公开(公告)号：US20160363537A1

公开(公告)日：2016-12-15

申请号：US15037455

申请日：2014-11-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI ,  Volker SEYFRIED ,  William C. HAY

IPC: G01N21/65 ,  G01J3/10 ,  G01J3/44

CPC classification number: G01N21/65 ,  G01J3/0227 ,  G01J3/10 ,  G01J3/44 ,  G01N2021/655

Abstract: A Raman microscopy imaging device (100) is described, having: a first laser light source (12) for emitting a first laser beam (16) having a first wavelength along a first light path (20); a second laser light source (44) for emitting a second laser beam (18) having a second wavelength, different from the first wavelength, along a second light path (22) physically separated from the first light path (20); a beam combining element (32) for collinearly combining the two laser beams (16, 18) in one shared light path (34) directed onto a sample; a detector (38) for sensing a measured signal on the basis of the two laser beams (16, 18) interacting with the sample; and an evaluation unit (40) for evaluating the measured signal sensed by the detector (38). According to the present invention the first laser light source (12) is embodied as a pulsed source, and the second laser light source (44) as a continuous source.

Abstract translation: 描述了一种拉曼显微镜成像装置（100），其具有：用于沿第一光路（20）发射具有第一波长的第一激光束（16）的第一激光光源（12）。 第二激光源（44），沿着与第一光路（20）物理分离的第二光路（22）发射具有与第一波长不同的第二波长的第二激光束（18）。 用于在指向样本的一个共享光路（34）中共同组合两个激光束（16,18）的光束组合元件（32） 检测器（38），用于基于与样本相互作用的两个激光束（16,18）感测测量信号; 以及用于评估由检测器（38）感测的测量信号的评估单元（40）。 根据本发明，第一激光源（12）被实现为脉冲源，第二激光源（44）作为连续源。

公开(公告)号：US20140218783A1

公开(公告)日：2014-08-07

申请号：US14249872

申请日：2014-04-10

Applicant: LEICA MICROSYSTEMS CMS GmbH

Inventor： Hilmar GUGEL ,  Volker SEYFRIED

IPC: G02F1/11

CPC classification number: G02F1/116 ,  G02B3/0087 ,  G02B21/0032 ,  G02B21/0064 ,  G02B21/16 ,  G02B27/0927 ,  G02B27/42 ,  G02B27/46 ,  G02F1/00 ,  G02F1/01 ,  G02F1/03 ,  G02F1/11 ,  G02F1/29 ,  G02F1/33 ,  G02F1/353 ,  G02F2201/305 ,  G02F2202/32 ,  G02F2203/12 ,  G02F2203/18 ,  G02F2203/24

Abstract: An acousto-optical filter element (114) is provided which has an acousto-optical crystal (118) having an acoustic signal transmitter (120) for generating acoustic signals in the acousto-optical crystal (118). The acousto-optical crystal (118) is designed to selectively spatially deflect light of a target wavelength from an input light beam (116) entering into the acousto-optical crystal (118), as a function of a high frequency applied to the acoustic signal transmitter (120), and to thereby produce a target light beam (126) having the target wavelength. In addition, the acousto-optical filter element (114) includes a spatial filter element (132) which is located in the target light beam (126) and is designed to selectively suppress the intensity of the target light beam (126) in a plane perpendicular to the propagation direction of the target light beam (126).

Abstract translation: 提供一种声光滤波器元件（114），其具有声光晶体（118），该声光晶体具有用于在声光晶体（118）中产生声信号的声信号发射器（120）。 声光晶体（118）被设计为根据进入声光晶体（118）的输入光束（116）选择性地空间偏转目标波长的光，作为施加到声信号的高频的函数 发射器（120），从而产生具有目标波长的目标光束（126）。 此外，声光滤波器元件（114）包括位于目标光束（126）中的空间滤光元件（132），并被设计成选择性地抑制平面中的目标光束（126）的强度 垂直于目标光束（126）的传播方向。

公开(公告)号：US20160216498A1

公开(公告)日：2016-07-28

申请号：US14916486

申请日：2014-09-03

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Vishnu Vardhan KRISHNAMACHARI ,  Arnold GISKE

