公开(公告)号：US20160223457A1

公开(公告)日：2016-08-04

申请号：US14916538

申请日：2014-09-03

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI

IPC: G01N21/21 ,  G01N21/65 ,  G02B21/00

CPC classification number: G01N21/21 ,  G01N21/65 ,  G01N2201/067 ,  G01N2201/0683 ,  G02B21/002 ,  G02B21/0032 ,  G02B21/0068 ,  G02B27/283 ,  G02B27/286

Abstract: The invention relates to a method for investigating a sample, the sample being impinged upon by illuminating light, and detected light emerging from the sample being directed to a detector, and the illuminating light being directed through an acousto-optic component with which the impingement upon the sample by illuminating light can be temporarily interrupted. The method is notable for the fact that the sample is illuminated with a first illuminating light bundle that has a first linear polarization direction, and with a second illuminating light bundle whose linear polarization direction is continuously switched over between the first linear polarization direction and a second linear polarization direction different from the first linear polarization direction, the illuminating light having the first linear polarization direction proceeding along a first light path and illuminating light having the second linear polarization direction proceeding along a second light path, and the acousto-optic component combining the light paths.

Abstract translation: 本发明涉及一种用于研究样品的方法，通过照射光照射样品，并且检测到从样品中出射的光被引导到检测器，并且照明光被引导通过声光部件，撞击在其上 可以暂时中断通过照明光的样品。 该方法值得注意的是，样品被具有第一线偏振方向的第一照明光束照射，并且第二照明光束的线偏振方向在第一线偏振方向和第二线偏振方向之间连续切换 线偏振方向与第一线偏振方向不同的是，具有第一线偏振方向的照明光沿第一光路行进，具有第二线偏振方向的照明光沿第二光路行进，声光分量合成 光路。

公开(公告)号：US20190086257A1

公开(公告)日：2019-03-21

申请号：US16081427

申请日：2017-03-01

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI ,  Patric MRAWEK ,  Bernd WIDZGOWSKI

IPC: G01J1/02 ,  G01J1/46 ,  G01J3/44

Abstract: A light/voltage converter circuit for converting intensity fluctuations of light into an alternating voltage measurement signal includes a photodiode configured to detect the light and a transformer comprising a primary coil and a secondary coil. The primary coil is connected in a series circuit to the photodiode, and the alternating voltage measurement signal is applied to the secondary coil. An electrical network is configured to block direct current and conduct alternating current. The electrical network is connected in parallel with the series circuit consisting of the primary coil of the transformer and the photodiode.

公开(公告)号：US20160363537A1

公开(公告)日：2016-12-15

申请号：US15037455

申请日：2014-11-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI ,  Volker SEYFRIED ,  William C. HAY

IPC: G01N21/65 ,  G01J3/10 ,  G01J3/44

CPC classification number: G01N21/65 ,  G01J3/0227 ,  G01J3/10 ,  G01J3/44 ,  G01N2021/655

Abstract: A Raman microscopy imaging device (100) is described, having: a first laser light source (12) for emitting a first laser beam (16) having a first wavelength along a first light path (20); a second laser light source (44) for emitting a second laser beam (18) having a second wavelength, different from the first wavelength, along a second light path (22) physically separated from the first light path (20); a beam combining element (32) for collinearly combining the two laser beams (16, 18) in one shared light path (34) directed onto a sample; a detector (38) for sensing a measured signal on the basis of the two laser beams (16, 18) interacting with the sample; and an evaluation unit (40) for evaluating the measured signal sensed by the detector (38). According to the present invention the first laser light source (12) is embodied as a pulsed source, and the second laser light source (44) as a continuous source.

Abstract translation: 描述了一种拉曼显微镜成像装置（100），其具有：用于沿第一光路（20）发射具有第一波长的第一激光束（16）的第一激光光源（12）。 第二激光源（44），沿着与第一光路（20）物理分离的第二光路（22）发射具有与第一波长不同的第二波长的第二激光束（18）。 用于在指向样本的一个共享光路（34）中共同组合两个激光束（16,18）的光束组合元件（32） 检测器（38），用于基于与样本相互作用的两个激光束（16,18）感测测量信号; 以及用于评估由检测器（38）感测的测量信号的评估单元（40）。 根据本发明，第一激光源（12）被实现为脉冲源，第二激光源（44）作为连续源。

公开(公告)号：US20160223882A1

公开(公告)日：2016-08-04

申请号：US14916520

申请日：2014-09-03

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI

IPC: G02F1/33 ,  G02F1/11 ,  G02B21/00

CPC classification number: G02F1/33 ,  G01J3/1256 ,  G02B21/0032 ,  G02B21/0064 ,  G02B21/0068 ,  G02B27/283 ,  G02F1/116

