公开(公告)号：US20240309447A1

公开(公告)日：2024-09-19

申请号：US18601981

申请日：2024-03-11

申请人： The Hong Kong University of Science and Technology

发明人： Yi KUANG ,  Zhenghua LIANG ,  Angela Ruohao WU

IPC分类号： C12Q1/6874 ,  C12Q1/6806

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q2600/158

摘要： One example embodiment is a method for preparing a sample including one or more RNA molecules, including the steps of: (i) reacting the sample with a reaction mixture including a poly(A) polymerase and one or more predefined nucleotide analogues to add a first sequence including one or more predefined nucleotide analogues to individual 3′-end of the one or more RNA molecules, such that one or more first tailed RNA is formed; and (ii) adding a plurality of adenosine triphosphates (ATP) to the reaction mixture, such that the first tailed RNA is reacted with a plurality of adenosine triphosphates (ATP) to add a second sequence including a plurality of adenine nucleotides to individual 3′-end of the first tailed RNA, such that one or more second tailed RNA is formed. Other example embodiments are described herein. In certain embodiments, the disclosed methods and kits are quick, simple and easy to operate.