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公开(公告)号：US11565197B2
公开(公告)日：2023-01-31
申请号：US14898014
申请日：2014-06-12
摘要： A methodology scales supercritical fluid chromatography (SFC) and/or carbon dioxide based chromatography methods between different system and/or column configurations. The methodology includes measuring an average mobile phase density during a first separation utilizing C02 as a mobile phase component and substantially duplicating the average density profile for a second separation. Substantial duplication of the average mobile phase density (e.g., within about 10%, 5%, 2.5%, 1%, 0.5%, 0.1 %, 0.05%) results in chromatography for both system and/or column configurations having similar selectivity and retention factors. Average mobile phase density may be, either measured directly, calculated, or approximated using average pressure or density measurements. The average pressure profile may be used as a close approximation to duplicate average density profiles between separations.
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公开(公告)号：US11143634B2
公开(公告)日：2021-10-12
申请号：US16171826
申请日：2018-10-26
发明人： Abhijit Tarafder , Jason Hill , Joshua Shreve
摘要： Methodologies, systems, and computer-readable media are provided for controlling the mass flow rate within a CO2 based chromatography system. The pressure within a CO2 pump is measured and received at a computing system, and the computing system retrieves a target temperature value corresponding to the new pressure measurement within the CO2 pump. The computing system then generates a temperature control command that controls a CO2 pump heater or cooler in order to achieve the target temperature value at the CO2 pump. Thus, a target mass flow rate of CO2 from the CO2 pump is achieved by adjusting the temperature of the CO2 pump in response to changes in pressure.
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公开(公告)号：US11099159B2
公开(公告)日：2021-08-24
申请号：US16082368
申请日：2017-03-07
摘要： Reduced column loading of a sample onto a chromatographic column may be mitigated by excluding diffuse portions of the sample from introduction to the column. Systems and methods are provided for detecting and removing the diffuse portions from the feed solution delivered to the chromatography column. The systems and methods herein permit use of a single detector to detect and remove the diffuse portions and can accommodate a recovery/collection/recycling mechanism permitting reuse of removed sample from the diverted diffuse portions.
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公开(公告)号：US11085905B2
公开(公告)日：2021-08-10
申请号：US16439484
申请日：2019-06-12
摘要： Various embodiments are generally directed to techniques for monitoring chromatographic fluid flows, such as the flow to and/or from one or more reservoirs used in chromatographic operations, for instance. In many embodiments, the chromatographic operations may include one or more of High Performance Liquid Chromatography (HPLC), Ultra Performance Liquid Chromatography (UPLC), Ultra Performance Convergence Chromatography (UPC2), and the like. Several embodiments are particularly directed to a chromatographic fluid flow device (CFFD) for monitoring a change in density of a chromatographic fluid in a tube, such as by detecting the presence or absence of gas in the tube with an ultrasonic bubble detector. In various embodiments, the chromatographic fluid may include a solvent, a sample, or waste associated with a chromatographic operation.
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公开(公告)号：US20200150098A1
公开(公告)日：2020-05-14
申请号：US16678762
申请日：2019-11-08
摘要： The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a metering device for drawing up the liquid sample, a first multi-port valve in fluid communication with a first end of the metering device and the liquid sample, a second multi-port valve in fluid communication with a second end of the metering device and a chromatography column, and a pump in fluid communication with the second multi-port valve and a mobile phase. When the valves are in a first position the metering device draws up the liquid sample filling a portion of the metering device. When the valves are in a second position, a remaining portion of the metering device is filled with the mobile phase thereby mixing with and pressurizing the liquid sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.
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公开(公告)号：US20190265206A1
公开(公告)日：2019-08-29
申请号：US15769220
申请日：2016-10-19
发明人： Abhijit Tarafder
IPC分类号： G01N30/20
摘要： A significant reduction in extra-column band broadening can be achieved by decoupling the injection system from the main solvent flow line. Systems and methods for such decoupling can allow for the injection of larger volumes of sample without compromising separation yield, increase the column loading per batch, and increase the overall yield of separations. For example, a mixture of co-solvent and sample can be prepared separately from the main flow of mobile phase and co-solvent (e.g., a mixture of CO2 and methanol), loaded onto an injection loop, and then injected directly into the main flow of mobile phase and co-solvent before the chromatography column.
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公开(公告)号：US11340197B2
公开(公告)日：2022-05-24
申请号：US16678762
申请日：2019-11-08
摘要： The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a metering device for drawing up the liquid sample, a first multi-port valve in fluid communication with a first end of the metering device and the liquid sample, a second multi-port valve in fluid communication with a second end of the metering device and a chromatography column, and a pump in fluid communication with the second multi-port valve and a mobile phase. When the valves are in a first position the metering device draws up the liquid sample filling a portion of the metering device. When the valves are in a second position, a remaining portion of the metering device is filled with the mobile phase thereby mixing with and pressurizing the liquid sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.
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公开(公告)号：US20210055149A1
公开(公告)日：2021-02-25
申请号：US16996992
申请日：2020-08-19
摘要： Described is a method for determining a dwell volume of a liquid chromatography system and a liquid chromatography system that can determined the system dwell volume. The method includes mixing a flow of a first solvent with a flow of a second solvent to form a solvent mixture. The flows of the first and second solvents are decreased and increased, respectively, to generate a gradient composition. A system pressure of the liquid chromatography system is measured to determine a pressure trace defined as the measured system pressure as a function of time. The dwell volume of the system is determined from a time delay determined between the gradient composition at the mixing location and the pressure trace. The method can be performed with a liquid chromatography system having a chromatographic column or a flow restrictor used in place of the chromatographic column.
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公开(公告)号：US10578592B2
公开(公告)日：2020-03-03
申请号：US15310539
申请日：2015-05-12
发明人： Abhijit Tarafder
摘要： A method for estimating temperature variation due to pressure drop in a mobile phase passing through a column in a high efficiency chromatographic separation system is provided. The method includes constructing a temperature triangle using a pressure-temperature thermodynamic plot of the mobile phase having isenthalpic curves of the mobile phase. Applications of the method include development of chromatographic methods for minimizing band broadening due to temperature variation and methods to mitigate the extent of temperature variation due to the pressure drop.
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公开(公告)号：US20190277816A1
公开(公告)日：2019-09-12
申请号：US16292610
申请日：2019-03-05
摘要： A chromatographic device includes a primary channel having a cross-sectional area and characteristic length such that analyte travel within the primary channel is substantially convective. A plurality of secondary channels each having a cross-sectional area and characteristic length such that analyte flow into and out of a secondary channel is substantially diffusive, each of the plurality of secondary channels having an entrance in fluidic communication with the primary channel wherein the entrance intersects the primary channel.
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