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公开(公告)号：US5443965A
公开(公告)日：1995-08-22
申请号：US681703
申请日：1991-04-05
IPC分类号： A61K39/00 , A61K38/00 , A61K39/29 , C07K14/00 , C07K14/02 , C07K14/10 , C07K14/18 , C12N1/21 , C12N15/09 , C12N15/51 , C12P21/02 , C12Q1/70 , C12R1/19 , G01N33/15 , G01N33/50 , G01N33/53 , G01N33/576 , C12P21/06 , C07H17/00
CPC分类号： C07K14/005 , A61K38/00 , C12N2770/24222 , Y10S530/826
摘要： Peptide antigens which are immunoreactive with sera from individuals infected with hepatitis C virus (HCV) are disclosed. Several of the antigens are immunologically reactive with antibodies present in individuals identified as having chronic and acute HCV infection. The antigens are useful in diagnostic methods for detecting HCV infection in humans. Also disclosed are corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic peptides.
摘要翻译： 公开了与丙型肝炎病毒（HCV）感染的个体的血清免疫反应的肽抗原。 几种抗原与被鉴定为具有慢性和急性HCV感染的个体中存在的抗体具有免疫反应性。 抗原可用于检测人类HCV感染的诊断方法。 还公开了含有编码抗原肽的开放阅读框序列的多核苷酸的相应基因组片段克隆。
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公开(公告)号：US5108920A
公开(公告)日：1992-04-28
申请号：US361855
申请日：1989-06-05
CPC分类号： C07K14/005 , C12N7/00 , C12Q1/702 , C12N2740/10022
摘要： A novel retrovirus isolated from human lymphoma cells is disclosed. The retrovirus is characterized by a C-type retroviral particle of approximately 100 nm diameter; and an approximately 27,000 molecular weight p24 core protein. Also disclosed are cell lines from which the virus can be obtained and screening methods for detecting the presence of the virus in human sera.
摘要翻译： 公开了从人类淋巴瘤细胞分离的新型逆转录病毒。 逆转录病毒的特征在于直径约100nm的C型逆转录病毒颗粒; 和约27,000分子量的p24核心蛋白。 还公开了可以从其获得病毒的细胞系和用于检测人血清中病毒存在的筛选方法。
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公开(公告)号：US5077390A
公开(公告)日：1991-12-31
申请号：US404328
申请日：1989-09-07
申请人： Paul S. Wu , Susan B. Wade , Raul R. Soikes
发明人： Paul S. Wu , Susan B. Wade , Raul R. Soikes
IPC分类号： A61K38/00 , C07K14/415
CPC分类号： C07K14/415 , A61K38/00
摘要： A substantially pure, hemagglutinin-free composition of trichosanthin, and a method of producing the composition is disclosed. A plant extract from Trichosanthes kirilowii is contacted with an anionic exchange resin to remove contaminating hemagglutinins, and trichosanthin is further purified from the extract by cation exchange chromatography to a purity of greater than 95%.
摘要翻译： 公开了一种基本上纯的，不含血凝素的trichosanthin组合物及其制备方法。 将来自Trichosanthes kirilowii的植物提取物与阴离子交换树脂接触以除去污染性血凝素，并且通过阳离子交换层析从提取物中进一步纯化trichosanthin，纯度大于95％。
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公开(公告)号：US4777245A
公开(公告)日：1988-10-11
申请号：US767213
申请日：1985-08-19
IPC分类号： C07K16/08 , C07K16/34 , C12N5/12 , C12N5/26 , C12N15/02 , G01N33/569 , G01N33/80 , C07K15/00 , A61K39/42 , A61K39/44 , C12N5/00
CPC分类号： C07K16/088 , C07K16/08 , C07K16/34 , C12N15/02 , C12N5/12 , C12N5/166 , G01N33/569 , G01N33/56983 , G01N33/80 , Y10S435/948 , Y10S530/864
摘要： A stable trioma cell line capable of secreting a non-human primate monoclonal antibody specific against a selected antigen. An exemplary cell line secretes chimpanzee monoclonal antibody specific against an antigen associated with hepatitis nonA/nonB infection. The cell line is produced, in the method of the invention, by isolating lymphocytes from a primate immunized with the selected antigen, and immortalizing the lymphocytes by fusion with a stable, non-antibody-secreting murine myeloma/human hybridoma cell line having selected-for human characteristics. The trioma fusion products are selected for secretion of the desired antibody, which has a variety of diagnostic and/or therapeutic uses.
