/6427 , C12P7/6463 , C11B1/10 , C12P7/6434 , C12P7/6458 , C12P7/6472
摘要： Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.
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公开(公告)号：US20240150782A1
公开(公告)日：2024-05-09
申请号：US18409603
申请日：2024-01-10
申请人： Commonwealth Scientific and Industrial Research Organisation , GRAINS RESEARCH AND DEVELOPMENT CORPORATION
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
IPC分类号： C12N15/82 , C12P7/6427 , C12P7/6432 , C12P7/6434 , C12P7/6472
CPC分类号： C12N15/8247 , C12P7/6427 , C12P7/6432 , C12P7/6434 , C12P7/6472 , C12Y602/01003
摘要： The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 desaturases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
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5.发明公开公开(公告)号：US20240101953A1
公开(公告)日：2024-03-28
申请号：US18255943
申请日：2021-11-08
发明人： Jung Woon CHOI , Sung Hoon JANG , Ji Young KIM , Won Sub SHIN , Hae Won KANG
IPC分类号： C12N1/12 , A23K10/16 , C12P7/6409 , C12P7/6432 , C12P7/6434
CPC分类号： C12N1/125 , A23K10/16 , C12P7/6409 , C12P7/6432 , C12P7/6434 , C12R2001/89
摘要： The present application relates to a novel strain of the genus Schizochytrium (Schizochytrium sp.) and a method of producing polyunsaturated fatty acids by using the same. According to one aspect, novel microalgae of the genus Schizochytrium have a high content of fat in biomass, and particularly, a high content of polyunsaturated fatty acids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), and therefore, the microalgae, and biomass or bio-oil prepared therefrom, may be useful as a feed source or the like.
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公开(公告)号：US11840714B2
公开(公告)日：2023-12-12
申请号：US17273140
申请日：2019-09-04
申请人： Nissui Corporation
IPC分类号： C12P7/6434 , C12P7/6472 , C12P7/6427 , C12P7/6432
CPC分类号： C12P7/6434 , C12P7/6427 , C12P7/6432 , C12P7/6472
摘要： The present invention provides a method of producing a composition containing docosahexaenoic acid as a constituent fatty acid of glycerides. The method includes hydrolyzing a feedstock oil containing glycerides, the glycerides including docosahexaenoic acid as a constituent fatty acid, by action of a first lipase and a second lipase, to increase the proportion of docosahexaenoic acid in glyceride fractions. The first lipase is at least one lipase selected from the group consisting of a lipase obtained from a microorganism of the genus Thermomyces, a lipase obtained from a microorganism of the genus Pseudomonas, a lipase obtained from a microorganism of the genus Burkholderia, and a lipase obtained from a microorganism of the genus Alcaligenes. The second lipase is a partial glyceride lipase.
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公开(公告)号：US20240327883A1
公开(公告)日：2024-10-03
申请号：US18600576
申请日：2024-03-08
发明人： Jeong Woo SEO , Jong Jae YI , Sun Yeon HEO , Young Bae KIM , Chul Ho KIM , Baek Rock OH , Jung Hyun JU , Hack Sun CHOI
IPC分类号： C12P7/6427 , A61P35/00 , C11C3/00 , C12N9/02 , C12P7/6432 , C12P7/6434 , C12P17/02
CPC分类号： C12P7/6427 , A61P35/00 , C11C3/00 , C12N9/0069 , C12P7/6432 , C12P7/6434 , C12P17/02
摘要： The present invention relates to a novel enzyme capable of producing multi-hydroxy derivatives from polyunsaturated fatty acids and a method for producing multi-hydroxy derivatives of polyunsaturated fatty acids using the same.
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公开(公告)号：US20240150779A1
公开(公告)日：2024-05-09
申请号：US18409591
申请日：2024-01-10
申请人： Commonwealth Scientific and Industrial Research Organisation , GRAINS RESEARCH AND DEVELOPMENT CORPORATION
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
IPC分类号： C12N15/82 , C12P7/6427 , C12P7/6432 , C12P7/6434 , C12P7/6472
CPC分类号： C12N15/8247 , C12P7/6427 , C12P7/6432 , C12P7/6434 , C12P7/6472 , C12Y602/01003
摘要： The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
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公开(公告)号：US20240026394A1
公开(公告)日：2024-01-25
申请号：US18265094
申请日：2021-11-19
发明人： Rima JABER , Katharina BERSE , Eva Maria WITTMANN
IPC分类号： C12P7/6434
CPC分类号： C12P7/6434
摘要： The present invention refers to a method for producing hydroxylated fatty acids by oxidizing at least one unsaturated fatty acid by at least one lipoxygenase and thereafter reducing the obtained compound by at least one peroxidase and/or heating. Furthermore, the present invention refers to the compound obtained by said method.
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公开(公告)号：US20240360464A1
公开(公告)日：2024-10-31
申请号：US18656570
申请日：2024-05-06
申请人： Commonwealth Scientific and Industrial Research Organisation , GRAINS RESEARCH AND DEVELOPMENT CORPORATION
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
IPC分类号： C12N15/82 , C12P7/6427 , C12P7/6432 , C12P7/6434 , C12P7/6472
CPC分类号： C12N15/8247 , C12P7/6427 , C12P7/6432 , C12P7/6434 , C12P7/6472 , C12Y602/01003
摘要： The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides Ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
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