Abstract: The invention relates to an acousto-optic main beam splitter for a scanning microscope, which is embodied and intended to direct illuminating light having a preselected or preselectable illuminating light wavelength into an illumination beam path for illumination of a sample, and to direct detected light coming from a sample into a detection beam path. The acousto-optic main beam splitter is characterized in that a mechanical wave having an acoustic frequency associated with the illuminating light wavelength propagates, or multiple mechanical waves that have the same acoustic frequency associated with the illuminating light wavelength propagate, in the acousto-optic main beam splitter, both a portion of the detected light bundle having the illuminating light wavelength and a first linear polarization direction, and a portion of the detected light having the illuminating light wavelength and a second linear polarization direction perpendicular to the first linear polarization direction, being deflected out of the detected light bundle coming from a sample by interaction with the one mechanical wave or by interaction with the mechanical waves, and are thereby removed from the detected light bundle; and/or the acousto-optic main beam splitter being embodied to direct, by interaction with the one mechanical wave or by interaction with the mechanical waves, both the portion of illuminating light that has the preselected illuminating light wavelength and a first linear polarization direction, and the portion of illuminating light that has the preselected illuminating light wavelength and a second linear polarization direction that is different from, in particular perpendicular to, the first linear polarization direction, into an illumination beam path for illumination of a sample.

Abstract translation: 本发明涉及一种用于扫描显微镜的声光主分束器，其被实施并旨在将具有预选或可选择的照明光波长的照明光引导到用于照射样品的照明光束路径中，并将检测到的光引导 从样品到检测光束路径。 声光主分束器的特征在于具有与照明光波长相关联的声频的机械波传播，或者具有与照明光波长相关联的相同声频的多个机械波在声光主体中传播 检测到的光束的一部分具有照明光波长和第一线偏振方向的一部分以及具有照射光波长的检测光的一部分和与第一线偏振方向垂直的第二线偏振方向， 通过与一个机械波的相互作用或通过与机械波的相互作用而偏离来自样品的检测到的光束，从而从检测到的光束中除去; 和/或声光主分束器被实现为通过与一个机械波相互作用或通过与机械波的相互作用来引导具有预选照明光波长的照明光的一部分和第一线偏振方向， 以及将具有预选照明光波长的照明光和不同于特别是垂直于第一线偏振方向的第二线偏振方向的部分转换成用于照射样本的照明光束路径。

公开(公告)号：US20160216498A1

公开(公告)日：2016-07-28

申请号：US14916486

申请日：2014-09-03

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Vishnu Vardhan KRISHNAMACHARI ,  Arnold GISKE

IPC: G02B21/00 ,  G02F1/29 ,  G02F1/33 ,  G02B6/26 ,  G02B27/09

CPC classification number: G02B21/0032 ,  G02B6/262 ,  G02B6/32 ,  G02B21/0068 ,  G02B21/0076 ,  G02B27/09 ,  G02B27/0905 ,  G02B27/58 ,  G02F1/292 ,  G02F1/33

Abstract: The invention relates to a microscope having an objective that focuses illuminating light to an illuminating light focus, and having a light-guiding fiber which transports the illuminating light and at whose end is arranged a fiber coupler that couples the illuminating light out of the light-guiding fiber and generates a preferably collimated illuminating light bundle. An element for modifying the shape of the illuminating light focus, which is prealigned relative to the illuminating light bundle to be coupled out, is arranged in or on the fiber coupler.

Abstract translation: 本发明涉及一种具有将照明光聚焦到照明光焦点的目标的显微镜，并且具有传输照明光的导光纤维，并且其结构设置有光纤耦合器，该光纤耦合器将照明光从发光光束 并且产生优选准直的照明光束。 用于修改相对于待耦合出的照明光束预先对准的照明光焦点的形状的元件设置在光纤耦合器中或其上。

公开(公告)号：US20160202121A1

公开(公告)日：2016-07-14

申请号：US14916528

申请日：2014-09-03

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Vishnu Vardhan KRISHNAMACHARI

IPC: G01J3/12

CPC classification number: G01J3/1256 ,  G02B21/0032 ,  G02B21/0064 ,  G02B21/0076 ,  G02B27/1006 ,  G02F1/116

Abstract: The invention relates to a microscope having an acousto-optic apparatus (13) that, with a mechanical wave that is characterized by a preferably adjustable frequency, removes from a polychromatic and collinear detected light bundle (18) portions of illuminated light, scattered and/or reflected at a sample, having an illuminating light wavelength associated with the frequency. The microscope is notable for the fact that a crystal (30) of the acousto-optic apparatus in which the mechanical wave propagates, and the propagation direction of the mechanical wave, are oriented relative to the detected light bundle incident into the crystal in such a way that the acousto-optic apparatus deflects, with the mechanical wave, both the portion of the detected light bundle having the illuminating wavelength and a first linear polarization direction, and the portion of the detected light bundle having the illuminating wavelength and a second linear polarization direction perpendicular to the first polarization direction, and thereby removes them from the detected light bundle.