摘要翻译： 能够分泌针对所选择的抗原特异性的非人灵长类动物单克隆抗体的稳定的三瘤细胞系。 一个示例性细胞系分泌针对与非肝/非B型肝炎感染相关的抗原特异性的黑猩猩单克隆抗体。 在本发明的方法中，通过从用所选择的抗原免疫的灵长类动物中分离淋巴细胞，并且通过与具有选定抗原的稳定的非抗体分泌的鼠骨髓瘤/人杂交瘤细胞系融合来使淋巴细胞永生化， 为人的特点。 选择三瘤融合产物用于分泌具有各种诊断和/或治疗用途的所需抗体。
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公开(公告)号：US5166056A
公开(公告)日：1992-11-24
申请号：US804293
申请日：1991-12-09
IPC分类号： A61K38/00 , C07K14/415 , C07K14/73 , C12N15/29
CPC分类号： C07K14/70514 , C07K14/415 , A61K38/00 , C07K2319/00 , C07K2319/32 , C07K2319/55
摘要： Disclosed are the entire coding sequence for unprocessed and mature trichosanthin from Trichosanthes kirilowii, and primers derived from this coding sequence for use in obtaining the coding sequences of ribosome inactivating proteins which have regions of amino acid sequence identical to those of trichosanthin. Also disclosed is a recombinant trichosanthin protein produced from the coding sequence, and the mature protein with amino-terminal and/or carboxy-terminal extensions.
摘要翻译： 公开了来自Trichosanthes kirilowii的未加工和成熟的trichosanthin的整个编码序列，以及用于获得具有与trichosanthin的氨基酸序列相同的氨基酸序列的核糖体灭活蛋白的编码序列的该编码序列的引物。 还公开了由编码序列产生的重组trichosanthin蛋白质和具有氨基末端和/或羧基末端延伸的成熟蛋白质。
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公开(公告)号：US5128460A
公开(公告)日：1992-07-07
申请号：US504775
申请日：1990-04-04
申请人： Michael Piatak, Jr. , Theresa P. Chow , Kirk Fry
发明人： Michael Piatak, Jr. , Theresa P. Chow , Kirk Fry
IPC分类号： A61K38/00 , C07K14/415 , C07K14/73 , C12N15/29
CPC分类号： C07K14/70514 , C07K14/415 , A61K38/00 , C07K2319/00 , C07K2319/02
摘要： Disclosed are the entire protein and nucleic acid coding sequences for unprocessed and mature trichosanthin protein from Trichosanthes kirilowii. A recombinant trichosanthin protein produced from the coding sequence, and the trichosanthin protein with amino-terminal and/or carboxy-terminal extensions are also described. Primers derived from the coding sequence are disclosed for use in obtaining the coding sequences of ribosome-inactivating-proteins which have regions of amino acid sequence identical to those of trichosanthin. Further, a multigene family of ribosome-inactivating-protein encoding genes of Trichosanthes kirilowii is also described.
摘要翻译： 公开了来自Trichosanthes kirilowii的未加工和成熟的trichosanthin蛋白的整个蛋白质和核酸编码序列。 还描述了由编码序列产生的重组trichosanthin蛋白质和具有氨基末端和/或羧基末端延伸的trichosanthin蛋白质。 公开了从编码序列衍生的引物用于获得具有与天花黄素相同的氨基酸序列区域的核糖体失活蛋白的编码序列。 此外，还描述了Trichosanthes kirilowii的核糖体灭活 - 蛋白质编码基因的多基因家族。
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公开(公告)号：US4869903A
公开(公告)日：1989-09-26
申请号：US179274
申请日：1988-04-08
IPC分类号： A61K38/00 , A61K38/16 , A61P35/00 , C07K14/415
CPC分类号： A61K38/16 , C07K14/415 , Y10S514/885
摘要： A method of inhibiting HIV replication in and cellular proliferation of HIV-infected cells. The infected cells are exposed to a single-chain ribosome inactivating protein, at a protein concentration and for an exposure period sufficient to produce a substantial reduction in (a) the level of HIV antigen or reverse transcriptase associated with the infected cells, (b) the ratio of viability of infected/uninfected T cells, and/or (c) the ratio of HIV antigen/cellular antigen in infected macrophages. The method is used to treat HIV infection in humans.
