公开(公告)号：US20240131145A1

公开(公告)日：2024-04-25

申请号：US18276905

申请日：2022-02-16

申请人： Children's Hospital Medical Center

发明人： Ming TAN ,  Xi Jason JIANG

IPC分类号： A61K39/145 ,  A61P31/16 ,  C07K14/005 ,  C12N7/00

CPC分类号： A61K39/145 ,  A61P31/16 ,  C07K14/005 ,  C12N7/00 ,  A61K2039/545 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16222 ,  C12N2760/16223 ,  C12N2760/16234 ,  C12N2770/16022 ,  C12N2770/16023 ,  C12N2770/16042

摘要： The instant disclosure relates to pseudovirus nanoparticles (PVNPs) and compositions comprising PVNPs. The disclosed PVNPs may be comprised of fusion proteins that form an icosahedral structure and a nanoparticle shell. The disclosed fusion proteins may comprise a modified norovirus (NoV) S domain protein; a hemagglutinin I (HA1) antigen of the influenza hemagglutinin I (HA1) of influenza vims; and a peptide linker connecting the C-terminus of the NoV S domain to the HA1 antigen. The modified NoV S domain proteins form the interior nanoparticle shell of said PVNP composition and display the 60 HA1 antigens on the surface of the nanoparticle shell. Methods of making and using the PVNPs and compositions containing PVNPs are also disclosed.

公开(公告)号：US11865174B2

公开(公告)日：2024-01-09

申请号：US17367296

申请日：2021-07-02

申请人： Hugh Mason ,  Andrew Diamos ,  Mary Pardhe ,  Brandon Favre

发明人： Hugh Mason ,  Andrew Diamos ,  Mary Pardhe ,  Brandon Favre

IPC分类号： A61K39/12 ,  A61K39/29 ,  A61K39/00 ,  A61K39/295 ,  A61P31/20 ,  C12N7/00 ,  A61K39/145

CPC分类号： A61K39/292 ,  A61K39/12 ,  A61K39/145 ,  A61P31/20 ,  C12N7/00 ,  A61K2039/505 ,  A61K2039/5258 ,  A61K2039/575 ,  A61K2039/6056 ,  A61K2039/70 ,  C12N2710/20023 ,  C12N2710/20034 ,  C12N2710/20071 ,  C12N2730/10123 ,  C12N2730/10134 ,  C12N2730/10171 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16171 ,  C12N2770/24134 ,  C12N2770/24171

摘要： The disclosure relates to vaccination compositions, for example, against human papillomavirus, Zika virus, and flu virus. The disclosure also relates to vectors for producing the virus-like particles and immune complex platforms of the vaccination compositions.

公开(公告)号：US11833200B2

公开(公告)日：2023-12-05

申请号：US13497767

申请日：2010-09-21

申请人： Louis-Philippe Vezina ,  Manon Couture ,  Dany Paquet ,  Michele Dargis ,  Marc-Andre D'Aoust

发明人： Louis-Philippe Vezina ,  Manon Couture ,  Dany Paquet ,  Michele Dargis ,  Marc-Andre D'Aoust

IPC分类号： C12N7/00 ,  C12N15/82 ,  A61K39/145 ,  A61K39/12 ,  A61K39/00

CPC分类号： A61K39/145 ,  A61K39/12 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  A61K2039/517 ,  A61K2039/5258 ,  C12N2760/16123 ,  C12N2760/16134

摘要： Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.

公开(公告)号：US11826419B2

公开(公告)日：2023-11-28

申请号：US13497757

申请日：2010-09-21

申请人： Louis-Philippe Vezina ,  Manon Couture ,  Dany Paquet ,  Michele Dargis ,  Marc-Andre D'Aoust

发明人： Louis-Philippe Vezina ,  Manon Couture ,  Dany Paquet ,  Michele Dargis ,  Marc-Andre D'Aoust

IPC分类号： C12N7/00 ,  C12N15/82 ,  A61K39/145 ,  A61K39/12 ,  A61K39/00

CPC分类号： A61K39/145 ,  A61K39/12 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  A61K2039/517 ,  A61K2039/5258 ,  C12N2760/16123 ,  C12N2760/16134

摘要： Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.

