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1.发明授权Scanner-type fluorescence detection apparatus using small sized excitation light source 失效使用小型激发光源的扫描仪型荧光检测仪公开(公告)号：US06515743B1
公开(公告)日：2003-02-04
申请号：US09667722
申请日：2000-09-22
IPC分类号： G01N2164
CPC分类号： G01N21/6452 , G01N2021/6484 , G01N2201/0423
摘要： A fluorescence detection apparatus comprising: a sample holder for fixing and holding sample vessels on a circular arc; a partition plate being joined to drive means for rotation on the center of the circular arc; an excitation light source; excitation light optical means; and fluorescence optical means containing a light guide being fixed on the partition plate for rotation integrally and a photosensor. The photosensor is mechanically discontinued from the drive means and fixedly placed. The fluorescence signal emission end of the light guide is placed facing the photosensor on the rotation center axis and the partition plate and the parts fixed thereto are rotated integrally, whereby fluorescence detection of the samples arranged on the circular arc is repeated in order.
摘要翻译： 一种荧光检测装置，包括：用于将样品容器固定并保持在圆弧上的样品保持器; 一个隔板连接到驱动装置上，用于在圆弧的中心旋转; 激发光源; 激发光光学装置; 以及含有固定在隔板上的导光体的荧光光学装置一体地旋转，光传感器。 光传感器从驱动装置机械地停止并固定放置。 将导光体的荧光信号发射端面朝向旋转中心轴的光电传感器放置，分隔板和固定于其上的部件一体旋转，由此依次重复排列在圆弧上的样品的荧光检测。
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2.发明授权Fluorescence detection method capable of making measurement under external light 失效能够在外部光线下进行测量的荧光检测方法公开(公告)号：US06664113B2
公开(公告)日：2003-12-16
申请号：US10126629
申请日：2002-04-22
IPC分类号： G01N2164
CPC分类号： G01N21/6452 , G01N2201/0642 , Y10T436/25 , Y10T436/2575
摘要： A method for detecting fluorescence from a liquid sample housed in a transparent or translucent sample container includes providing a sample container in a sample holder, which is opaque except for a sample container introduction opening and incoming and outgoing openings for excitation light; layering a liquid sample and a shielding liquid unmixable therewith to prevent external light from entering through the sample container introduction opening; introducing excitation light from such a direction that the excitation light can irradiate the liquid sample before irradiating the shielding liquid; and detecting fluorescence, which emits in a direction of avoiding absorption by the shielding liquid.
摘要翻译： 用于检测容纳在透明或半透明样品容器中的液体样品的荧光的方法包括：将样品容器放置在样品保持器中，样品容器除了样品容器引入开口和用于激发光的入射和出射开口外是不透明的; 层叠液体样品和与其不可混合的屏蔽液体，以防止外部光线通过样品容器引入口; 在照射所述屏蔽液体之前，从所述激发光可以照射所述液体样品的方向引入激发光; 并检测荧光，其沿避开被屏蔽液体吸收的方向发射。
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3.发明授权公开(公告)号：US06396581B1
公开(公告)日：2002-05-28
申请号：US09492302
申请日：2000-01-27
IPC分类号： G01N2164
CPC分类号： G01N21/645 , G01N21/253 , G01N35/025 , G01N2201/0231 , G01N2201/0423 , G01N2201/043 , G01N2201/0806 , G01N2201/0853
摘要： A fluorescence detection apparatus is provided which comprises a sample holder for holding stationarily sample vessels deployed along a circle line or concentric circle lines having different radiuses, a partition plate connected to a driving means to be rotatable around the center of the circle line or concentric circle lines, an optical means for excitation light and an optical means for fluorescence light fixed respectively to the partition plate to be rotatable in integration therewith, a first light guide constituted of numerous optical fibers, a photosensor, and a light source for generating the excitation light, wherein the partition plate, the optical means for excitation light, and the optical means for fluorescence are integrally rotated, and thereby the fluorescence of the sample arranged along the circle line is successively detected and the detected fluorescence is transmitted to the photosensor. This fluorescence detection apparatus is useful in real-time monitoring of fluorescence signals, and satisfies the requirements of precise temperature control, quick treatment of many samples, high sensitivity, high reliability, low cost, and small size of the apparatus.