IPC: G02B21/00 ,  G02F1/29 ,  G02F1/33 ,  G02B6/26 ,  G02B27/09

CPC classification number: G02B21/0032 ,  G02B6/262 ,  G02B6/32 ,  G02B21/0068 ,  G02B21/0076 ,  G02B27/09 ,  G02B27/0905 ,  G02B27/58 ,  G02F1/292 ,  G02F1/33

Abstract: The invention relates to a microscope having an objective that focuses illuminating light to an illuminating light focus, and having a light-guiding fiber which transports the illuminating light and at whose end is arranged a fiber coupler that couples the illuminating light out of the light-guiding fiber and generates a preferably collimated illuminating light bundle. An element for modifying the shape of the illuminating light focus, which is prealigned relative to the illuminating light bundle to be coupled out, is arranged in or on the fiber coupler.

Abstract translation: 本发明涉及一种具有将照明光聚焦到照明光焦点的目标的显微镜，并且具有传输照明光的导光纤维，并且其结构设置有光纤耦合器，该光纤耦合器将照明光从发光光束 并且产生优选准直的照明光束。 用于修改相对于待耦合出的照明光束预先对准的照明光焦点的形状的元件设置在光纤耦合器中或其上。

公开(公告)号：US20160202121A1

公开(公告)日：2016-07-14

申请号：US14916528

申请日：2014-09-03

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Vishnu Vardhan KRISHNAMACHARI

IPC: G01J3/12

CPC classification number: G01J3/1256 ,  G02B21/0032 ,  G02B21/0064 ,  G02B21/0076 ,  G02B27/1006 ,  G02F1/116

Abstract: The invention relates to a microscope having an acousto-optic apparatus (13) that, with a mechanical wave that is characterized by a preferably adjustable frequency, removes from a polychromatic and collinear detected light bundle (18) portions of illuminated light, scattered and/or reflected at a sample, having an illuminating light wavelength associated with the frequency. The microscope is notable for the fact that a crystal (30) of the acousto-optic apparatus in which the mechanical wave propagates, and the propagation direction of the mechanical wave, are oriented relative to the detected light bundle incident into the crystal in such a way that the acousto-optic apparatus deflects, with the mechanical wave, both the portion of the detected light bundle having the illuminating wavelength and a first linear polarization direction, and the portion of the detected light bundle having the illuminating wavelength and a second linear polarization direction perpendicular to the first polarization direction, and thereby removes them from the detected light bundle.

Abstract translation: 本发明涉及一种具有声光装置（13）的显微镜，该声光装置（13）利用优选可调频率表征的机械波，从多色和共线检测的光束（18）中去除被照射的光的散射和/ 或在样本处反射，具有与频率相关联的照明光波长。 显微镜是显着的，其中机械波传播的声光装置的晶体（30）和机械波的传播方向相对于入射到晶体中的检测到的光束而定向 声光设备利用机械波偏转检测到的光束的部分具有照明波长和第一线性偏振方向的方式，并且检测到的光束的部分具有照明波长和第二线偏振 垂直于第一偏振方向的方向，从而将它们从检测到的光束中去除。

公开(公告)号：US20130306398A1

公开(公告)日：2013-11-21

申请号：US13894726

申请日：2013-05-15

Applicant: Leica Microsystems CMS GmbH

Inventor： Holger BIRK ,  Volker SEYFRIED ,  Dirk-Oliver FEHRER ,  Vishnu Vardan KRISHNAMACHARI

IPC: G10K11/00

CPC classification number: G10K11/002 ,  G02B21/24 ,  G02B26/105 ,  G10K11/172 ,  G10K11/175

Abstract: An apparatus for damping sound in the optical beam path of a microscope, having an acoustic insulation housing for encapsulating a sound-emitting component, preferably a rapidly moving or oscillating beam deflection means, in particular a resonantly oscillating mirror, the housing comprising at least one optical entrance/exit opening, is characterized in that the housing, preferably the opening of the housing, is embodied and/or configured in such a way that the sound otherwise emerging from the housing is largely extinguished by destructive interference without thereby influencing the optical beam. A microscope having a corresponding apparatus is furthermore claimed.