Abstract translation: 本发明涉及一种具有声光装置（13）的显微镜，该声光装置（13）利用优选可调频率表征的机械波，从多色和共线检测的光束（18）中去除被照射的光的散射和/ 或在样本处反射，具有与频率相关联的照明光波长。 显微镜是显着的，其中机械波传播的声光装置的晶体（30）和机械波的传播方向相对于入射到晶体中的检测到的光束而定向 声光设备利用机械波偏转检测到的光束的部分具有照明波长和第一线性偏振方向的方式，并且检测到的光束的部分具有照明波长和第二线偏振 垂直于第一偏振方向的方向，从而将它们从检测到的光束中去除。

公开(公告)号：US20190339500A1

公开(公告)日：2019-11-07

申请号：US16305902

申请日：2017-05-31

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Bernd WIDZGOWSKI ,  Vishnu Vardhan KRISHNAMACHARI ,  Lars FRIEDRICH ,  Manuel KREMER ,  Arnold GISKE

IPC: G02B21/00

Abstract: An optical arrangement has an optical beam path for illuminating a sample space with a sequence of laser light pulses generated in a laser cycle, the optical arrangement. At least one laser light source is configured to generate the sequence of laser light pulses along the optical beam path. A wavelength-selective pulse picker is situated in the optical beam path and has, in a predefined illumination clock timing synchronizable with the laser light pulses, an open state in which the pulse picker is light-transparent to at least one laser light pulse towards the sample space. The open state has at least two different transmission states which differ with regard to their respective transmission spectrums, and wherein the two transmission states are switchable on and/or off independently of one another.

公开(公告)号：US20160290928A1

公开(公告)日：2016-10-06

申请号：US15037451

申请日：2014-11-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI ,  Volker SEYFRIED ,  William C. HAY ,  Manuel KREMER

IPC: G01N21/65

CPC classification number: G01N21/65 ,  G01N2021/653 ,  G01N2201/06113 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/16

Abstract: A device (10) for illuminating a sample (40) is described, having: at least one pulsed laser light source (12) for repeated emission of a first laser pulse along a first light path (14) and of a second laser pulse along a second light path (16) physically separated from the first light path; a superimposition element (32) for collinear superimposition of the two laser pulses in a shared light path (34); a delay stage (26) arranged in the first or the second light path (14, 16), for delaying one of the two laser pulses relative to the other laser pulse in such a way that the two laser pulses sent along the shared light path (34) onto the sample (40) exhibit a temporal superimposition; a shared chirp unit (36) arranged in the shared light path (34), for frequency-modifying influencing both of the first laser pulse and of the second laser pulse; and at least one separate chirp unit (18) arranged in the first light path (14), for frequency-modifying influencing only of the first laser pulse. The shared chirp unit (36) and the separate chirp unit (18) are coordinated with one another in order to achieve a target state. The separate chirp unit (18) is coupled to a control system (20) by which the separate chirp unit (18) is controllable with a control parameter dependent on the wavelength of the first laser pulse in order to establish the target state.

Abstract translation: 描述了用于照亮样品（40）的装置（10），其具有：用于沿着第一光路（14）重复发射第一激光脉冲和沿着第二激光脉冲重复发射第一激光脉冲的至少一个脉冲激光光源（12） 与第一光路物理分离的第二光路（16）; 用于共享光路（34）中的两个激光脉冲的共线叠加的叠加元件（32）; 布置在第一或第二光路（14,16）中的延迟级（26），用于相对于另一激光脉冲延迟两个激光脉冲中的一个，使得两个激光脉冲沿共享光路 （34）到样品（40）上呈现时间叠加; 布置在共享光路（34）中的共享啁啾单元（36），用于对影响第一激光脉冲和第二激光脉冲两者的频率修正; 以及布置在第一光路（14）中的至少一个单独的啁啾单元（18），用于仅对第一激光脉冲影响的频率修正。 共享啁啾单元（36）和单独的啁啾单元（18）彼此协调以实现目标状态。 单独的啁啾单元（18）被耦合到控制系统（20），通过该控制系统（20），单独的啁啾单元（18）可以通过取决于第一激光脉冲的波长的控制参数来控制，以便建立目标状态。

公开(公告)号：US20160209270A1

公开(公告)日：2016-07-21

申请号：US14916502

申请日：2014-09-03

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Vishnu Vardhan KRISHNAMACHARI

IPC: G01J3/12 ,  G02B21/00 ,  G02B27/28

Abstract: The invention relates to a beam combiner for a microscope, in particular a scanning microscope, which receives at least a first illuminating light bundle and a second illuminating light bundle and combines them into a collinear output light bundle, the first illuminating light bundle and the second illuminating light bundle having the same illuminating light wavelength but a different polarization, in particular linear polarization. The beam combiner is embodied as an acousto-optic beam combiner and is constructed and operated in such a way that by interaction with at least one mechanical wave, both the first illuminating light bundle and the second illuminating light bundle are diffracted and are thereby directed into a common optical axis.

Abstract translation: 本发明涉及一种用于显微镜的光束组合器，特别是扫描显微镜，其接收至少第一照明光束和第二照明光束，并将它们组合成共线输出光束，第一照明光束和第二照明光束 照明光束具有相同的照明光波长但具有不同的偏振，特别是线偏振。 光束组合器被实现为声光束组合器，并且被构造和操作，使得通过与至少一个机械波的相互作用，第一照明光束和第二照明光束都被衍射，从而被引导到 共同的光轴。