摘要翻译： 提供了一种荧光检测装置，其包括用于保持沿着圆线展开的固定样品容器的样本保持器或具有不同半径的同心圆线，连接到驱动装置的分隔板，以可围绕圆线或同心圆的中心旋转 线路，用于激发光的光学装置和用于荧光的光学装置分别固定在分隔板上以使其一体旋转;由多个光纤构成的第一导光体，光电传感器和用于产生激发光的光源 其中分隔板，用于激发光的光学装置和用于荧光的光学装置一体地旋转，从而连续检测沿着圆线排列的样品的荧光，并将检测到的荧光传输到光电传感器。 该荧光检测装置可用于实时监测荧光信号，满足精密温度控制，快速处理多种样品，高灵敏度，高可靠性，低成本，体积小的要求。
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公开(公告)号：US06359284B1
公开(公告)日：2002-03-19
申请号：US09391161
申请日：1999-09-08
IPC分类号： G01N3504
CPC分类号： G01N21/645 , G01N2021/6482 , G01N2021/6484 , G01N2201/0423
摘要： An apparatus including a sample holder which fixedly holds sample vessels arranged on a same arc, a partition plate, a light guide which is configured by optical fibers and which transmits fluorescent signals emitted from respective test samples, to an optical sensor, the single optical sensor, and a light source which generates excitation light. Fluorescent signal emission ends of the light guide are opposed to the optical sensor, and fluorescent signal incidence ends of the light guide are respectively opposed to the sample vessels via the partition plate therebetween. The partition plate includes an excitation light optical unit for selectively guiding the excitation light from the light source to only one of the sample vessels arranged on the arc, and a fluorescence optical unit for guiding only the fluorescent signal emitted from a selected one of the sample vessels to the light guide. The partition plate is coupled together with the excitation light optical unit and the fluorescence optical unit to a driving unit, to be rotatable about a center of the arc on which the sample vessels are arranged. Fluorescence is detected while the excitation light is guided sequentially to the sample vessels arranged on the arc, by rotation of the partition plate.
摘要翻译： 一种装置，包括固定地保持设置在同一弧上的样本容器的样本保持器，隔板，由光纤构成并将从各测试样本发射的荧光信号传输到光学传感器的光导，单个光学传感器 ，以及产生激发光的光源。 光导体的荧光信号发射端与光学传感器相对，并且光导的荧光信号入射端分别通过其间的隔板与样品容器相对。 分隔板包括用于选择性地将来自光源的激发光引导到仅布置在电弧上的一个样品容器的激发光光学单元，以及用于仅引导从所选样品中发射的荧光信号的荧光光学单元 船只到光导。 分隔板与激发光光学单元和荧光光学单元耦合到驱动单元，以围绕放置样品容器的弧的中心旋转。 通过分隔板的旋转，激发光被顺序地引导到布置在电弧上的样品容器中，检测荧光。
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5.发明授权Oligonucleotides and method for characterizing and detecting Genogroup II type small round structured virus 失效用于表征和检测Genogroup II型小圆结构病毒的寡核苷酸和方法公开(公告)号：US07339041B2
公开(公告)日：2008-03-04
申请号：US10441126
申请日：2003-05-20
IPC分类号： C07H21/04
CPC分类号： C12Q1/701
摘要： Nucleic acid sequences, oligonucleotides and a method for detection of SRSV, in particular, a virus which belongs to Genotype II (GII), in clinical examinations, public health examinations, food evaluations and food poisoning examinations are provided.
摘要翻译： 提供了核酸序列，寡核苷酸和用于检测SRSV的方法，特别是属于基因型II（GII）的病毒，用于临床检查，公众健康检查，食物评估和食物中毒检查。
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公开(公告)号：US20060134686A1
公开(公告)日：2006-06-22
申请号：US11350920
申请日：2006-02-10
CPC分类号： C12N9/1276
摘要： An avian myeloblastosis virus reverse transcriptase composition which comprises a heterodimer consisting of an α subunit having a molecular weight of 68000 and a β subunit having a molecular weight of 92000 in an amount of at least 80%.
