公开(公告)号：US20220196685A1

公开(公告)日：2022-06-23

申请号：US17603205

申请日：2020-04-29

申请人： The Regents of the University of California

发明人： Christopher J. Chang ,  Yik Sham Clive Chung ,  Sumin Lee

IPC分类号： G01N33/84 ,  G01N33/542

摘要： The present disclosure provides Forster resonance energy transfer (FRET)-based probes for detecting copper, e.g., for detecting Cu(I) in live cells. The present disclosure provides methods for detecting copper, e.g., for detecting Cu(I) in live cells, using a FRET-based probe of the present disclosure.

公开(公告)号：US20220001357A1

公开(公告)日：2022-01-06

申请号：US17478874

申请日：2021-09-17

申请人： The Regents of the University of California

发明人： Sumin Lee ,  Adam Uliana ,  Jeffrey R. Long ,  Christopher J. Chang

IPC分类号： B01J20/26 ,  B01J20/30 ,  G01N31/22 ,  C02F1/28

摘要： Ether-thioether functionalized porous aromatic framework (PAF) polymers provide high selectivity for iron(II) and iron(III) adsorption in aqueous samples.

公开(公告)号：US20210238212A1

公开(公告)日：2021-08-05

申请号：US17164281

申请日：2021-02-01

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Jeffrey S. Derrick ,  Matthias Loipersberger ,  Jeffrey R. Long ,  Martin Head-Gordon ,  Christopher J. Chang

IPC分类号： C07F15/02 ,  B01J31/18 ,  C25B3/26

摘要： The electrochemical carbon dioxide reduction reaction (CO2RR) provides opportunities to synthesize value-added products from this greenhouse gas in a sustainable manner. Efficient catalysts for this reaction are provided that selectively drive CO2 reduction over the thermodynamic and kinetically competitive hydrogen evolution reaction (HER) in organic or aqueous electrolytes. The catalysts are metal-polypyridyl coordination complexes of a redox non-innocent terpyridine-based pentapyridine ligand and a first-row transition metal. The metal-ligand cooperativity in [Fe(tpyPY2Me)]2+ drives the electrochemical reduction of CO2 to CO at low overpotentials with high selectivity for CO2RR (>90%).

公开(公告)号：US20210230215A1

公开(公告)日：2021-07-29

申请号：US17207609

申请日：2021-03-20

申请人： The Regents of the University of California

发明人： Christopher J. Chang ,  Shang Jia

IPC分类号： C07K1/13 ,  C07K1/113

摘要： Proteins are labeled by contacting the protein with a thiophosphorodichloridate reagent under conditions to effect chemoselective histidine conjugation.

公开(公告)号：US20190257838A1

公开(公告)日：2019-08-22

申请号：US16402113

申请日：2019-05-02

申请人： The Regents of the University of California

发明人： ShiXian Lin ,  XiaoYu Yang ,  F. Dean Toste ,  Christopher J. Chang

IPC分类号： G01N33/68 ,  G16C20/10 ,  G16C20/70 ,  C12Q1/02 ,  C12Q1/26

摘要： Chemoselective conjugation is achieved through redox reactivity by reacting an N-transfer oxidant with a thioether substrate in a redox reaction in an aqueous environment to form a conjugation product. In embodiments, Redox-Activated Chemical Tagging (ReACT) strategies for methionine-based protein functionalization. Oxaziridine (Ox) compounds serve as oxidant-mediated reagents for direct functionalization by converting methionine to the corresponding sulfimide conjugation product.

公开(公告)号：US09329191B2

公开(公告)日：2016-05-03

申请号：US14010327

申请日：2013-08-26

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Christopher J. Chang ,  Brian William Michel

IPC分类号： G01N33/84 ,  C07F15/00 ,  G01N33/58

CPC分类号： G01N33/84 ,  C07F15/006 ,  G01N33/581

摘要： Carbon monoxide (CO) is a member of the gasotransmitter family that includes NO and H2S and is implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. We now report a new type of turn-on fluorescent probe for selective CO detection by exploiting palladium-mediated carbonylation reactivity. The compounds of the invention are capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.