公开(公告)号：US20230201336A1

公开(公告)日：2023-06-29

申请号：US17996300

申请日：2021-03-09

申请人： ÖSTERREICHISCHE AGENTUR FÜR GESUNDHEIT UND ERNÄHRUNGSSICHERHEIT GMBH

发明人： Boris HARTMANN

IPC分类号： A61K39/295 ,  A61K39/145 ,  C07K14/005

CPC分类号： A61K39/295 ,  A61K39/145 ,  C07K14/005 ,  C12N2760/16123 ,  C12N2760/16134 ,  A61K2039/543

摘要： Disclosed are influenza



virus-like particles (VLPs), wherein the VLPs
include hemagglutinin (HA) protein and neuraminidase (NA) protein on the surface of the VLPs,
a nucleoprotein (NP) ribonucleoprotein complex, and
wherein the VLPs do not contain a ribonucleoprotein complex of at least one of PB1, PB2, and NS.

公开(公告)号：US09968670B2

公开(公告)日：2018-05-15

申请号：US14652643

申请日：2013-12-17

申请人： Icahn School of Medicine at Mount Sinai

发明人： Adolfo Garcia-Sastre ,  Peter Palese ,  Florian Krammer

IPC分类号： A61K39/145 ,  C12N7/00 ,  C07K14/005 ,  A61K39/12 ,  A61K39/00

CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/521 ,  A61K2039/5252 ,  A61K2039/54 ,  A61K2039/543 ,  A61K2039/545 ,  A61K2039/55 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16223 ,  C12N2760/16234

摘要： Provided herein are chimeric influenza hemagglutinin (HA) polypeptides, compositions comprising the same, vaccines comprising the same, and methods of their use.

公开(公告)号：US09580475B2

公开(公告)日：2017-02-28

申请号：US14126550

申请日：2012-06-20

申请人： Ted M. Ross ,  Corey J. Crevar

发明人： Ted M. Ross ,  Corey J. Crevar

IPC分类号： C07K14/005 ,  A61K39/145 ,  A61K39/12 ,  A61K39/00

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5258 ,  A61K2039/55505 ,  C12N7/00 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16134

摘要： Described herein is the generation of optimized H1N1 influenza HA polypeptides for eliciting a broadly reactive immune response to H1N1 influenza virus isolates. The optimized HA polypeptides were developed through a series of HA protein alignments, and subsequent generation of consensus sequences, based on selected H1N1 viruses isolated from 1918-2011. Provided herein are optimized H1N1 HA polypeptides, and compositions, fusion proteins and VLPs comprising the HA polypeptides. Further provided are codon-optimized nucleic acid sequences encoding the HA polypeptides. Methods of eliciting an immune response against influenza virus in a subject are also provided by the present disclosure.

摘要翻译： 本文描述的是产生用于引发对H1N1流感病毒分离物的广泛反应性免疫应答的优化的H1N1流感HA多肽。 基于从1918-2011分离的所选择的H1N1病毒，通过一系列HA蛋白质比对和随后产生的共有序列来开发优化的HA多肽。 本文提供了优化的H1N1HA多肽，以及包含HA多肽的组合物，融合蛋白和VLP。 还提供了编码HA多肽的密码子优化的核酸序列。 在本公开内容中也提供了在受试者中引起针对流感病毒的免疫应答的方法。

公开(公告)号：US20160348144A1

公开(公告)日：2016-12-01

申请号：US15164968

申请日：2016-05-26

申请人： Academia Sinica

发明人： Chi-Huey Wong ,  Che Ma ,  Yung-Chieh Tseng

IPC分类号： C12P21/00 ,  C12N7/00

CPC分类号： C12P21/005 ,  A61K39/12 ,  A61K39/145 ,  C07K14/005 ,  C12N7/00 ,  C12N2760/16111 ,  C12N2760/16121 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12Y302/01096 ,  G01N2333/00 ,  G01N2333/11 ,  Y02A50/386 ,  Y02A50/394 ,  Y02P20/52

摘要： Methods for production of virus particles with simplified glycosylation on structural or surface proteins are provided. When used as targets for vaccine production, the conserved nature of such sites generates vaccines that are less sensitive to viral mutations. Use of glycosylation inhibitors for production of viruses with simplified glycosylation profiles are disclosed. An exemplary disclosure of influenza viruses and methods for production of mono-glycosylated influenza virus particles is provided. Methods for production of mono-glycosylated forms of influenza A virus, NIBRG-14 (H5N1) are provided.

