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    LUCIFERIN-LUCIFERASE BASED MICRODEVICE FOR BIOSENSING
    3.
    发明申请
    LUCIFERIN-LUCIFERASE BASED MICRODEVICE FOR BIOSENSING 审中-公开
    LUCIFERIN-LUCIFERASE基于微生物鉴定的MICRODEVICE

    公开(公告)号：US20110091916A1

    公开(公告)日：2011-04-21

    申请号：US12988402

    申请日：2008-04-21

    申请人： Tao Pan ,  Zhenghong Sun ,  Wendy Wang ,  Xuanbin Liu

    发明人： Tao Pan ,  Zhenghong Sun ,  Wendy Wang ,  Xuanbin Liu

    IPC分类号： C12Q1/66 ,  C12M1/34

    CPC分类号： G01N21/763

    摘要： A method and apparatus for determining the concentration of one or more microbes in a sample is provided. This method involves filtering a sample through a filter inside a sample tube to retain the one or more microbes on the filter. The resulting filtrate, which contains or produces adenosine triphosphate, is passed through the sample tube and enters the reporter region. In the reporter region, the adenosine triphosphate in the filtrate comes in contact with a transparent porous matrix, which includes a luciferin-luciferase complex. The adenosine triphosphate interacts with the luciferin-luciferase complex to provide light response, which is measured by a detector. The light response is compared with a calibration curve to determine the total concentration of one or more microbes in a sample.

    摘要翻译： 提供了用于确定样品中一种或多种微生物浓度的方法和装置。 该方法包括通过样品管内的过滤器过滤样品以将一种或多种微生物保留在过滤器上。 将含有或产生三磷酸腺苷的所得滤液通过样品管并进入报道区。 在报道区域中，滤液中的三磷酸腺苷与透明的多孔基质接触，该基质包括荧光素 - 荧光素酶复合物。 三磷酸腺苷与荧光素 - 萤光素酶复合物相互作用以提供光响应，其由检测器测量。 将光响应与校准曲线进行比较，以确定样品中一种或多种微生物的总浓度。

    Apparatus and method of using porous material for antigen separation, identification, and quantification with electrophoresis techniques
    5.
    发明授权
    Apparatus and method of using porous material for antigen separation, identification, and quantification with electrophoresis techniques 有权
    使用多孔材料进行抗原分离，鉴定和电泳技术定量的装置和方法

    公开(公告)号：US08105844B2

    公开(公告)日：2012-01-31

    申请号：US12123714

    申请日：2008-05-20

    申请人： Zhen Hong Sun ,  Tao Pan ,  Wendy Wang ,  Xuanbin Liu

    发明人： Zhen Hong Sun ,  Tao Pan ,  Wendy Wang ,  Xuanbin Liu

    IPC分类号： G01N33/563

    CPC分类号： B01D57/02 ,  G01N33/582

    摘要： An apparatus includes a porous membrane for retaining antigens from a sample as the sample passes through the membrane. The apparatus also includes a first binding region within the membrane. The first binding region includes antibodies associated with a first antigen of interest. At least some of the antigens retained in the membrane are brought into contact with the first binding region by applying an electrophoresis field across the membrane. The porous membrane could also include an electrophoresis buffer. A presence of the first antigen of interest could be detected by exposing the first binding region to a chemiluminescent reagent, and a quantity of the first antigen of interest could be determined by performing a chemiluminescent assay on the binding region.

    摘要翻译： 一种装置包括当样品通过膜时用于保留来自样品的抗原的多孔膜。 该装置还包括膜内的第一结合区域。 第一结合区包括与感兴趣的第一抗原相关的抗体。 通过在膜上施加电泳场，保留在膜中的至少一些抗原与第一结合区接触。 多孔膜还可以包括电泳缓冲液。 可以通过将第一结合区域暴露于化学发光试剂来检测感兴趣的第一抗原的存在，并且可以通过在结合区域上进行化学发光测定来确定一定数量的目的第一抗原。

    APPARATUS AND METHOD OF USING POROUS MATERIAL FOR ANTIGEN SEPARATION, IDENTIFICATION, AND QUANTIFICATION WITH ELECTROPHORESIS TECHNIQUES
    6.
    发明申请
    APPARATUS AND METHOD OF USING POROUS MATERIAL FOR ANTIGEN SEPARATION, IDENTIFICATION, AND QUANTIFICATION WITH ELECTROPHORESIS TECHNIQUES 有权
    使用多孔材料进行抗电离技术的装置和方法进行抗分离，鉴定和定量