摘要翻译： 抑制艾滋病病毒感染细胞的HIV复制和细胞增殖的方法。 （a）与感染细胞相关的HIV抗原或逆转录酶水平的显着降低;（b）在感染细胞中，感染的细胞暴露于单链核糖体灭活蛋白，蛋白质浓度和暴露时间足以产生（a） 感染/未感染的T细胞的存活率和/或（c）感染的巨噬细胞中HIV抗原/细胞抗原的比例。 该方法用于治疗人类的HIV感染。
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公开(公告)号：US5218099A
公开(公告)日：1993-06-08
申请号：US372711
申请日：1989-06-28
申请人： Gregory R. Reyes , Daniel W. Bradley , Linda Rabin , Kirk Fry
发明人： Gregory R. Reyes , Daniel W. Bradley , Linda Rabin , Kirk Fry
IPC分类号： A61K38/00 , A61K39/00 , A61K39/29 , C07K14/005 , C07K14/18 , C07K16/10 , C12N5/26 , C12N7/00 , C12N15/51 , C12Q1/70 , G01N33/576
CPC分类号： G01N33/5767 , A61K39/12 , A61K39/29 , C07K14/005 , C07K16/109 , C12N5/166 , C12N7/00 , C12Q1/707 , A61K38/00 , A61K39/00 , C12N2770/24221 , C12N2770/24222 , C12N2770/24234
摘要： Purified virus particles, antigens, antibodies reactive with viral antigens, and a viral genetic material associated with non-A, non-B hepatitis are provided by the present invention. Cloned genetic material useful both in identifying intact virus particles of the invention and for use in diagnostic techniques and/or production of antigens is also disclosed.
摘要翻译： 纯化的病毒颗粒，抗原，与病毒抗原反应的抗体以及与非A非乙型肝炎相关的病毒遗传物质由本发明提供。 还公开了用于鉴定本发明的完整病毒颗粒和用于诊断技术和/或抗原产生的克隆的遗传物质。
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公开(公告)号：US5169753A
公开(公告)日：1992-12-08
申请号：US816649
申请日：1992-01-02
CPC分类号： C07K14/005 , C12N5/0694 , C12N7/00 , C12Q1/702 , C12N2740/10021 , C12N2740/10022 , Y10S435/81 , Y10S435/948 , Y10S435/975
摘要： A novel retrovirus isolated from human lymphoma cells is disclosed. The retrovirus is characterized by a C-type retroviral particle of approximately 100 nm diameter; and an approximately 27,000 molecular weight p24 core protein. Also disclosed are cell lines from which the virus can be obtained and screening methods for detecting the presence of the virus in human sera.
摘要翻译： 公开了从人类淋巴瘤细胞分离的新型逆转录病毒。 逆转录病毒的特征在于直径约100nm的C型逆转录病毒颗粒; 和约27,000分子量的p24核心蛋白。 还公开了可以从其获得病毒的细胞系和用于检测人血清中病毒存在的筛选方法。
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公开(公告)号：US5101025A
公开(公告)日：1992-03-31
申请号：US404326
申请日：1989-09-07
IPC分类号： A61K38/00 , C07K14/415 , C07K14/73 , C12N15/29
CPC分类号： C07K14/70514 , C07K14/415 , A61K38/00 , C07K2319/00 , C07K2319/32 , C07K2319/55 , Y10S435/849
摘要： Disclosed are the entire coding sequence for unprocessed and mature trichosanthin from Trichosanthes kirilowii, and primers derived from this coding sequence for use in obtaining the coding sequences of ribosome inactivating proteins which have regions of amino acid sequence identical to those of trichosanthin. Also disclosed is a recombinant trichosanthin protein produced from the coding sequence, and the mature protein with amino-terminal and/or carboxy-terminal extensions.
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