摘要翻译： 提供了在结构或表面蛋白上生成具有简化糖基化的病毒颗粒的方法。 当用作疫苗生产的目标时，这些位点的保守性质产生对病毒突变较不敏感的疫苗。 公开了糖基化抑制剂用于产生具有简化糖基化特征的病毒的用途。 提供流感病毒的示例性公开和用于生产单糖基化流感病毒颗粒的方法。 提供了甲型流感病毒，NIBRG-14（H5N1）单糖基化形式的生产方法。

公开(公告)号：US09434959B2

公开(公告)日：2016-09-06

申请号：US14249864

申请日：2014-04-10

申请人： BEN GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT AUTHORITY

发明人： Leslie Lobel ,  Guy Gubi

IPC分类号： A01N63/00 ,  A61K38/00 ,  A61K39/395 ,  C07H21/04 ,  C07K1/00 ,  C12N15/86 ,  A61K39/145 ,  C07K14/005 ,  C07K16/10 ,  C12N7/00 ,  A61K39/12 ,  C12N7/02 ,  C12N7/04 ,  A61K39/00

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5258 ,  C07H21/04 ,  C07K14/005 ,  C07K16/1018 ,  C07K2317/622 ,  C07K2317/80 ,  C12N7/00 ,  C12N7/02 ,  C12N7/045 ,  C12N2760/14134 ,  C12N2760/16122 ,  C12N2760/16123 ,  C12N2760/16143

摘要： The invention provides virus-like particles for treatment of viral infections based on the virus causing the infection. The virus-like particles comprise the virus recombinant proteins that form a capsid, recombinant virus membrane proteins attached to the capsid and vRNA packaged within said capsid. The vRNA is generated from a DNA sequence encoding a polypeptide capable of specifically binding to a constant region of a nonstructural protein of the virus that is essential for propagation of the virus.

摘要翻译： 本发明提供病毒样颗粒，用于治疗基于导致感染的病毒的病毒感染。 病毒样颗粒包含形成衣壳衣壳重组病毒膜蛋白的病毒重组蛋白，所述重组病毒膜蛋白与衣壳连接，并且包裹在所述衣壳内的vRNA。 该vRNA由编码能够特异性结合该病毒的非结构蛋白质的非结构蛋白质的恒定区的多肽的DNA序列产生，该结构蛋白对病毒的繁殖是必需的。

公开(公告)号：US09309536B2

公开(公告)日：2016-04-12

申请号：US14472911

申请日：2014-08-29

申请人： Pfizer Inc.

发明人： Corinne John ,  Christian Schaub ,  Sabine Wellnitz

IPC分类号： C12N15/86 ,  C12N7/00 ,  A61K39/00

CPC分类号： C12N15/86 ,  A61K2039/5256 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2710/14043 ,  C12N2710/14143 ,  C12N2710/16123 ,  C12N2710/20023 ,  C12N2710/20034 ,  C12N2760/16023 ,  C12N2760/16123 ,  C12N2760/16134

摘要： The invention describes novel virus-like particles for use as vaccines, diagnostic tools and R&D tools based on recombinant DNA and cell cultivation techniques for production. The recombinant virus-like particles of the invention are assembled by polypeptide chains that incorporate several, in particular two or more, different epitopes which are selected either (a) from different viral strains of the same virus and/or (b) from different serotypes of the same virus and/or (c) from different viral strains specific for different hosts. These epitopes are then displayed on the particle surface.

摘要翻译： 本发明描述了用作疫苗，诊断工具和基于重组DNA和用于生产的细胞培养技术的研发工具的新型病毒样颗粒。 本发明的重组病毒样颗粒通过多肽链组装，其包含数个，特别是两个或多个不同的表位，其选自（a）来自相同病毒的不同病毒株和/或（b）不同血清型 相同病毒和/或（c）来自不同宿主特异性的不同病毒株。 然后将这些表位显示在颗粒表面上。