COMPOSITION, METHOD AND KIT FOR REDUCING BACKGROUND STAINING

    公开(公告)号:US20080038772A1

    公开(公告)日:2008-02-14

    申请号:US11873185

    申请日:2007-10-16

    IPC分类号: G01N1/30

    摘要: Compositions, methods and kits are disclosed for improved staining of a cell or tissue with a dye-conjugate that binds specifically to a particular component of the cell or tissue. The compositions, methods and kits include a polymeric material that reduces non-specific binding of a dye-conjugate to components of the cell or tissue other than the particular component specifically bound by the dye-conjugate. In some embodiments, the polymeric material is a synthetic polymer or a naturally-occurring polymer that is substituted by multiple sulfate, sulfonate, phosphate and/or phosphonate groups.

    COMPOSITION, METHOD AND KIT FOR REDUCING BACKGROUND STAINING
    7.
    发明申请
    COMPOSITION, METHOD AND KIT FOR REDUCING BACKGROUND STAINING 审中-公开
    用于减少背景染色的组合物,方法和工具包

    公开(公告)号:US20080038773A1

    公开(公告)日:2008-02-14

    申请号:US11873281

    申请日:2007-10-16

    IPC分类号: G01N1/30 C12Q1/00

    CPC分类号: G01N33/52 G01N1/30

    摘要: Compositions, methods and kits are disclosed for improved staining of a cell or tissue with a dye-conjugate that binds specifically to a particular component of the cell or tissue. The compositions, methods and kits include a polymeric material that reduces non-specific binding of a dye-conjugate to components of the cell or tissue other than the particular component specifically bound by the dye-conjugate. In some embodiments, the polymeric material is a synthetic polymer or a naturally-occurring polymer that is substituted by multiple sulfate, sulfonate, phosphate and/or phosphonate groups.

    摘要翻译: 公开了组合物,方法和试剂盒,用于改善用特异性结合细胞或组织的特定组分的染料缀合物染色细胞或组织。 组合物,方法和试剂盒包括聚合物材料,其减少染料缀合物与除了由染料缀合物特异结合的特定组分以外的细胞或组织的组分的非特异性结合。 在一些实施方案中,聚合物材料是由多种硫酸盐,磺酸盐,磷酸盐和/或膦酸盐基团取代的合成聚合物或天然存在的聚合物。