公开(公告)号：US20130090470A1

公开(公告)日：2013-04-11

申请号：US13614948

申请日：2012-09-13

申请人： Schuyler CORRY ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  Lawrence Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

发明人： Schuyler CORRY ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  Lawrence Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

IPC分类号： C07D503/00 ,  C07F5/02 ,  C07D501/58

CPC分类号： C07F5/022 ,  C07D499/00 ,  C07D501/00 ,  C07D501/58 ,  C07D501/60 ,  C07D503/00 ,  C07D519/00 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N33/581 ,  G01N2333/986

摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

摘要翻译： 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，还公开了分析样品中存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。 公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。

公开(公告)号：US08865891B2

公开(公告)日：2014-10-21

申请号：US13614948

申请日：2012-09-13

申请人： Schuyler Corry ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  Lawrence Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

发明人： Schuyler Corry ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  Lawrence Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

IPC分类号： C07D501/36 ,  C07D501/58 ,  C07D501/00 ,  C07F5/02 ,  C07D503/00 ,  C07D499/00 ,  C12Q1/34

CPC分类号： C07F5/022 ,  C07D499/00 ,  C07D501/00 ,  C07D501/58 ,  C07D501/60 ,  C07D503/00 ,  C07D519/00 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N33/581 ,  G01N2333/986

摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactamase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactamase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactamase or instructions for using the components in an assay.

摘要翻译： 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品中β-内酰胺酶的存在的方法，例如，作为包括编码β-内酰胺酶的序列的核酸序列的表达的指示剂。 公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-内酰胺酶或在测定中使用组分的说明书。

公开(公告)号：US08318450B2

公开(公告)日：2012-11-27

申请号：US13166627

申请日：2011-06-22

申请人： Schuyler Corry ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  Lawrence Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

发明人： Schuyler Corry ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  Lawrence Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

IPC分类号： C12Q1/34

CPC分类号： C07F5/022 ,  C07D499/00 ,  C07D501/00 ,  C07D501/58 ,  C07D501/60 ,  C07D503/00 ,  C07D519/00 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N33/581 ,  G01N2333/986

摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

摘要翻译： 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。 公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。

公开(公告)号：US20050227309A1

公开(公告)日：2005-10-13

申请号：US11040924

申请日：2005-01-21

申请人： Schuyler Corry ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  I. Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

发明人： Schuyler Corry ,  William Downey ,  Brian Filanoski ,  Kyle Gee ,  I. Greenfield ,  James Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

IPC分类号： C07D487/08 ,  C07D499/00 ,  C07D501/00 ,  C07D501/14 ,  C07D503/00 ,  C07D519/00 ,  C07F5/02 ,  C09B1/00 ,  C12Q1/18 ,  C12Q1/34

CPC分类号： C07F5/022 ,  C07D499/00 ,  C07D501/00 ,  C07D501/58 ,  C07D501/60 ,  C07D503/00 ,  C07D519/00 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N33/581 ,  G01N2333/986

摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

摘要翻译： 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品中存在β-内酰胺酶的方法，例如作为包括编码β-内酰胺酶的序列的核酸序列的表达的指示剂。 公开了包括公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-内酰胺酶或在测定中使用组分的说明书。

公开(公告)号：US20090047692A1

公开(公告)日：2009-02-19

申请号：US12099085

申请日：2008-04-07

申请人： Schuyler Boon CORRY ,  William Louis Downey ,  Brian Filanoski ,  Kyle Richard Gee ,  I. Lawrence Greenfield ,  James David Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

发明人： Schuyler Boon CORRY ,  William Louis Downey ,  Brian Filanoski ,  Kyle Richard Gee ,  I. Lawrence Greenfield ,  James David Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

IPC分类号： G01N33/53 ,  C07D501/60 ,  C07D498/04 ,  C12Q1/34

CPC分类号： C07F5/022 ,  C07D499/00 ,  C07D501/00 ,  C07D501/58 ,  C07D501/60 ,  C07D503/00 ,  C07D519/00 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N33/581 ,  G01N2333/986

摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

摘要翻译： 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品中存在β-内酰胺酶的方法，例如作为包括编码β-内酰胺酶的序列的核酸序列的表达的指示剂。 公开了包括公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-内酰胺酶或在测定中使用组分的说明书。

公开(公告)号：US20080038772A1

公开(公告)日：2008-02-14

申请号：US11873185

申请日：2007-10-16

申请人： Brian Filanoski ,  I. Greenfield ,  James Hirsch

发明人： Brian Filanoski ,  I. Greenfield ,  James Hirsch

IPC分类号： G01N1/30

CPC分类号： G01N1/30 ,  C07D501/00 ,  G01N2001/302

摘要： Compositions, methods and kits are disclosed for improved staining of a cell or tissue with a dye-conjugate that binds specifically to a particular component of the cell or tissue. The compositions, methods and kits include a polymeric material that reduces non-specific binding of a dye-conjugate to components of the cell or tissue other than the particular component specifically bound by the dye-conjugate. In some embodiments, the polymeric material is a synthetic polymer or a naturally-occurring polymer that is substituted by multiple sulfate, sulfonate, phosphate and/or phosphonate groups.

公开(公告)号：US20080038773A1

公开(公告)日：2008-02-14

申请号：US11873281

申请日：2007-10-16

申请人： Brian Filanoski ,  I. Greenfield ,  James Hirsch

发明人： Brian Filanoski ,  I. Greenfield ,  James Hirsch

IPC分类号： G01N1/30 ,  C12Q1/00

CPC分类号： G01N33/52 ,  G01N1/30

摘要： Compositions, methods and kits are disclosed for improved staining of a cell or tissue with a dye-conjugate that binds specifically to a particular component of the cell or tissue. The compositions, methods and kits include a polymeric material that reduces non-specific binding of a dye-conjugate to components of the cell or tissue other than the particular component specifically bound by the dye-conjugate. In some embodiments, the polymeric material is a synthetic polymer or a naturally-occurring polymer that is substituted by multiple sulfate, sulfonate, phosphate and/or phosphonate groups.

摘要翻译： 公开了组合物，方法和试剂盒，用于改善用特异性结合细胞或组织的特定组分的染料缀合物染色细胞或组织。 组合物，方法和试剂盒包括聚合物材料，其减少染料缀合物与除了由染料缀合物特异结合的特定组分以外的细胞或组织的组分的非特异性结合。 在一些实施方案中，聚合物材料是由多种硫酸盐，磺酸盐，磷酸盐和/或膦酸盐基团取代的合成聚合物或天然存在的聚合物。