公开(公告)号：US20230160883A1

公开(公告)日：2023-05-25

申请号：US17947411

申请日：2022-09-19

申请人： Yunnan Academy of Tobacco Agricultural Sciences

发明人： Ning JIANG ,  Zhenyuan XIA ,  Xiaotong GAI ,  Zhonglong LIN ,  Canhua LU ,  Changjun HUANG ,  Junhong MA ,  Xiaohan MO ,  Wei WU ,  Chunming LIU ,  Qiuqiang HOU ,  Jian GUO

IPC分类号： G01N33/531 ,  C12N5/12 ,  G01N33/543 ,  G01N33/569

CPC分类号： G01N33/531 ,  C12N5/12 ,  G01N33/54388 ,  G01N33/56983 ,  C12N2510/02 ,  G01N2333/08

摘要： This invention provides a tomato zonate spot virus detection reagent. Optimized gene encoding N protein of TZSV is expressed and purified to obtain N protein of TZSV, and two specific hybridoma cell lines are obtained, from which specific anti-TZSV monoclonal antibody could be obtained, respectively. Test strip for detecting tomato zonate spot virus is prepared with the monoclonal antibody. It is proven by experiments that the test strip of this invention is specific for TZSV, and can realize rapid detection of TZSV in leaves and other samples, with high sensitivity and convenient operation.