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公开(公告)号：US20210189419A1
公开(公告)日：2021-06-24
申请号：US17191643
申请日：2021-03-03
发明人： Yong LIU , Changjun HUANG
IPC分类号： C12N15/82 , C07K14/415
摘要： The disclosure provides a loss-of-function gene of eIFiso4E-S. The loss-of-function gene is resistant to tobacco vein banding mosaic virus (TVBMV) and represented by the sequence of SEQ ID NO: 1.
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2.发明公开公开(公告)号：US20240049674A1
公开(公告)日：2024-02-15
申请号：US17763779
申请日：2021-11-08
发明人： Changjun HUANG , Yong LIU , Haiqin YU
IPC分类号： A01H6/82 , A01H1/04 , A01H1/00 , A01H1/02 , C12Q1/6895
CPC分类号： A01H6/823 , A01H1/045 , A01H1/126 , A01H1/02 , C12Q1/6895 , C12Q2600/13
摘要： The invention relates to the field of tobacco breeding, particularly to a tobacco plant resistant to TSWD without linkage drag and a method for breeding the same. Provided is a tobacco plant or germplasm resistant to TSWD, which comprises a short RTSW introgressed segment, wherein at least part or entire of the sequence set forth in SEQ ID No. 34 is deleted in the short RTSW introgressed segment as compared to the RTSW introgressed segment of tobacco ‘Polata’. Also provided is a method for screening said tobacco plant or germplasm, in which a tobacco plant or germplasm resistant to TSWD carrying a short RTSW introgressed segment is obtained by detecting NaChr4_2M, NaChr4_8M, NaChr3_62.6M and NaChr3_64.6M linkage drag locus markers. Compared with ‘Polalta’, the tobacco plant or germplasm provided by the invention not only has TSWD resistance, but also reduces or removes linkage drag.
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公开(公告)号：US20230160883A1
公开(公告)日：2023-05-25
申请号：US17947411
申请日：2022-09-19
发明人： Ning JIANG , Zhenyuan XIA , Xiaotong GAI , Zhonglong LIN , Canhua LU , Changjun HUANG , Junhong MA , Xiaohan MO , Wei WU , Chunming LIU , Qiuqiang HOU , Jian GUO
IPC分类号： G01N33/531 , C12N5/12 , G01N33/543 , G01N33/569
CPC分类号： G01N33/531 , C12N5/12 , G01N33/54388 , G01N33/56983 , C12N2510/02 , G01N2333/08
摘要： This invention provides a tomato zonate spot virus detection reagent. Optimized gene encoding N protein of TZSV is expressed and purified to obtain N protein of TZSV, and two specific hybridoma cell lines are obtained, from which specific anti-TZSV monoclonal antibody could be obtained, respectively. Test strip for detecting tomato zonate spot virus is prepared with the monoclonal antibody. It is proven by experiments that the test strip of this invention is specific for TZSV, and can realize rapid detection of TZSV in leaves and other samples, with high sensitivity and convenient operation.
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4.发明申请公开(公告)号：US20210120752A1
公开(公告)日：2021-04-29
申请号：US16648284
申请日：2017-09-20
发明人： Yong LIU , Changjun HUANG , Yongping LI , Haiqin YU
摘要： A TMV resistant tobacco plant containing a short N introgressed segment and a method for breeding the same. A homozygous tobacco plant containing an N introgressed segment is hybridized with a tobacco plant of genotype nn to obtain an F1 progeny tobacco plant of genotype Nn. The F1 progeny tobacco plant is hybridized with the tobacco plant of genotype nn, to obtain population materials for screening to obtain the short N introgressed segment. TMV is inoculated at a seedling stage, and Nn genotype plants showing necrotic lesion are obtained by screening the population materials. The Nn genotype plants are genotyped using a molecular marker TN5.51 primer pair and an N gene-specific molecular marker N1N2 at the right end of the N introgressed segment. A plant found to be negative when tested by the TN5.51 primer pair and to be positive when tested by the N1N2 molecular marker is a plant comprising the short N introgressed segment. The size of non-target genomic components deleted from plants containing the short N introgressed segment is estimated using TN5.34 and TN5.20 and TN4.99 primer pairs. The obtained short N introgressed segment is applicable to germplasma innovation and breeding of TMV resistant tobacco. The invention is helps to reduce linkage drag with the N gene.
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