公开(公告)号：US20230160901A1

公开(公告)日：2023-05-25

申请号：US17532871

申请日：2021-11-22

Applicant: Universiti Brunei Darussalam

Inventor： Minhaz Uddin AHMED ,  Juthi ADHIKARI ,  Mohammad RIZWAN

IPC: G01N33/577 ,  G01N21/66 ,  G01N33/58

CPC classification number: G01N33/577 ,  G01N21/66 ,  G01N33/583 ,  B82Y30/00

Abstract: The present application discloses an electrochemiluminescence (ECL) immunosensor. The ECL immunosensor includes an electrode modified by a nanocomposite comprising a mixture of carbon nanochips (CNCs); iron oxide (Fe3O4); and nafion (NAF). The electrode is a screen-printed electrode which further is a carbon screen-printed electrode (SPE). The carbon screen-printed electrode (SPE) is a mesoporous carbon screen-printed electrode (SPE). Ru(bpy)3Cl2.6H2O is a luminophore and TPrA is a coreactant of the luminophore.

公开(公告)号：US20230015484A1

公开(公告)日：2023-01-19

申请号：US17377389

申请日：2021-07-16

Applicant: Universiti Brunei Darussalam

Inventor： Nura Fazira NOOR AZAM ,  Minhaz Uddin AHMED ,  Noor Faizah MOHD-NAIM

IPC: G01N33/58 ,  C01G55/00 ,  H01F1/06 ,  G01N33/547 ,  G01N27/30 ,  G01N27/327 ,  G01N33/543 ,  G01N33/12 ,  G01N33/68

Abstract: The present application discloses an electrochemiluminescence immunosensor. The immunosensor includes an electrode functionalized by a nanocomposite film. The film further includes carbon nanohorns dispersed in Nafion® perfluorinated resin solution. The polymeric solution is further stabilized by magnetic nanoparticles. The immunosensor is a Point of care (POC)-based. The immunosensor is configured to work in the range from 100 ng/mL to 1 fg/mL, and has tendency to detect even traces of the tropomyosin. The immunosensor is capable to detect traces even less than 1 fg/mL, hence having high specificity for Tro-Ag detection in food products with distinguished repeatability.

公开(公告)号：US20180037944A1

公开(公告)日：2018-02-08

申请号：US15672233

申请日：2017-08-08

Applicant: Universiti Brunei Darussalam

Inventor： Sharmili ROY ,  Minhaz Uddin AHMED

IPC: C12Q1/68

CPC classification number: C12Q1/6846 ,  C12Q1/6816 ,  C12Q1/6881 ,  C12Q1/689 ,  C12Q2563/103 ,  C12Q2565/607

Abstract: Present invention disclosed a novel method for detecting and quantifying target DNA from the biological sample. It provides a method of amplification of DNA sequences with loop-mediated isothermal amplification (LAMP) and its easy and accurate detection and quantification by electrochemiluminescence (ECL) technique. The target LAMP DNA is bound electrostatically with [Ru(bpy)3]+2 on the carbon electrode surface, and an ECL reaction is triggered by tripropylamine (TPrA) to yield luminescence. This is a highly sensitive strategy for the detection of sequence-specific DNA from different biological samples at picogram levels. The target DNA of Sus scrofa (pork) meat was detected as low as 1 pg/μL (3.43×10−1 copies/μL) and for Bacillus subtilis DNA samples the detection limit of 10 pg/μL (2.2×103 copies/μL) was achieved.

公开(公告)号：US20200217801A1

公开(公告)日：2020-07-09

申请号：US16819961

申请日：2020-03-16

Applicant: UNIVERSITI BRUNEI DARUSSALAM

Inventor： Minhaz Uddin AHMED ,  Mohammad RIZWAN

IPC: G01N21/76 ,  G01N33/68 ,  C09K11/07

Abstract: The present invention discloses an electrochemical immunosensor (ECL). The ECL is configured for detecting Haptoglobin in biological samples. The immunosensor includes nanocomposite of gold nanoparticles, single-walled carbon nanotubes, quantum dots, and chitosan. The immunosensor can also be used for the ECL based detection of other biomarkers and biomolecules, such as Immunoglobulin A and dopamine.