Abstract translation: 一种用于阻尼显微镜的光束路径中的声音的装置，具有用于封装声发射部件的声学绝缘壳体，优选地是快速移动或振荡的光束偏转装置，特别是共振振动反射镜，所述壳体包括至少一个 光学入口/出口的特征在于，壳体，优选地是壳体的开口，被实施和/或构造成使得从壳体出来的声音被破坏性的干涉大大地消除，而不会影响光束 。 还要求具有相应装置的显微镜。

公开(公告)号：US20130034913A1

公开(公告)日：2013-02-07

申请号：US13648740

申请日：2012-10-10

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Werner KNEBEL

IPC: G01N21/76

CPC classification number: G02B21/0076 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/0056 ,  G03H1/0404 ,  G03H1/08 ,  G03H1/0866

Abstract: In order to investigate a specimen (30) with the aid of a microscope (20), dye particles (40, 42) in the specimen (30) are excited to fluoresce with the aid of a first illumination light beam (24). Fluorescent light proceeding from the specimen (30) is directed via an optical arrangement (34) onto an areal sensor (36), the optical arrangement (34) acting on the fluorescent light in such a way that sub-beams of the fluorescent light interfere with themselves, so that interference patterns produced as a result of the interference are imaged on a sensitive surface of the areal sensor (36) and sensed thereby. Positions of the dye particles (40, 42) within the specimen (30) are ascertained as a function of the interference patterns.

Abstract translation: 借助于显微镜（20）研究样品（30），借助于第一照明光束（24）激发样品（30）中的染料颗粒（40,42）以发荧光。 从样品（30）进行的荧光通过光学装置（34）引导到面传感器（36）上，光学装置（34）以荧光的子光束干涉 使得由于干扰而产生的干涉图案被成像在面传感器（36）的敏感表面上并被感测。 确定样品（30）内的染料颗粒（40,42）的位置作为干涉图案的函数。

公开(公告)号：US20190339201A1

公开(公告)日：2019-11-07

申请号：US16304168

申请日：2017-05-24

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Bernd WIDZGOWSKI ,  Frank HECHT

IPC: G01N21/64

Abstract: A fluorescence lifetime imaging microscopy method with time-correlated single photon counting includes periodically exciting a sample to emit fluorescence photons, with a measurement interval being defined between each two successive excitation light pulses. A value characterizing fluorescence decay behavior is determined based on detection times of detected fluorescence photons, and imaging is performed based one the value. An analog detector signal is sampled within a plurality of sampling intervals within a respective one of the measurement intervals and is converted into a sequence of discrete signal values associated with the sampling intervals. It is determined based thereon whether more than a predefined number of fluorescence photons has been detected within the respective measurement interval. If more than the predefined number of fluorescence photons has been detected, the respective measurement interval is discarded in the step of determining the value characterizing the fluorescence decay behavior.

公开(公告)号：US20160290928A1

公开(公告)日：2016-10-06

申请号：US15037451

申请日：2014-11-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI ,  Volker SEYFRIED ,  William C. HAY ,  Manuel KREMER

IPC: G01N21/65

CPC classification number: G01N21/65 ,  G01N2021/653 ,  G01N2201/06113 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/16

Abstract: A device (10) for illuminating a sample (40) is described, having: at least one pulsed laser light source (12) for repeated emission of a first laser pulse along a first light path (14) and of a second laser pulse along a second light path (16) physically separated from the first light path; a superimposition element (32) for collinear superimposition of the two laser pulses in a shared light path (34); a delay stage (26) arranged in the first or the second light path (14, 16), for delaying one of the two laser pulses relative to the other laser pulse in such a way that the two laser pulses sent along the shared light path (34) onto the sample (40) exhibit a temporal superimposition; a shared chirp unit (36) arranged in the shared light path (34), for frequency-modifying influencing both of the first laser pulse and of the second laser pulse; and at least one separate chirp unit (18) arranged in the first light path (14), for frequency-modifying influencing only of the first laser pulse. The shared chirp unit (36) and the separate chirp unit (18) are coordinated with one another in order to achieve a target state. The separate chirp unit (18) is coupled to a control system (20) by which the separate chirp unit (18) is controllable with a control parameter dependent on the wavelength of the first laser pulse in order to establish the target state.

