MODERATION OF CRUSTACEAN ANDROGENIC GLAND PEPTIDE FOR MONO SEX CULTURE
    2.
    发明申请
    MODERATION OF CRUSTACEAN ANDROGENIC GLAND PEPTIDE FOR MONO SEX CULTURE 审中-公开
    用于单性生殖培养的胃肠球蛋白肽的研究

    公开(公告)号:US20170035034A1

    公开(公告)日:2017-02-09

    申请号:US15236356

    申请日:2016-08-12

    申请人: Siu Ming Chan

    发明人: Siu Ming Chan

    IPC分类号: A01K61/00 C12N15/113

    摘要: This invention is directed to methods for manipulating sex, reversing sex, changing sex ratio, or moderating crustacean androgenic gland peptide expression in crustaceans for producing a monosex culture. In one embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a male crustacean. In another embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a female crustacean that had acquired a spermatophore (a sperm sac) after mating with a male. In another embodiment, the present invention provides a method of producing a monosex culture of crustacean by introducing a recombinant IAG peptide into the female crustacean, thereby obtains male with altered sexual characteristics for mating with a normal female. In one embodiment, the crustacean is a shrimp or lobster.

    摘要翻译: 本发明涉及用于操纵性别,逆转性别,改变性别比例或调节甲壳类动物中甲壳动物腺体肽表达以产生单调性培养物的方法。 在一个实施方案中,本方法包括将IAG基因的dsRNA注入雄性甲壳动物的步骤。 在另一个实施方案中,本方法包括将IAG基因的dsRNA注射到与雄性交配后获得精子细胞(精子囊)的雌性甲壳动物的步骤。 在另一个实施方案中,本发明提供了通过将重组IAG肽引入雌性甲壳动物来生产甲壳类动物单次培养物的方法,从而获得具有改变的性状特征的雄性与正常雌性交配。 在一个实施方案中,甲壳类是虾或龙虾。

    EXPRESSION SYSTEMS
    3.
    发明申请
    EXPRESSION SYSTEMS 审中-公开
    表达系统

    公开(公告)号:US20160060651A1

    公开(公告)日:2016-03-03

    申请号:US14839683

    申请日:2015-08-28

    申请人: Oxitec Limited

    发明人: Luke ALPHEY

    IPC分类号: C12N15/85

    摘要: A gene expression system is provided. The system comprises at least one coding sequence to be expressed in an organism, and at least one promoter operably linked thereto. It further comprises at least one splice control sequence which, in cooperation with a spliceosome, mediates alternative splicing of RNA transcripts of the coding sequence. The mediation of alternative splicing is in a sex-specific, stage-specific, germline-specific and tissue-specific manner.

    摘要翻译: 提供了基因表达系统。 该系统包含至少一个要在生物体中表达的编码序列,以及至少一个与其可操作地连接的启动子。 它还包括至少一个剪接控制序列,其与剪接体协同介导编码序列的RNA转录物的可变剪接。 选择性剪接的调解是以性别特异性,阶段特异性,种系特异性和组织特异性方式。

    Expression systems
    4.
    发明授权
    Expression systems 有权
    表达系统

    公开(公告)号:US09133477B2

    公开(公告)日:2015-09-15

    申请号:US11352177

    申请日:2006-02-10

    申请人: Luke Alphey

    发明人: Luke Alphey

    摘要: A gene expression system is provided. The system comprises at least one coding sequence to be expressed in an organism, and at least one promoter operably linked thereto. It further comprises at least one splice control sequence which, in cooperation with a spliceosome, mediates alternative splicing of RNA transcripts of the coding sequence. The mediation of alternative splicing is in a sex-specific, stage-specific, germline-specific and tissue-specific manner.

    摘要翻译: 提供了基因表达系统。 该系统包含至少一个要在生物体中表达的编码序列,以及至少一个与其可操作地连接的启动子。 它还包括至少一个剪接控制序列,其与剪接体协同介导编码序列的RNA转录物的可变剪接。 选择性剪接的调解是以性别特异性,阶段特异性,种系特异性和组织特异性方式。

    Nucleotide sequence of shrimp actin promoter and its use in genetic transformation biotechnology
    7.
    发明授权
    Nucleotide sequence of shrimp actin promoter and its use in genetic transformation biotechnology 有权
    虾肌动蛋白启动子的核苷酸序列及其在遗传转化生物技术中的应用

    公开(公告)号:US08242253B2

    公开(公告)日:2012-08-14

    申请号:US12274627

    申请日:2008-11-20

    摘要: The present invention relates to an isolated β-actin nucleic acid promoter molecule from shrimp; nucleic acid constructs including the β-actin promoter molecule; and expression vectors, host cells, and transgenic animals transduced with the isolated β-actin nucleic acid promoter. Also disclosed are methods for imparting to an animal resistance against a pathogen, regulating growth of an animal, increasing stress tolerance in an animal, and increasing cold tolerance in an animal that involves transforming an animal with a nucleic acid construct including the isolated β-actin nucleic acid promoter molecule of the present invention.

    摘要翻译: 本发明涉及来自虾的分离的β-肌动蛋白核酸启动子分子; 核酸构建体,包括β-肌动蛋白启动子分子; 和表达载体,宿主细胞和用分离的β-肌动蛋白核酸启动子转导的转基因动物。 还公开了赋予动物对病原体抗性,调节动物生长,增加动物中的胁迫耐受性以及增加动物中抗寒性的方法,所述动物涉及用包含分离的 - 肌动蛋白核酸启动子分子。

    INSULIN-LIKE GENE IN PRAWNS AND USES THEREOF
    9.
    发明申请
    INSULIN-LIKE GENE IN PRAWNS AND USES THEREOF 有权
    胰岛素样基因在其中的用途及其用途

    公开(公告)号:US20120073508A1

    公开(公告)日:2012-03-29

    申请号:US13277088

    申请日:2011-10-19

    摘要: The present invention relates to a nucleic acid molecule encoding an insulin-like factor of the androgenic gland of the freshwater prawn Macrobrachium rosenbergii (M. rosenbergii). The present invention further relates to methods of silencing the expression of the insulin-like factor gene in decapod crustaceans order, particularly in M. rosenbergii, useful for producing male monosex populations.

    摘要翻译: 本发明涉及编码淡水虾罗氏沼虾(Rosenbergii)的雄激素腺的胰岛素样因子的核酸分子。 本发明进一步涉及使十字头甲壳类动物中特别是罗氏沼虾中的胰岛素样因子基因的表达沉默的方法,其可用于产生雄性单体种群。