- Patent Title: Polymerases engineered to reduce nucleotide-independent DNA binding
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Application No.: US15866353Application Date: 2018-01-09
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Publication No.: US10597643B2Publication Date: 2020-03-24
- Inventor: Pinar Iyidogan , Mark C. Wallen , Ying L. Liu , Kandaswamy Vijayan
- Applicant: OMNIOME, INC.
- Applicant Address: US CA San Diego
- Assignee: OMNIOME, INC.
- Current Assignee: OMNIOME, INC.
- Current Assignee Address: US CA San Diego
- Agency: Kilpatrick Townsend & Stockton LLP
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12Q1/6869

Abstract:
Provided are engineered DNA polymerases exhibiting modified functionality, and polynucleotides encoding same. Modified features include: (1) reduced catalytic activity in the presence of magnesium ions and/or (2) reduced affinity for primed template nucleic acid molecules in the absence of cognate nucleotide, and an ability to discriminate between cognate and non-cognate nucleotides under low salt conditions. Sequencing By Binding™ procedures employing the engineered polymerases have certain advantages. The engineered polymerases can have other uses as well.
Public/Granted literature
- US20180155698A1 POLYMERASES ENGINEERED TO REDUCE NUCLEOTIDE-INDEPENDENT DNA BINDING Public/Granted day:2018-06-07
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