知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

25 条记录 (耗时 0.027 秒)

    Polymerases engineered to reduce nucleotide-independent DNA binding
    2.
    发明授权
    Polymerases engineered to reduce nucleotide-independent DNA binding 有权

    公开(公告)号：US10597643B2

    公开(公告)日：2020-03-24

    申请号：US15866353

    申请日：2018-01-09

    申请人： OMNIOME, INC.

    发明人： Pinar Iyidogan ,  Mark C. Wallen ,  Ying L. Liu ,  Kandaswamy Vijayan

    IPC分类号： C12N9/12 ,  C12Q1/6869

    摘要： Provided are engineered DNA polymerases exhibiting modified functionality, and polynucleotides encoding same. Modified features include: (1) reduced catalytic activity in the presence of magnesium ions and/or (2) reduced affinity for primed template nucleic acid molecules in the absence of cognate nucleotide, and an ability to discriminate between cognate and non-cognate nucleotides under low salt conditions. Sequencing By Binding™ procedures employing the engineered polymerases have certain advantages. The engineered polymerases can have other uses as well.

    Methods and apparatus that increase sequencing-by-binding efficiency
    3.
    发明授权
    Methods and apparatus that increase sequencing-by-binding efficiency 有权

    公开(公告)号：US10161003B2

    公开(公告)日：2018-12-25

    申请号：US15922787

    申请日：2018-03-15

    申请人： OMNIOME, INC.

    发明人： Sean Stromberg ,  John Vieceli ,  Kandaswamy Vijayan ,  Arnold Oliphant

    IPC分类号： C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6853

    摘要： A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence of ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the next template position from an error in the base call.

    METHOD OF NUCLEIC ACID SEQUENCE DETERMINATION
    5.
    发明申请
    METHOD OF NUCLEIC ACID SEQUENCE DETERMINATION 审中-公开

    公开(公告)号：US20170314072A1

    公开(公告)日：2017-11-02

    申请号：US15581822

    申请日：2017-04-28

    申请人： Omniome, Inc.

    发明人： Kandaswamy Vijayan ,  Pinar Iyidogan

    IPC分类号： C12Q1/68 ,  C12N9/12

    摘要： Provided are sequencing-by-binding methods of detecting cognate nucleotides using a crippled DNA polymerizing enzyme that possesses the ability to bind the next correct nucleotide downstream of a primer in a template-dependent fashion, but does not possess the activity needed to promote phosphodiester bond formation. Use of the crippled DNA polymerase permits interrogation of one nucleotide at a time, without incorporation of any nucleotide. Labeled nucleotides, such as fluorescently labeled nucleotides, can be used in conjunction with the crippled DNA polymerase to establish cognate nucleotide identity in a rapid manner.

    Methods and apparatus that increase sequencing-by-binding efficiency
    6.
    发明授权
    Methods and apparatus that increase sequencing-by-binding efficiency 有权

    公开(公告)号：US09951385B1

    公开(公告)日：2018-04-24

    申请号：US15712632

    申请日：2017-09-22

    申请人： OMNIOME, INC.

    发明人： Kandaswamy Vijayan ,  Arnold Oliphant

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q2525/186 ,  C12Q2565/101

    摘要： A method of nucleic acid detection including (a) contacting a primed template with a polymerase, nucleotide cognate of a first base type and nucleotide cognate of a second base type under ternary complex stabilizing conditions; (b) contacting the primed template nucleic acid with a polymerase, nucleotide cognate of the first base type and nucleotide cognate of a third base type under ternary complex stabilizing conditions; (c) examining products of (a) and (b) for signals produced by a ternary complex that includes the primed template nucleic acid, polymerase and next correct nucleotide, wherein signals for the product of (a) are ambiguous for the first and second base type, and signals for the product of (b) are ambiguous for the first and third base type; (d) disambiguating signals acquired in (c) to identify the next correct nucleotide.

    NUCLEIC ACID SEQUENCING METHODS AND SYSTEMS
    7.
    发明申请
    NUCLEIC ACID SEQUENCING METHODS AND SYSTEMS 审中-公开
    核酸序列测序方法和系统

    公开(公告)号：US20170022553A1

    公开(公告)日：2017-01-26

    申请号：US14805381

    申请日：2015-07-21

    申请人： Omniome, Inc.

    发明人： Kandaswamy Vijayan ,  Eugene Tu ,  Mark A. Bernard

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2563/103 ,  C12Q2565/607

    摘要： The present disclosure provides compositions, methods and systems for sequencing a template nucleic acid using a polymerase based, nucleic acid binding reaction involving examination of the interaction between a polymerase and template nucleic acid in the presence of one or more unlabeled nucleotides. The methods rely, in part, on identifying a base of a template nucleic acid during nucleic acid synthesis by controlling the sequencing reaction conditions. Template nucleic acid bases may be identified during an examination step followed by an optional incorporation step.

    摘要翻译： 本公开提供了使用基于聚合酶的核酸结合反应来测序模板核酸的组合物，方法和系统，所述核酸结合反应涉及在一个或多个未标记的核苷酸存在下检测聚合酶和模板核酸之间的相互作用。 该方法部分地依赖于通过控制测序反应条件在核酸合成中鉴定模板核酸的碱基。 模板核酸碱基可以在检查步骤之后，然后是任选的掺入步骤。