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    Animal model of and test for Alzheimer's disease
    3.
    发明申请
    Animal model of and test for Alzheimer's disease 审中-公开
    阿尔茨海默病的动物模型和测试

    公开(公告)号：US20030059797A1

    公开(公告)日：2003-03-27

    申请号：US10126467

    申请日：2002-04-19

    发明人： Elena Paley

    IPC分类号： C12N005/00 ,  C12N005/02 ,  C12Q001/68 ,  G01N033/53

    CPC分类号： C12N5/0618 ,  C12N2500/46 ,  C12N2501/825 ,  C12N2503/00

    摘要： Tryptamine, a normal constituent of the diet and an inhibitor of tryptophanyl-tRNA synthetase (TrpRS), induces formation of Alzheimer's disease characteristics as extracellular senile plaques and neurofibrillary tangles containing paired helical neurofilaments and/or straight neurofilaments in human differentiated neuronal cells. The effect of tryptamine on neuronal cells is dose-dependent and multi-staged resulting in differentiation and subsequently axon growth, followed by neurodegeneration. TrpRS was found normally in the neuronal body, axons and dendrites, whereas tryptamine induces it in the cytoskeleton. In tryptamine-treated cells, TrpRS co-localized with abnormally phosphorylated tau in neurofibrillary tangles and with null-amyloid in senile plaque-like formations. Moreover, TrpRS was detected in neurofibrillary tangles and congophilic senile plaques in Alzheimer's disease brain sections and brain sections of model mice following tryptamine treatment. The secreted TrpRS was demonstrated in human sera. These findings provide a new perspective on the versatility of TrpRS functions and indicate that tryptamine may be involved in the induction of Alzheimer's disease.

    摘要翻译： 膳食中正常成分的色胺和色氨酰-tRNA合成酶（TrpRS）的抑制剂诱导阿尔茨海默病特征的形成，作为在分化型神经元细胞中含有成对螺旋神经丝和/或直接神经丝的细胞外老化斑和神经原纤维缠结。 色胺对神经细胞的影响是剂量依赖性和多阶段的，导致分化和随后的轴突生长，随后是神经变性。 TrpRS通常在神经元体，轴突和树突中发现，而色胺在细胞骨架中诱导。 在色胺处理的细胞中，TrpRS与神经原纤维缠结中的异常磷酸化tau共同定位，并在老年斑块状结构中与β-淀粉样蛋白共定位。 此外，在色胺处理后，在阿尔茨海默病脑切片和模型小鼠的脑切片中，在神经原纤维缠结和同位素老年斑中检测到TrpRS。 人血清中证明了分泌的TrpRS。 这些发现为TrpRS功能的多功能性提供了新的视角，并表明色胺可能参与阿尔茨海默病的诱导。

    Neuronal stimulation
    5.
    发明授权
    Neuronal stimulation 有权
    神经元刺激

    公开(公告)号：US08790889B2

    公开(公告)日：2014-07-29

    申请号：US13518234

    申请日：2010-12-21

    申请人： Dhanisha Jhaveri ,  Perry Francis Bartlett

    发明人： Dhanisha Jhaveri ,  Perry Francis Bartlett

    IPC分类号： C12Q1/02 ,  C12N5/00

    CPC分类号： A61K35/30 ,  C12N5/0623 ,  C12N2501/80 ,  C12N2501/825

    摘要： A method of activating a latent neural precursor cell population, comprising: (1) providing a neural cell population derived from the hippocampus; (2) introducing the neural cell population to a neurosphere-forming culture medium; and (3) activating the latent precursor cell population by treatment with a β3 adrenergic receptor agonist.

    摘要翻译： 一种激活潜在神经前体细胞群的方法，包括：（1）提供衍生自海马的神经细胞群; （2）将神经细胞群导入神经球形成培养基中; 和（3）通过用β-肾上腺素能受体激动剂治疗来激活潜在前体细胞群体。

    MEDIUM FOR MAMMALIAN SOMATIC CELLS AND ADDITIVE THEREFOR
    7.
    发明申请
    MEDIUM FOR MAMMALIAN SOMATIC CELLS AND ADDITIVE THEREFOR 审中-公开
    MAMMALIAN SOMATIC CELLS的添加剂及其添加剂

    公开(公告)号：US20110008893A1

    公开(公告)日：2011-01-13

    申请号：US12810729

    申请日：2008-12-26

    申请人： Itsuro Sugimura ,  Yoshiyuki Hotta ,  Harumi Yamaguma

    发明人： Itsuro Sugimura ,  Yoshiyuki Hotta ,  Harumi Yamaguma

    IPC分类号： C12N5/071 ,  C12N5/02

    CPC分类号： C12N5/0663 ,  C12N5/0667 ,  C12N2500/36 ,  C12N2501/825

    摘要： Disclosed are a medium for mammalian somatic cells with which mammalian somatic cells can be grown effectively when the mammalian somatic cells are cultured, while reducing the amount of serum to be added to the medium as much as possible or without adding serum thereto, and an additive to constitute the medium. By blending of a ligand for an endothelial cell differentiation gene (Edg) family receptor and a ligand for a serotonin receptor to a medium, somatic cells of mammals can be grown even in cases where the medium does not contain serum at all or contains only a small amount thereof.

    摘要翻译： 公开了哺乳动物体细胞的培养基，哺乳动物体细胞可以在哺乳动物体细胞培养时有效生长，同时尽可能减少添加到培养基中的血清量或不添加血清，添加剂 构成媒介。 通过将内皮细胞分化基因（Edg）家族受体的配体和血清素受体的配体混合到培养基中，哺乳动物的体细胞即使在培养基完全不含血清或只含有 少量。

    CULTURE MEDIUM FOR PRODUCING EXTRACELLULAR VESICLES, CULTURE MEDIUM KIT, ADDITIVE, AND METHOD FOR PRODUCING EXTRACELLULAR VESICLES
    9.
    发明公开
    CULTURE MEDIUM FOR PRODUCING EXTRACELLULAR VESICLES, CULTURE MEDIUM KIT, ADDITIVE, AND METHOD FOR PRODUCING EXTRACELLULAR VESICLES 审中-公开

    公开(公告)号：US20230250397A1

    公开(公告)日：2023-08-10

    申请号：US18300741

    申请日：2023-04-14

    申请人： FUJIFILM Corporation

    发明人： Masayuki YAMANE ,  Hanako ARAI

    IPC分类号： C12N5/0775

    CPC分类号： C12N5/0663 ,  C12N2500/32 ,  C12N2501/33 ,  C12N2501/105 ,  C12N2501/825 ,  C12N2501/15 ,  C12N2500/24

    摘要： An object of the present invention is to provide a culture medium for producing extracellular vesicles, a culture medium kit, an additive, and a method for producing extracellular vesicles, whereby the production of extracellular vesicles is promoted. The present invention is to provide a culture medium for producing extracellular vesicles. The culture medium for producing extracellular vesicles contains a basal medium for culturing animal cells, and at least one component selected from L-glutamine or a salt thereof, transferrin, selenious acid or a salt thereof, insulin, insulin-like growth factors, a serotonin compound, and transforming growth factor β. Furthermore, in a case of containing L-glutamine or a salt thereof, the culture medium for producing extracellular vesicles contains L-glutamine or a salt thereof with a concentration of 5 mM or more in the culture medium for producing extracellular vesicles. The present invention is to further provide a culture medium kit, an additive, and a method for producing extracellular vesicles.