公开(公告)号：US20190339500A1

公开(公告)日：2019-11-07

申请号：US16305902

申请日：2017-05-31

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Bernd WIDZGOWSKI ,  Vishnu Vardhan KRISHNAMACHARI ,  Lars FRIEDRICH ,  Manuel KREMER ,  Arnold GISKE

IPC: G02B21/00

Abstract: An optical arrangement has an optical beam path for illuminating a sample space with a sequence of laser light pulses generated in a laser cycle, the optical arrangement. At least one laser light source is configured to generate the sequence of laser light pulses along the optical beam path. A wavelength-selective pulse picker is situated in the optical beam path and has, in a predefined illumination clock timing synchronizable with the laser light pulses, an open state in which the pulse picker is light-transparent to at least one laser light pulse towards the sample space. The open state has at least two different transmission states which differ with regard to their respective transmission spectrums, and wherein the two transmission states are switchable on and/or off independently of one another.

公开(公告)号：US20250084458A1

公开(公告)日：2025-03-13

申请号：US18284550

申请日：2022-03-31

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Soeren ALSHEIMER ,  Arnold GISKE

IPC: C12Q1/6818 ,  G01N33/542

Abstract: A capture construct for capturing a plurality of analytes of a biological sample includes a nanostructure backbone, at least a first orientation indicator and a second orientation indicator, and at least a first plurality of capture regions on the nanostructure backbone. Each capture region includes at least one affinity capture reagent configured to capture one of the plurality of analytes.

公开(公告)号：US20160216498A1

公开(公告)日：2016-07-28

申请号：US14916486

申请日：2014-09-03

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Vishnu Vardhan KRISHNAMACHARI ,  Arnold GISKE

IPC: G02B21/00 ,  G02F1/29 ,  G02F1/33 ,  G02B6/26 ,  G02B27/09

CPC classification number: G02B21/0032 ,  G02B6/262 ,  G02B6/32 ,  G02B21/0068 ,  G02B21/0076 ,  G02B27/09 ,  G02B27/0905 ,  G02B27/58 ,  G02F1/292 ,  G02F1/33

Abstract: The invention relates to a microscope having an objective that focuses illuminating light to an illuminating light focus, and having a light-guiding fiber which transports the illuminating light and at whose end is arranged a fiber coupler that couples the illuminating light out of the light-guiding fiber and generates a preferably collimated illuminating light bundle. An element for modifying the shape of the illuminating light focus, which is prealigned relative to the illuminating light bundle to be coupled out, is arranged in or on the fiber coupler.

Abstract translation: 本发明涉及一种具有将照明光聚焦到照明光焦点的目标的显微镜，并且具有传输照明光的导光纤维，并且其结构设置有光纤耦合器，该光纤耦合器将照明光从发光光束 并且产生优选准直的照明光束。 用于修改相对于待耦合出的照明光束预先对准的照明光焦点的形状的元件设置在光纤耦合器中或其上。

公开(公告)号：US20230008453A1

公开(公告)日：2023-01-12

申请号：US17779592

申请日：2020-11-18

Applicant: Leica Microsystems CMS GmbH

Inventor： Arnold GISKE ,  Jonas FOELLING

IPC: G02B21/00 ,  G01N21/64

Abstract: A laser scanning microscope includes a light source configured to emit an illumination light beam. The illumination light beam has a transverse light intensity profile comprising an intensity minimum. The laser scanning microscope further includes a scanning device configured to scan the illumination light beam along a closed trajectory in a target area of a specimen, and a detector configured to detect fluorescence light emitted by a fluorophore within the target area of the specimen. The fluorophore is excited by the illumination light beam. The laser scanning microscope further includes a processor configured to determine an intensity distribution of the fluorescence light as a function of time and to determine a position of the fluorophore within the target area based on the intensity distribution of the fluorescence light.