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公开(公告)号：US20160356739A1
公开(公告)日：2016-12-08
申请号：US15238795
申请日：2016-08-17
申请人： Mohammad Abdolahad , Ali Saeidi , Milad Gharooni
发明人： Mohammad Abdolahad , Ali Saeidi , Milad Gharooni
IPC分类号： G01N27/327 , G01N27/28 , C23C28/00 , C23C14/30 , C23C14/14 , C23C16/24 , G01N33/483 , C25F3/08
摘要： An electrical probe configured to measure an electrical response from a biological cell includes a microwire having a sharpened tip, a catalyst layer formed on the sharpened tip of the microwire, and an array of nanotube electrodes vertically aligned on the catalyst layer.
摘要翻译： 构造成测量来自生物细胞的电响应的电探针包括具有尖锐尖端的微线，形成在微线的尖锐尖端上的催化剂层和在催化剂层上垂直对准的纳米管电极阵列。
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公开(公告)号：US20160356761A1
公开(公告)日：2016-12-08
申请号：US15238917
申请日：2016-08-17
申请人： Mohammad Abdolahad , Ali Saeidi , Milad Gharooni
发明人： Mohammad Abdolahad , Ali Saeidi , Milad Gharooni
IPC分类号： G01N33/483 , G01N27/02
CPC分类号： G01N27/3278 , C23C14/16 , C23C28/321 , C23C28/322 , C23C28/34 , C25F3/08 , G01N27/028 , G01N27/283 , G01N33/4833
摘要： A single-cell-based electromechanical method for cancerous state detection including the steps of preparing a suspension of individually suspended biological cells, extracting a single cell from the suspension, holding the extracted single cell from the suspension, measuring a first electrical response of the held single cell, step-wised mechanical aspirating the held single cell to form a mechanically deformed cell, and measuring an electrical response of the held single cell after each step of mechanical aspirating. The cancerous state of the single cell is determined based on the changes in the measured electrical responses.
摘要翻译： 一种用于癌症状态检测的基于单细胞的机电方法，包括以下步骤：制备单独悬浮的生物细胞的悬浮液，从悬浮液中提取单细胞，将悬浮液中提取的单细胞保持，测量所保持的 单细胞，步进式机械抽吸所保持的单细胞以形成机械变形的细胞，并且在机械抽吸的每个步骤之后测量所保持的单细胞的电响应。 基于测量的电响应的变化确定单细胞的癌症状态。
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公开(公告)号：US10775336B2
公开(公告)日：2020-09-15
申请号：US15238795
申请日：2016-08-17
申请人： Mohammad Abdolahad , Ali Saeidi , Milad Gharooni
发明人： Mohammad Abdolahad , Ali Saeidi , Milad Gharooni
IPC分类号： G01N27/327 , G01N33/483 , G01N27/28 , C25F3/08 , C23C28/00 , C23C14/16 , G01N27/02
摘要： An electrical probe is disclosed for measuring an electrical response from a biological cell. The electrical probe includes a tungsten microwire having a sharpened tip section, a catalyst layer formed on the sharpened tip section of the tungsten microwire, and an array of nanotube electrodes vertically aligned on the catalyst layer. The catalyst layer includes a catalyst bilayer including a nickel layer over a gold layer, and the nanotube electrodes include a plurality of silicon nanotubes (SiNTs).
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公开(公告)号：US20170269087A1
公开(公告)日：2017-09-21
申请号：US15616154
申请日：2017-06-07
申请人： Mohammad Abdolahad , MOHAMMAD SAEID NIK SHOAR , Milad Gharooni , MOHAMMADALI KHAYAMIAN , FARSHAD REZAKHANLOO , SAEED ANSARIAN
发明人： Mohammad Abdolahad , MOHAMMAD SAEID NIK SHOAR , Milad Gharooni , MOHAMMADALI KHAYAMIAN , FARSHAD REZAKHANLOO , SAEED ANSARIAN
IPC分类号： G01N33/574 , C12N5/071 , G06F19/00 , G01N33/50 , C12Q1/68
CPC分类号： G01N33/574 , C12N5/0602 , C12Q1/6886 , G01N33/5005 , G01N33/5044 , G01N2333/46 , G01N2800/56 , G01N2800/7028 , G06F19/00
摘要： A method for metastasis diagnosis, including adhering a plurality of Human Umbilical Vein Endothelial Cells (HUVECs) on an array of electrodes patterned on a substrate to cover the array of electrodes by HUVECs, measuring an initial electrical signal from each electrode of the array of electrodes, introducing a metastatic-suspicious sample onto the substrate and measuring a set of time-lapse electrical signals from the array of electrodes. Each electrode has an On/Off two-state, including an On state for an entirely-covered electrode by a HUVEC and an Off state for a partially-covered electrode by a HUVEC. Diagnosing metastasis responsive to detecting a state change from On to Off for at least one electrode of the array of electrodes.
