公开(公告)号：US20140162281A1

公开(公告)日：2014-06-12

申请号：US13868824

申请日：2013-04-23

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Kyung-yeon HAN ,  Yeon-jeong KIM ,  Jong-myeon PARK ,  Chang-eun YOO

IPC: G01N1/34

CPC classification number: G01N1/34 ,  G01N33/54326 ,  G01N33/574

Abstract: Provided are compositions, kits, and methods for separating cells including complexes of at least one type of linker capable of binding to an antibody or antigen binding fragment and a solid phase.

Abstract translation: 提供了用于分离细胞的组合物，试剂盒和方法，包括能够结合抗体或抗原结合片段和固相的至少一种类型连接体的复合物。

公开(公告)号：US20150044725A1

公开(公告)日：2015-02-12

申请号：US14222314

申请日：2014-03-21

Applicant: Samsung Electronics Co., Ltd.

Inventor： Chang-eun YOO ,  Yeon-jeong Kim ,  Dong-hyun Park ,  Kyung-yeon Han

IPC: C12Q1/68

CPC classification number: C12N15/1013 ,  C12N15/1006

Abstract: A method of separating nucleic acids from cells, the method comprising incubating a sample comprising cells with a solid substrate that binds to the cells, whereby the cells adhere to the solid substrate; suspending the solid substrate adhered to the cells in a lysis composition comprising about 100 mM to about 300 mM of alkaline metal salt, and having a pH of about 6 to about 8; lysing the cells in the lysis composition to obtain a lysed solution; and obtaining the nucleic acids from the lysed solution; as well as related compositions and kits.

Abstract translation: 一种从细胞中分离核酸的方法，所述方法包括将包含细胞的样品与固定于细胞上的固体底物孵育，由此所述细胞粘附到固体基质上; 将固体底物悬浮在包含约100mM至约300mM碱金属盐并且pH为约6至约8的裂解组合物中的细胞上; 裂解裂解组合物中的细胞以获得裂解溶液; 从裂解的溶液中获得核酸; 以及相关组合物和试剂盒。

公开(公告)号：US20140093880A1

公开(公告)日：2014-04-03

申请号：US13857087

申请日：2013-04-04

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Ga-hee KIM ,  Chang-eun YOO ,  Ye-ryoung YONG ,  Myo-yong LEE

IPC: G01N1/40 ,  C12Q1/68 ,  G01N33/574

CPC classification number: G01N1/405 ,  C12Q1/6886 ,  G01N33/5076 ,  G01N33/57492

Abstract: A composition, a kit, and a method of isolating a vesicle from a sample using a compound comprising zwitterion moieties, which may be used to analyze vesicles, and proteins, glycoprotein, lipids, or nucleic acids thereof.

Abstract translation: 使用可用于分析囊泡的包含两性离子部分的化合物和蛋白质，糖蛋白，脂质或其核酸的组合物，试剂盒和从样品中分离囊泡的方法。

公开(公告)号：US20130302856A1

公开(公告)日：2013-11-14

申请号：US13768891

申请日：2013-02-15

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Chang-eun YOO ,  Ga-hee KIM ,  Myoung-soon KIM

IPC: C07H1/08

CPC classification number: C07H1/08 ,  C12N15/111 ,  C12N2310/141 ,  C12N2330/50 ,  C12Q1/6851 ,  C12Q2600/178

Abstract: A method of extracting a nucleic acid from a microvesicle, the method comprising treating the microvesicle with a composition comprising a detergent and an aprotic solvent to extract a nucleic acid from the microvesicle.

Abstract translation: 一种从微泡中提取核酸的方法，所述方法包括用包含洗涤剂和非质子溶剂的组合物处理微泡，以从微泡中提取核酸。

公开(公告)号：US20130302822A1

公开(公告)日：2013-11-14

申请号：US13768680

申请日：2013-02-15

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Myoung-soon KIM ,  Dong-hyun PARK ,  Chang-eun YOO ,  Ga-hee KIM

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N33/56966 ,  G01N2333/70596

Abstract: A method of analyzing exosomes, the method comprising incubating a sample comprising fluorescence-labeled exosomes with a solid support so that the fluorescence-labeled exosomes bind to the solid support; measuring a fluorescence signal from the bound fluorescence-labeled exosomes; and analyzing the exosomes based on the measured fluorescence signal.

Abstract translation: 一种分析外来体的方法，所述方法包括将包含荧光标记的外来体的样品与固体支持物孵育，使得荧光标记的外来体与固体支持物结合; 从结合的荧光标记的外来体测量荧光信号; 并根据测量的荧光信号分析外来体。