Abstract translation: 描述了用于照亮样品（40）的装置（10），其具有：用于沿着第一光路（14）重复发射第一激光脉冲和沿着第二激光脉冲重复发射第一激光脉冲的至少一个脉冲激光光源（12） 与第一光路物理分离的第二光路（16）; 用于共享光路（34）中的两个激光脉冲的共线叠加的叠加元件（32）; 布置在第一或第二光路（14,16）中的延迟级（26），用于相对于另一激光脉冲延迟两个激光脉冲中的一个，使得两个激光脉冲沿共享光路 （34）到样品（40）上呈现时间叠加; 布置在共享光路（34）中的共享啁啾单元（36），用于对影响第一激光脉冲和第二激光脉冲两者的频率修正; 以及布置在第一光路（14）中的至少一个单独的啁啾单元（18），用于仅对第一激光脉冲影响的频率修正。 共享啁啾单元（36）和单独的啁啾单元（18）彼此协调以实现目标状态。 单独的啁啾单元（18）被耦合到控制系统（20），通过该控制系统（20），单独的啁啾单元（18）可以通过取决于第一激光脉冲的波长的控制参数来控制，以便建立目标状态。

公开(公告)号：US20160209270A1

公开(公告)日：2016-07-21

申请号：US14916502

申请日：2014-09-03

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Vishnu Vardhan KRISHNAMACHARI

IPC: G01J3/12 ,  G02B21/00 ,  G02B27/28

Abstract: The invention relates to a beam combiner for a microscope, in particular a scanning microscope, which receives at least a first illuminating light bundle and a second illuminating light bundle and combines them into a collinear output light bundle, the first illuminating light bundle and the second illuminating light bundle having the same illuminating light wavelength but a different polarization, in particular linear polarization. The beam combiner is embodied as an acousto-optic beam combiner and is constructed and operated in such a way that by interaction with at least one mechanical wave, both the first illuminating light bundle and the second illuminating light bundle are diffracted and are thereby directed into a common optical axis.

Abstract translation: 本发明涉及一种用于显微镜的光束组合器，特别是扫描显微镜，其接收至少第一照明光束和第二照明光束，并将它们组合成共线输出光束，第一照明光束和第二照明光束 照明光束具有相同的照明光波长但具有不同的偏振，特别是线偏振。 光束组合器被实现为声光束组合器，并且被构造和操作，使得通过与至少一个机械波的相互作用，第一照明光束和第二照明光束都被衍射，从而被引导到 共同的光轴。

公开(公告)号：US20160181459A1

公开(公告)日：2016-06-23

申请号：US14973786

申请日：2015-12-18

Applicant: Leica Microsystems CMS GmbH

Inventor： Holger BIRK ,  Volker SEYFRIED

IPC: H01L31/107 ,  H01L31/028

CPC classification number: H01L31/02027 ,  H01L27/1443

Abstract: A method for increasing the dynamic range of a silicon photomultiplier (SiPM) in particular with regard to the detection of high radiation intensities, individual avalanche photodiodes usually being biased with a bias voltage VR beyond a breakdown voltage VBR, with the consequence of an avalanche discharge of electrons that define the output signal, is characterized in that the output signals resulting from an incident radiation intensity are adjusted by modifying the bias voltage VR, the output signals being evaluated in the integration mode.

Abstract translation: 一种用于增加硅光电倍增管（SiPM）的动态范围的方法，特别是关于高辐射强度的检测，单个雪崩光电二极管通常以超过击穿电压VBR的偏置电压VR偏压，其结果是雪崩放电 定义输出信号的电子的特征在于，通过修改偏置电压VR来调整由入射辐射强度产生的输出信号，输出信号在积分模式下被评估。