METHODS AND KITS FOR PREPARING A SAMPLE COMPRISING ONE OR MORE RNA MOLECULES FOR SEQUENCING

    公开(公告)号:US20240309447A1

    公开(公告)日:2024-09-19

    申请号:US18601981

    申请日:2024-03-11

    IPC分类号: C12Q1/6874 C12Q1/6806

    摘要: One example embodiment is a method for preparing a sample including one or more RNA molecules, including the steps of: (i) reacting the sample with a reaction mixture including a poly(A) polymerase and one or more predefined nucleotide analogues to add a first sequence including one or more predefined nucleotide analogues to individual 3′-end of the one or more RNA molecules, such that one or more first tailed RNA is formed; and (ii) adding a plurality of adenosine triphosphates (ATP) to the reaction mixture, such that the first tailed RNA is reacted with a plurality of adenosine triphosphates (ATP) to add a second sequence including a plurality of adenine nucleotides to individual 3′-end of the first tailed RNA, such that one or more second tailed RNA is formed. Other example embodiments are described herein. In certain embodiments, the disclosed methods and kits are quick, simple and easy to operate.

    Multifunctional and modular geotechnical testing device

    公开(公告)号:US12000820B2

    公开(公告)日:2024-06-04

    申请号:US17248559

    申请日:2021-01-29

    IPC分类号: G01N33/24 G01N1/08 G01N1/10

    摘要: A multifunctional and modular geotechnical testing device for testing a soil sample is provided. The testing device includes a testing cell, a plurality of needle probes, and a plurality of lateral pressure-measuring sensors. The testing cell has an oedometer ring for accommodating the soil sample, with a plurality of holes located on and angularly distributed over an internal lateral surface of the oedometer ring forming a plurality of channels to access the soil sample from outside. The plurality of needle probes, each configured to house a pore water pressure-measuring sensor, is detachably mountable to the plurality of channels from outside for simultaneously measuring the pore water pressures. The plurality of lateral pressure-measuring sensors is removably attachable to an inner peripheral side of the testing cell for measuring lateral pressures of the soil sample.

    JOINT COUNT AND FLOW ANALYSIS FOR VIDEO CROWD SCENES

    公开(公告)号:US20240144490A1

    公开(公告)日:2024-05-02

    申请号:US18220077

    申请日:2023-07-10

    IPC分类号: G06T7/269 G06T7/246

    摘要: A system for flow-count includes one or more cameras and a processing system. The one more cameras are configured to capture image data comprising a sequence of frames. The processing system is configured to extract spatial features and temporal features based on the sequence of frames; construct, based on the spatial features and temporal features, a density map and/or a flow map corresponding to a frame of the sequence of frames, wherein the density map comprises density values for the plurality of pixels, wherein the flow map comprises two-dimensional (2D) vectors for the plurality of pixels, and wherein a 2D vector indicates a speed and direction for a pixel of the plurality of pixels in the frame; and determine, based on the density map and/or the flow map, a count, a distribution, and/or movement of objects corresponding to the frame.