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公开(公告)号：US20210239452A1
公开(公告)日：2021-08-05
申请号：US17234982
申请日：2021-04-20
发明人： Johann Engelhardt
摘要： In a method of detecting changes in direction of a collimated coherent light beam, the light beam is split into partial light beams which are superimposed on a camera to form an interference pattern displaying light intensity minima and maxima alternatingly following to one another in a transverse direction oriented transversely to an average propagation direction of the partial beams. The light beam is focused into at least one focus located in front of the camera. Pictures of the interference pattern including a plurality of the light intensity maxima are registered with the camera. An average shift of the plurality of light intensity maxima with regard to the camera in the at least one transverse direction is determined from the pictures. A change in angular orientation of the collimated coherent light beam in the at least one transvers direction is deduced from the average shift.
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2.发明申请STED Microscopy With Pulsed Excitation, Continuous Stimulation, And Gated Registration Of Spontaneously Emitted Fluorescence Light 有权具有脉冲激发，连续刺激和自发发射荧光灯门控的STED显微镜公开(公告)号：US20130256564A1
公开(公告)日：2013-10-03
申请号：US13899938
申请日：2013-05-22
申请人： Deutsches Krebsforschungszentrum , Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
发明人： Stefan W. Hell , Johann Engelhardt , Matthias Reuss , Volker Westphal , Christian Eggeling , Gael Moneron , Kyu-Young Han , Giuseppe Vicidomini , Katrin Willig
IPC分类号： G01N21/64
CPC分类号： G01N21/64 , G01N21/636 , G01N21/6408 , G01N21/6458 , G02B21/0076 , G02B21/16
摘要： In a STED fluorescence light microscope pulses of excitation light (3) are applied to a sample, which excite fluorescent entities contained in the sample for fluorescence, and which are focused on at least one focal area. Further, de-excitation light (12) is applied to the sample, which de-excites the excited fluorescent entities and which comprises an intensity zero point in the at least one focal area, as a continuous wave. Fluorescence light emitted by the excited fluorescent entities in the sample is registered after each pulse of the excitation light (3) and overlapping with applying the de-excitation light (13) with high temporal resolution between consecutive pulses of the excitation light (3).
摘要翻译： 在STED荧光光学显微镜中，将激发光（3）的脉冲施加到样品上，该样品激发样品中包含的荧光实体用于荧光，并且聚焦在至少一个焦点区域上。 此外，将去激发光（12）施加到样品，其将激发的荧光实体去激发，并且其在至少一个焦点区域中包括强度零点，作为连续波。 在激发光（3）的每个脉冲之后，记录被激发的荧光体在样品中发出的荧光，并且在激发光（3）的连续脉冲之间以高时间分辨率施加去激发光（13）重叠。
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公开(公告)号：US12104902B2
公开(公告)日：2024-10-01
申请号：US17234982
申请日：2021-04-20
发明人： Johann Engelhardt
CPC分类号： G01B9/02098 , G01B9/02041 , G01B11/26 , G02B5/04 , G02B21/0036 , G02B21/008 , G06T7/33 , G06T7/74
摘要： In a method of detecting changes in direction of a collimated coherent light beam, the light beam is split into partial light beams which are superimposed on a camera to form an interference pattern displaying light intensity minima and maxima alternatingly following to one another in a transverse direction oriented transversely to an average propagation direction of the partial beams. The light beam is focused into at least one focus located in front of the camera. Pictures of the interference pattern including a plurality of the light intensity maxima are registered with the camera. An average shift of the plurality of light intensity maxima with regard to the camera in the at least one transverse direction is determined from the pictures. A change in angular orientation of the collimated coherent light beam in the at least one transvers direction is deduced from the average shift.
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公开(公告)号：US11754847B2
公开(公告)日：2023-09-12
申请号：US17209516
申请日：2021-03-23
发明人： Johann Engelhardt , Stefan W. Hell
CPC分类号： G02B27/0927 , G01N21/6402 , G01N21/6458 , G02B21/06 , G02B21/16 , G02B27/0994 , G02B27/106 , G02F1/035 , G02F2203/50
摘要： For forming and shifting a light intensity distribution in a focal area of an objective lens, portions of coherent input light are one by one directed into non-identical two-dimensional pupil areas of a pupil of the objective lens. Each of the portions of coherent input light is collimated in the pupil. The pupil areas include a pair of two pupil areas which are axially symmetrically arranged on opposite sides of an optical axis of the objective lens. At least one of the two discrete portions of coherent input light that are directed into the pair of pupil areas is separately modulated with regard to its phase by means of an electro optical modulator such as to form the light intensity distribution in the focal area with a local intensity minimum delimited by intensity maxima and to shift the local intensity minimum laterally with regard to the optical axis.
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公开(公告)号：US11513327B2
公开(公告)日：2022-11-29
申请号：US16896502
申请日：2020-06-09
申请人： Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V. , Deutsches Krebsforschungszentrum
发明人： Johann Engelhardt , Jonas Marquard
IPC分类号： G02B21/00
摘要： Upstream a microscope objective lens, a polarization direction of a light beam is tilted with a first electro-optical deflector between a first polarization direction with which the light beam is deflected by a first polarization beam splitter by a first angle and a second polarization direction with which it is deflected by a second angle. With a second electro-optical deflector, the polarization direction of the light beam is tilted between a third polarization direction with which the light beam is deflected by a second polarization beam splitter by a third angle and a fourth polarization direction with which it is deflected by a fourth angle. By rotating the polarization direction of the light beam by means of the first and second electro-optical deflectors in a coordinated way the light beam is tilted about a fixed point in a pupil of the objective lens.