    公开(公告)号：US20090288953A1

    公开(公告)日：2009-11-26

    申请号：US12123714

    申请日：2008-05-20

    申请人： Zhen Hong Sun ,  Tao Pan ,  Wendy Wang ,  Xuanbin Liu

    发明人： Zhen Hong Sun ,  Tao Pan ,  Wendy Wang ,  Xuanbin Liu

    IPC分类号： B01D57/02

    CPC分类号： B01D57/02 ,  G01N33/582

    摘要： An apparatus includes a porous membrane for retaining antigens from a sample as the sample passes through the membrane. The apparatus also includes a first binding region within the membrane. The first binding region includes antibodies associated with a first antigen of interest. At least some of the antigens retained in the membrane are brought into contact with the first binding region by applying an electrophoresis field across the membrane. The porous membrane could also include an electrophoresis buffer. A presence of the first antigen of interest could be detected by exposing the first binding region to a chemiluminescent reagent, and a quantity of the first antigen of interest could be determined by performing a chemiluminescent assay on the binding region.

    摘要翻译： 一种装置包括当样品通过膜时用于保留来自样品的抗原的多孔膜。 该装置还包括膜内的第一结合区域。 第一结合区包括与感兴趣的第一抗原相关的抗体。 通过在膜上施加电泳场，保留在膜中的至少一些抗原与第一结合区接触。 多孔膜还可以包括电泳缓冲液。 可以通过将第一结合区域暴露于化学发光试剂来检测感兴趣的第一抗原的存在，并且可以通过在结合区域上进行化学发光测定来确定一定数量的目的第一抗原。

    MULTI-CHANNEL MICROARRAY APPARATUS AND METHODS
    7.
    发明申请
    MULTI-CHANNEL MICROARRAY APPARATUS AND METHODS 审中-公开
    多通道微型计算机和方法

    公开(公告)号：US20090088338A1

    公开(公告)日：2009-04-02

    申请号：US12325913

    申请日：2008-12-01

    申请人： Xuanbin Liu ,  Zhen Hong Sun ,  Wendy Wang ,  Tao Pan

    发明人： Xuanbin Liu ,  Zhen Hong Sun ,  Wendy Wang ,  Tao Pan

    IPC分类号： C40B30/04 ,  C40B60/12

    CPC分类号： G01N21/6452 ,  G01N21/648

    摘要： A multi-channel microarray reader has a light source carried by a first supporting stage, a second supporting stage having a plurality of reaction assembly receiving positions, each position to receive a reaction assembly, wherein each reaction assembly includes a reaction chamber and an optical substrate to support a microarray chip, and wherein the reaction chamber and the optical substrate encapsulate a buffer solution. The reader also includes an imaging sensor positioned to detect fluorescence emitted from a single microarray chip and a motion control module to position at least one of the first and second supporting stages to cause a selected microarray chip to receive energy emitted from the light source and to position the imaging sensor to receive fluorescence from that microarray chip.

    摘要翻译： 多通道微阵列读取器具有由第一支撑台承载的光源，具有多个反应组件接收位置的第二支撑台，每个位置接收反应组件，其中每个反应组件包括反应室和光学基板 以支持微阵列芯片，并且其中所述反应室和所述光学基板封装缓冲溶液。 读取器还包括成像传感器，其定位成检测从单个微阵列芯片发射的荧光和运动控制模块以定位第一和第二支持级中的至少一个，以使选定的微阵列芯片接收从光源发射的能量， 将成像传感器定位成从该微阵列芯片接收荧光。

    Programmable oligonucleotide micro array
    8.
    发明授权
    Programmable oligonucleotide micro array 有权
    可编程寡核苷酸微阵列

    公开(公告)号：US09005931B2

    公开(公告)日：2015-04-14

    申请号：US12810078

    申请日：2007-12-24

    申请人： Zhenhong Sun ,  Wendy Wang ,  Hang Liao

    发明人： Zhenhong Sun ,  Wendy Wang ,  Hang Liao

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  B01L7/00

    CPC分类号： C12Q1/6837 ,  B01L7/52 ,  C12Q1/686 ,  Y02A50/57 ,  C12Q2565/628 ,  C12Q2561/113 ,  C12Q2537/143 ,  C12Q2527/107

    摘要： A programmable probe design of DNA micro array and detection methodology is provided. DNA probes, which are complemented with the target DNA, are designed and classified into groups according to optimum hybridization temperature. The probes are arrayed by the group and immobilized on the substrate surface of the DNA micro array. The control system, imaging system and temperature control system are programmed to cooperate with each other during the detection process. This design increases the detection capabilities of the parallel-analysis system.

    摘要翻译： 提供了DNA微阵列和检测方法的可编程探针设计。 根据最适杂交温度，设计与靶DNA互补的DNA探针。 探针由组排列并固定在DNA微阵列的底物表面上。 控制系统，成像系统和温度控制系统被编程为在检测过程中相互配合。 该设计增加了并行分析系统的检测能力。