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公开(公告)号：US10761081B2
公开(公告)日：2020-09-01
申请号：US15616054
申请日：2017-06-07
申请人： Mohammad Abdolahad , Mohammad Saeid Nik Shoar , Milad Gharooni , Mohammadali Khayamian , Farshad Rezakhanloo , Saeed Ansarian
发明人： Mohammad Abdolahad , Mohammad Saeid Nik Shoar , Milad Gharooni , Mohammadali Khayamian , Farshad Rezakhanloo , Saeed Ansarian
IPC分类号： G01N33/487 , G01N33/574 , G01N33/483 , G01N27/04 , G01N33/50 , G01N33/543 , G01N23/225
摘要： A system for diagnosing metastasis in a metastatic-suspicious sample, including: a bio-chip, an electrical signal extraction board and a processor. The bio-chip includes a biosensor including a substrate, an array of electrodes, where the array of electrodes is patterned on the substrate and at least one Human Umbilical Vein Endothelial Cell (HUVEC) which is adhered on the substrate and on the array of electrodes forming a biological trap for a metastatic cell. A metastatic-suspicious sample may be introduced onto the bio-chip allowing for diagnosis of metastasis by monitoring the time-lapse electrical signals that are recorded by the processor.
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公开(公告)号：US09869666B2
公开(公告)日：2018-01-16
申请号：US15058909
申请日：2016-03-02
IPC分类号： C12Q1/02 , C12Q1/00 , G01N33/483 , H01L21/02 , G01N27/02 , G01N33/50 , G01N33/574 , G01N27/327
CPC分类号： G01N33/4836 , G01N27/02 , G01N27/021 , G01N27/3278 , G01N33/5011 , G01N33/5044 , G01N33/574 , H01L21/02381 , H01L21/02488 , H01L21/02532 , H01L21/02603 , H01L21/02645 , H01L21/02653
摘要： A method for detection and monitoring a spreading stage of a biological cell for cancer diagnosis is disclosed. The method includes steps of removing biological cell lines from a material; culturing the cell lines via maintaining the removed biological cell lines in an appropriate medium at a controlled set of conditions; seeding the cultured biological cells lines on silicon nanowire electrode arrays of an electrical cell-substrate impedance sensor (ECIS); and measuring an electrical impedance of the seeded biological cell lines to detect and monitor a spreading state of the seeded biological cell lines for cancer diagnosis.
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公开(公告)号：US20180149652A1
公开(公告)日：2018-05-31
申请号：US15871486
申请日：2018-01-15
IPC分类号： G01N33/574 , C12Q1/6825 , G01N27/403 , G01N27/02 , G01N27/327 , G01N33/483 , G01N33/50 , G01N33/543 , A61B5/1477
CPC分类号： G01N33/574 , A61B5/1477 , C12Q1/6825 , G01N27/021 , G01N27/3278 , G01N27/403 , G01N33/4836 , G01N33/5044 , G01N33/5091 , G01N33/54373
摘要： A method for detection and monitoring a spreading stage of a biological cell for cancer diagnosis is disclosed. The method includes steps of removing biological cell lines from a material; culturing the cell lines via maintaining the removed biological cell lines in an appropriate medium at a controlled set of conditions; seeding the cultured biological cells lines on silicon nanowire electrode arrays of an electrical cell-substrate impedance sensor (ECIS); and measuring an electrical impedance of the seeded biological cell lines to detect and monitor a spreading state of the seeded biological cell lines for cancer diagnosis.