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公开(公告)号：US20210208411A1
公开(公告)日：2021-07-08
申请号：US17209516
申请日：2021-03-23
发明人： Johann Engelhardt , Stefan W. Hell
摘要： For forming and shifting a light intensity distribution in a focal area of an objective lens, portions of coherent input light are one by one directed into non-identical two-dimensional pupil areas of a pupil of the objective lens. Each of the portions of coherent input light is collimated in the pupil. The pupil areas include a pair of two pupil areas which are axially symmetrically arranged on opposite sides of an optical axis of the objective lens. At least one of the two discrete portions of coherent input light that are directed into the pair of pupil areas is separately modulated with regard to its phase by means of an electro optical modulator such as to form the light intensity distribution in the focal area with a local intensity minimum delimited by intensity maxima and to shift the local intensity minimum laterally with regard to the optical axis.
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7.发明申请公开(公告)号：US20200310094A1
公开(公告)日：2020-10-01
申请号：US16896502
申请日：2020-06-09
申请人： Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V. , Deutsches Krebsforschungszentrum
发明人： Johann Engelhardt , Jonas Marquard
IPC分类号： G02B21/00
摘要： Upstream a microscope objective lens, a polarization direction of a light beam is tilted with a first electro-optical deflector between a first polarization direction with which the light beam is deflected by a first polarization beam splitter by a first angle and a second polarization direction with which it is deflected by a second angle. With a second electro-optical deflector, the polarization direction of the light beam is tilted between a third polarization direction with which the light beam is deflected by a second polarization beam splitter by a third angle and a fourth polarization direction with which it is deflected by a fourth angle. By rotating the polarization direction of the light beam by means of the first and second electro-optical deflectors in a coordinated way the light beam is tilted about a fixed point in a pupil of the objective lens.
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8.发明授权STED microscopy with pulsed excitation, continuous stimulation, and gated registration of spontaneously emitted fluorescence light 有权具有脉冲激发，连续刺激和自发发射荧光的门控对照的STED显微镜公开(公告)号：US09551658B2
公开(公告)日：2017-01-24
申请号：US13899938
申请日：2013-05-22
申请人： Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V. , Deutsches Krebsforschungszentrum
发明人： Stefan W. Hell , Johann Engelhardt , Matthias Reuss , Volker Westphal , Christian Eggeling , Gael Moneron , Kyu-Young Han , Giuseppe Vicidomini , Katrin Willig
CPC分类号： G01N21/64 , G01N21/636 , G01N21/6408 , G01N21/6458 , G02B21/0076 , G02B21/16
摘要： In a STED fluorescence light microscope pulses of excitation light (3) are applied to a sample, which excite fluorescent entities contained in the sample for fluorescence, and which are focused on at least one focal area. Further, de-excitation light (12) is applied to the sample, which de-excites the excited fluorescent entities and which comprises an intensity zero point in the at least one focal area, as a continuous wave. Fluorescence light emitted by the excited fluorescent entities in the sample is registered after each pulse of the excitation light (3) and overlapping with applying the de-excitation light (13) with high temporal resolution between consecutive pulses of the excitation light (3).
摘要翻译： 在STED荧光光学显微镜中，将激发光（3）的脉冲施加到样品上，该样品激发样品中包含的荧光实体用于荧光，并且聚焦在至少一个焦点区域上。 此外，将去激发光（12）施加到样品，其将激发的荧光实体去激发，并且其在至少一个焦点区域中包括强度零点，作为连续波。 在激发光（3）的每个脉冲之后，记录被激发的荧光体在样品中发出的荧光，并且在激发光（3）的连续脉冲之间以高时间分辨率施加去激发光（13）重叠。
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9.发明申请公开(公告)号：US20140097358A1
公开(公告)日：2014-04-10
申请号：US14102758
申请日：2013-12-11
申请人： Deutsches Krebsforschungszentrum , Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
IPC分类号： G01N21/64
CPC分类号： G01N21/64 , G01N21/6458 , G02B21/16 , G02B21/365
摘要： In a method for imaging a structure (33) marked with a fluorescent dye in a sample, the sample is repeatedly scanned in a scanning range (28) with a light intensity distribution localised around a focal point (29) of a focused fluorescence excitation light beam. The light intensity distribution further comprises a focused fluorescence inhibiting light beam (7) whose wave fronts are modulated so that a fluorescence inhibiting light intensity distribution comprises a minimum at the focal point (29) of the fluorescence excitation light beam (4). The scanning conditions are coordinated in such a way that the fluorescence light is emitted out of the scanning range (28) as individually detectable photons. When these photons are detected, the location (32) of the focal point (28) at the respective point in time is allocated to them. An image of the structure (33) is composed of the locations (32) to which the detected photons have been allocated during several repetitions of scanning the scanning range (28).
摘要翻译： 在用于对在样品中标记有荧光染料的结构（33）进行成像的方法中，在扫描范围（28）中重复扫描样品，其中光强度分布位于聚焦荧光激发光的焦点（29）周围 光束。 光强度分布还包括其波前被调制的聚焦荧光抑制光束（7），使得荧光抑制光强度分布在荧光激发光束（4）的焦点（29）处包含最小值。 扫描条件以这样的方式协调，使得荧光作为单独检测的光子发射出扫描范围（28）。 当检测到这些光子时，将焦点（28）在相应时间点的位置（32）分配给它们。 结构（33）的图像由在扫描范围（28）的多次重复期间被检测到的光子已被分配的位置（32）组成。
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