公开(公告)号：US20180037959A1

公开(公告)日：2018-02-08

申请号：US15672194

申请日：2017-08-08

Applicant: Universiti Brunei Darussalam

Inventor： Hamadah LUBIS ,  Minhaz Uddin AHMED

IPC: C12Q1/68 ,  G01N33/12

CPC classification number: C12Q1/6888 ,  C12Q1/6818 ,  C12Q2600/158 ,  G01N33/12

Abstract: A rapid, sensitive and cost-effective process of detection of porcine contamination in a food product sample is provided herein. The present invention further provides a real-time polymerase chain reaction (PCR) based process which uses highly specific oligonucleotides primers and ZEN™ probe, and a kit that contains the primers and/or probe useful for rapid detection of porcine DNA in a food sample. The oligonucleotide primers disclosed in the present invention provide amplification of the target gene porcine (Sus scrofa) mitochondrial 12S ribosomal RNA gene in highly sensitive and specific manner showing no cross amplification.

公开(公告)号：US20240192220A1

公开(公告)日：2024-06-13

申请号：US18583498

申请日：2024-02-21

Applicant: Universiti Brunei Darussalam

Inventor： Nura Fazira NOOR AZAM ,  Minhaz Uddin AHMED ,  Noor Faizah MOHD-NAIM

IPC: G01N33/58 ,  B82Y5/00 ,  B82Y25/00 ,  B82Y30/00 ,  B82Y35/00 ,  B82Y40/00 ,  C01G55/00 ,  G01N27/30 ,  G01N27/327 ,  G01N33/12 ,  G01N33/543 ,  G01N33/547 ,  G01N33/68 ,  H01F1/06

CPC classification number: G01N33/587 ,  C01G55/004 ,  G01N27/308 ,  G01N27/3278 ,  G01N33/12 ,  G01N33/54366 ,  G01N33/547 ,  G01N33/582 ,  G01N33/68 ,  H01F1/068 ,  B82Y5/00 ,  B82Y25/00 ,  B82Y30/00 ,  B82Y35/00 ,  B82Y40/00 ,  C01P2004/64 ,  C01P2006/42

Abstract: The present application discloses an electrochemiluminescence immunosensor. The immunosensor includes an electrode functionalized by a nanocomposite film. The film further includes carbon nanohorns dispersed in Nafion® perfluorinated resin solution. The polymeric solution is further stabilized by magnetic nanoparticles. The immunosensor is a Point of care (POC)-based. The immunosensor is configured to work in the range from 100 ng/mL to 1 fg/mL, and has tendency to detect even traces of the tropomyosin. The immunosensor is capable to detect traces even less than 1 fg/mL, hence having high specificity for Tro-Ag detection in food products with distinguished repeatability.

公开(公告)号：US20240192219A1

公开(公告)日：2024-06-13

申请号：US18583424

申请日：2024-02-21

Applicant: Universiti Brunei Darussalam

Inventor： Nura Fazira NOOR AZAM ,  Minhaz Uddin AHMED ,  Noor Faizah MOHD-NAIM

IPC: G01N33/58 ,  B82Y5/00 ,  B82Y25/00 ,  B82Y30/00 ,  B82Y35/00 ,  B82Y40/00 ,  C01G55/00 ,  G01N27/30 ,  G01N27/327 ,  G01N33/12 ,  G01N33/543 ,  G01N33/547 ,  G01N33/68 ,  H01F1/06

CPC classification number: G01N33/587 ,  C01G55/004 ,  G01N27/308 ,  G01N27/3278 ,  G01N33/12 ,  G01N33/54366 ,  G01N33/547 ,  G01N33/582 ,  G01N33/68 ,  H01F1/068 ,  B82Y5/00 ,  B82Y25/00 ,  B82Y30/00 ,  B82Y35/00 ,  B82Y40/00 ,  C01P2004/64 ,  C01P2006/42

Abstract: The present application discloses an electrochemiluminescence immunosensor. The immunosensor includes an electrode functionalized by a nanocomposite film. The film further includes carbon nanohorns dispersed in Nafion® perfluorinated resin solution. The polymeric solution is further stabilized by magnetic nanoparticles. The immunosensor is a Point of care (POC)-based. The immunosensor is configured to work in the range from 100 ng/mL to 1 fg/mL, and has tendency to detect even traces of the tropomyosin. The immunosensor is capable to detect traces even less than 1 fg/mL, hence having high specificity for Tro-Ag detection in food products with distinguished repeatability.