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公开(公告)号：US20180100849A1
公开(公告)日：2018-04-12
申请号：US15616054
申请日：2017-06-07
申请人： Mohammad Abdolahad , MOHAMMAD SAEID NIK SHOAR , Milad Gharooni , MOHAMMADALI KHAYAMIAN , FARSHAD REZAKHANLOO , SAEED ANSARIAN
发明人： Mohammad Abdolahad , MOHAMMAD SAEID NIK SHOAR , Milad Gharooni , MOHAMMADALI KHAYAMIAN , FARSHAD REZAKHANLOO , SAEED ANSARIAN
IPC分类号： G01N33/487 , G01N33/574 , G01N33/483 , G01N27/04
CPC分类号： G01N33/48728 , G01N23/225 , G01N27/045 , G01N33/4836 , G01N33/48792 , G01N33/5044 , G01N33/5438 , G01N33/574 , G01N2800/56
摘要： A system for diagnosing metastasis in a metastatic-suspicious sample, including: a bio-chip, an electrical signal extraction board and a processor. The bio-chip includes a biosensor including a substrate, an array of electrodes, where the array of electrodes is patterned on the substrate and at least one Human Umbilical Vein Endothelial Cell (HUVEC) which is adhered on the substrate and on the array of electrodes forming a biological trap for a metastatic cell. A metastatic-suspicious sample may be introduced onto the bio-chip allowing for diagnosis of metastasis by monitoring the time-lapse electrical signals that are recorded by the processor.
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公开(公告)号：US20160178613A1
公开(公告)日：2016-06-23
申请号：US15058922
申请日：2016-03-02
CPC分类号： G01N33/4836 , G01N27/02 , G01N27/021 , G01N27/3278 , G01N33/5011 , G01N33/5044 , G01N33/574 , H01L21/02381 , H01L21/02488 , H01L21/02532 , H01L21/02603 , H01L21/02645 , H01L21/02653
摘要： A method for detection and monitoring a therapeutic effect of a cancer treatment drug is disclosed. The method includes steps of removing a malignant biological cell lines from a tumor; culturing the removed biological cell lines in a controlled set of conditions; seeding the cultured biological cell lines on silicon nanowire electrode arrays of an electrical cell-substrate impedance sensor (ECIS); adding a cancer treatment drug to the seeded biological cell lines to treat the seeded biological cell lines; and measuring an electrical impedance of the treated biological cell lines for detection and monitoring a therapeutic effect of the cancer treatment drug.
摘要翻译： 公开了一种用于检测和监测癌症治疗药物的治疗效果的方法。 该方法包括从肿瘤中去除恶性生物细胞系的步骤; 在受控的一组条件下培养去除的生物细胞系; 将培养的生物细胞系接种在电池 - 基底阻抗传感器（ECIS）的硅纳米线电极阵列上; 向接种的生物细胞系添加癌症治疗药物以处理接种的生物细胞系; 并测量经处理的生物细胞系的电阻抗，用于检测和监测癌症治疗药物的治疗效果。
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10.发明申请公开(公告)号：US20180313812A1
公开(公告)日：2018-11-01
申请号：US16030066
申请日：2018-07-09
IPC分类号： G01N33/483 , G01N27/02
摘要： A method for detecting a metastasis state of biological cells is disclosed. The method includes seeding a plurality of biological cells onto an array of electrodes of an electrical cell-substrate impedance sensor (ECIS) by dropping a cell suspension including the plurality of biological cells in a cell culture medium onto the array of electrodes, forming a plurality of cultured biological cells attached onto the array of electrodes by maintaining the ECIS in an incubator, reducing pH value of an extracellular media around the plurality of cultured biological cells to a pH value between 6.2 and 6.7 by dropping an acidic solution onto the array of electrodes, activating an intracellular phenomenon due to reducing pH value of the extracellular media around the plurality of cultured biological cells, monitoring an electrical signal of the plurality of cultured biological cells for a pre-determined period of time, and determining metastasis state of the plurality of biological cells based on the monitored electrical signals. The intracellular phenomenon includes one of autophagy phenomenon in metastatic cells, or cell's proliferation reduction and/or apoptosis in non-metastatic cells. Monitoring the electrical signal of the plurality of cultured biological cells includes applying an electrical voltage to the array of electrodes and extracting a set of time-lapse electrical signals from the array of electrodes. Determining metastasis state of the plurality of biological cells includes identifying a metastatic state for the plurality of biological cells by detecting an increasing trend in the set of time-lapse electrical signals over time, where the increasing trend occurs responsive to activation of the autophagy phenomenon. Determining metastasis state of the plurality of biological cells further includes identifying a non-metastatic state for the plurality of biological cells by detecting a decreasing trend in the set of time-lapse electrical signals over time, where the decreasing trend occurs responsive to activation of the cell's proliferation reduction and/or apoptosis phenomenon.
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