摘要翻译： 一种禽成髓细胞病毒病毒逆转录酶组合物，其包含由分子量为68000的α亚单位组成的异源二聚体和分子量为92000的β亚基，其量至少为80％。
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公开(公告)号：US20050208547A1
公开(公告)日：2005-09-22
申请号：US11047722
申请日：2005-02-02
IPC分类号： C07H21/04 , C12Q1/68 , C12Q1/6865 , C12Q1/689 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/6865 , Y02A50/451
摘要： The present invention provides oligonucleotides for detecting Salmonella toxin gene invA mRNA and stn mRNA which oligonucleotides specifically bind to invA mRNA or stn mRNA at a relatively low temperature (for example, 41° C.) and at a constant temperature, and a process of amplifying Salmonella toxin gene invA mRNA or stn mRNA and a method of detecting the same using the oligonucleotides.
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公开(公告)号：US06211354B1
公开(公告)日：2001-04-03
申请号：US09305223
申请日：1999-05-05
申请人： Ryuichi Horie , Takahiko Ishiguro
发明人： Ryuichi Horie , Takahiko Ishiguro
IPC分类号： C07H2104
CPC分类号： C07H21/00
摘要： A DNA probe of the following structural formula (1) (wherein P* is an optically active phosphorus atom, each of R1 and R2 is a DNA oligomer having an arbitrary sequence, and R3 is a fluorescent intercalative dye attached via an appropriate linker) which has an optically active configuration about P*.
摘要翻译： 以下结构式（1）的DNA探针（其中P *为光学活性磷原子，R1和R2各自为具有任意序列的DNA低聚物，R3为通过适当的连接体连接的荧光插入染料） 具有关于P *的光学配置。
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9.发明授权Oligonucleotides and method for detection of meca gene of methicillin-resistant Staphylococcus aureus 有权寡核苷酸和检测耐甲氧西林金黄色葡萄球菌麦卡基因的方法公开(公告)号：US07297517B2
公开(公告)日：2007-11-20
申请号：US09865579
申请日：2001-05-29
申请人： Toshiki Taya , Takahiko Ishiguro , Juichi Saito
发明人： Toshiki Taya , Takahiko Ishiguro , Juichi Saito
IPC分类号： C12P19/34
CPC分类号： C12Q1/689 , C12Q1/6816 , C12Q1/6865 , C12Q2600/158 , C12Q2565/107 , C12Q2563/173
摘要： An oligonucleotide for cleavage, detection or amplification of the mecA gene, a gene element of methicillin-resistant Staphylococcus aureus (MRSA), or RNA derived from said gene is provided. Further, a method for detecting the mecA gene is provided.
摘要翻译： 提供了用于mecA基因的切割，检测或扩增的寡核苷酸，耐甲氧西林金黄色葡萄球菌（MRSA）的基因元件或源自所述基因的RNA。 此外，提供了检测mecA基因的方法。
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公开(公告)号：US20060099637A1
公开(公告)日：2006-05-11
申请号：US11313849
申请日：2005-12-22
CPC分类号： C12Q1/6865 , C12Q1/689 , C12Q2527/101
摘要： An oligonucleotide capable of binding to the intramolecular structure-free region of Verotoxin type 1 RNA or Verotoxin type 2 RNA at relatively low and constant temperature, and which can be used in a constant temperature nucleic acid amplification method, is provided. Also, a simple, speedy and highly sensitive method for detecting Verotoxin type 1 RNA or Verotoxin type 2 RNA is provided.
摘要翻译： 提供了能够在相对低且恒定的温度下结合到Verotoxin 1型Verotoxin或2型RNA的分子内无结构域的寡核苷酸，并且可以用于恒温核酸扩增方法。 此外，还提供了一种用于检测Verotoxin 1型RNA或Verotoxin 2型RNA的简单，快速和高度灵敏的方法。
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