摘要翻译： 一氧化碳（CO）是包含NO和H2S的气体传递器家族的成员，并且涉及各种病理和生理条件。 尽管对组织和整个动物的CO的外源性治疗添加已被很好地研究，但在细胞水平上CO的实时空间和时间跟踪仍然是一个公开的挑战。 我们现在报告一种新型的开启荧光探针，通过利用钯介导的羰基化反应性进行选择性CO检测。 本发明的化合物能够在水性缓冲液和活细胞中在一定范围的生物相关的反应性小分子上以高选择性检测CO，为生物系统中询问其化学作用提供了潜在的有效方法。

公开(公告)号：US12077557B2

公开(公告)日：2024-09-03

申请号：US16981511

申请日：2019-03-20

申请人： The Regents of the University of California

发明人： Christopher J. Chang ,  Yik Sham Clive Chung

IPC分类号： C07H19/16 ,  C12Q1/26 ,  G01N33/58

CPC分类号： C07H19/16 ,  C12Q1/26 ,  G01N33/582

摘要： The present disclosure provides puromycin-based probes, as well as compositions and kits comprising the probes. The present disclosure provides methods of detecting an analyte, and imaging methods, using the probes.

公开(公告)号：US10730892B2

公开(公告)日：2020-08-04

申请号：US15747125

申请日：2016-08-18

申请人： The Regents of the University of California

发明人： Christopher J. Chang ,  Thomas Francis Brewer ,  Jefferson Chan

IPC分类号： C07F7/08 ,  C09K11/06 ,  G01N1/30 ,  C12Q1/26 ,  G01N21/76 ,  G01N15/14

摘要： Probes for formaldehyde (FA) including a homoallylamine trigger group attached to a detectable moiety are provided. Aspects of the probes include luminogenic or fluorogenic probes, such as a probe including a quencher in energy-receiving proximity to a fluorophore. Also provided are methods of using the probes for sensitive and bio orthogonal detection of FA in a sample. Aspects of the methods include selectively reacting the probe with the formaldehyde in the sample to a release (e.g., via a 2-aza-Cope rearrangement) a reporter group comprising a detectable moiety. Aspects of the methods detecting formaldehyde in a cell, tissue, organ or fluid in a subject. Also provided are compositions and kits including the subject probes that find use in practicing various embodiments of the subject methods.

公开(公告)号：US10605799B2

公开(公告)日：2020-03-31

申请号：US15593117

申请日：2017-05-11

申请人： The Regents of the University of California

发明人： Christopher J. Chang ,  Jeffrey R. Long ,  Sumin Lee ,  Gokhan Barin

IPC分类号： G01N33/00 ,  G01N33/487 ,  C22B3/26 ,  G01N33/84 ,  G01N33/53 ,  B01J20/30 ,  G01N31/22

摘要： A method of selective detection of a concentration of a metal ion species in a subject is provided in which a biofluid sample is obtained from the subject. The biofluid sample is exposed to a functionalized porous aromatic polymer. The polymer selectively captures and concentrates the metal ion species from the biofluid. Subsequently, the biofluid is washed from the polymer. The polymer is then exposed to a solution comprising a colorimetric indicator that extracts the metal ion species from the washed polymer thereby changing a color of the solution as a function of an amount of the metal ion species in the polymer. The concentration of the metal ion species in the subject is then spectroscopically determined from the color of the solution.

公开(公告)号：US08865914B2

公开(公告)日：2014-10-21

申请号：US13830767

申请日：2013-03-14

申请人： The Regents of the University of California

发明人： Christopher J. Chang ,  Genevieve C. Van De Bittner ,  Tasuku Hirayama ,  Jefferson Chan

IPC分类号： C07D311/86 ,  C07D307/94 ,  C07D403/08 ,  C07F7/10 ,  C07F5/04 ,  C07D311/82 ,  A61K49/00 ,  C07D209/12 ,  C07D207/323

CPC分类号： C07D307/94 ,  A61K49/0041 ,  A61K49/0052 ,  C07D207/323 ,  C07D209/12 ,  C07D311/82 ,  C07D311/86 ,  C07D403/08 ,  C07F5/022 ,  C07F5/04 ,  C07F7/081 ,  C07F7/10 ,  C07F7/1804 ,  G01N33/582 ,  G01N33/84

摘要： The invention provides fluorescent sensors for the selective detection of a metal such as copper. The sensors may be considered to be derivatives of cyanine, fluorescein, rhodamine, rhodol, Tokyo green, or BODIPY. The sensors find particular use in detecting copper in cells and living animals.

摘要翻译： 本发明提供用于选择性检测诸如铜的金属的荧光传感器。 传感器可以被认为是花青，荧光素，罗丹明，rhodol，Tokyo green或BODIPY的衍生物。 传感器特别用于检测细胞和活体动物中的铜。