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    Methods and Compositions for Spacial and Temporal Measurement of Catalytic Activity
    1.
    发明申请
    Methods and Compositions for Spacial and Temporal Measurement of Catalytic Activity 有权

    公开(公告)号：US20210096129A1

    公开(公告)日：2021-04-01

    申请号：US16603722

    申请日：2018-04-12

    Applicant: The University of Chicago

    Inventor： Yamuna Krishnan ,  Krishna Dan ,  Aneesh T. Veetil ,  Kasturi Chakraborty

    IPC: G01N33/573 ,  G01N21/64 ,  C07H21/04

    Abstract: Described herein are nucleic acid molecules and complexes useful for spatiotemporally mapping intra-endosmal thiol disulphide exchange. Aspects of the disclosure relate to a composition comprising a first nucleic acid conjugated to a normalization moiety; and a second nucleic acid conjugated to a catalytic substrate; wherein reaction of the substrate with a catalyst produces a detectable product; and wherein the first and second nucleic acids are complementary or substantially complementary. Further aspects relate to a composition comprising: a first nucleic acid conjugated to a normalization moiety and to a catalytic substrate; and a second nucleic acid; wherein reaction of the substrate with a catalyst produces a detectable product; and wherein the first and second nucleic acids are complementary or substantially complementary.

    Methods and compositions for spacial and temporal measurement of catalytic activity
    2.
    发明授权
    Methods and compositions for spacial and temporal measurement of catalytic activity 有权

    公开(公告)号：US12216121B2

    公开(公告)日：2025-02-04

    申请号：US16603722

    申请日：2018-04-12

    Applicant: The University of Chicago

    Inventor： Yamuna Krishnan ,  Krishna Dan ,  Aneesh T. Veetil ,  Kasturi Chakraborty

    IPC: G01N33/573 ,  C07H21/04 ,  G01N21/64

    Abstract: Described herein are nucleic acid molecules and complexes useful for spatiotemporally mapping intra-endosmal thiol disulphide exchange. Aspects of the disclosure relate to a composition comprising a first nucleic acid conjugated to a normalization moiety; and a second nucleic acid conjugated to a catalytic substrate; wherein reaction of the substrate with a catalyst produces a detectable product; and wherein the first and second nucleic acids are complementary or substantially complementary. Further aspects relate to a composition comprising: a first nucleic acid conjugated to a normalization moiety and to a catalytic substrate; and a second nucleic acid; wherein reaction of the substrate with a catalyst produces a detectable product; and wherein the first and second nucleic acids are complementary or substantially complementary.

    Methods and Compositions for Determining pH
    7.
    发明申请
    Methods and Compositions for Determining pH 有权

    公开(公告)号：US20210403987A1

    公开(公告)日：2021-12-30

    申请号：US17466474

    申请日：2021-09-03

    Applicant: The University of Chicago ,  National Centre for Biological Sciences

    Inventor： Yamuna Krishnan ,  Saheli Halder

    IPC: C12Q1/6809 ,  C12N15/113 ,  C12N15/11 ,  G01N33/84 ,  C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/6876

    Abstract: Described herein are nucleic acid molecules and complexes useful as i-switch pH reporters that have increased sensitivities as a pH reporter and have alternate pH reporting capacity ranges. Aspects of the disclosure relate to a method for determining pH comprising providing a nucleic acid complex comprising: a first single-stranded nucleic acid molecule comprising the sequence CnXCnYCnZCn (SEQ ID NO. 6) wherein C is cytosine; X, Y and Z are each one or more of adenine, thymine, guanine, or combinations thereof; and n is greater than or equal to 2; and wherein at least 2 cytosine residues of the first single-stranded nucleic acid molecule are modified; and a second single-stranded nucleic acid molecule that is partially or fully complementary to the first single-stranded molecule, wherein a first label is conjugated to the first single-stranded nucleic acid molecule or the second single-stranded nucleic acid molecule; and wherein the first label is capable of producing a signal, wherein the intensity of the signal varies as a function of the conformation of the nucleic acid complex; and measuring the intensity of the signal and determining the pH from the measured signal.

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