公开(公告)号：US20160363537A1

公开(公告)日：2016-12-15

申请号：US15037455

申请日：2014-11-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI ,  Volker SEYFRIED ,  William C. HAY

IPC: G01N21/65 ,  G01J3/10 ,  G01J3/44

CPC classification number: G01N21/65 ,  G01J3/0227 ,  G01J3/10 ,  G01J3/44 ,  G01N2021/655

Abstract: A Raman microscopy imaging device (100) is described, having: a first laser light source (12) for emitting a first laser beam (16) having a first wavelength along a first light path (20); a second laser light source (44) for emitting a second laser beam (18) having a second wavelength, different from the first wavelength, along a second light path (22) physically separated from the first light path (20); a beam combining element (32) for collinearly combining the two laser beams (16, 18) in one shared light path (34) directed onto a sample; a detector (38) for sensing a measured signal on the basis of the two laser beams (16, 18) interacting with the sample; and an evaluation unit (40) for evaluating the measured signal sensed by the detector (38). According to the present invention the first laser light source (12) is embodied as a pulsed source, and the second laser light source (44) as a continuous source.

Abstract translation: 描述了一种拉曼显微镜成像装置（100），其具有：用于沿第一光路（20）发射具有第一波长的第一激光束（16）的第一激光光源（12）。 第二激光源（44），沿着与第一光路（20）物理分离的第二光路（22）发射具有与第一波长不同的第二波长的第二激光束（18）。 用于在指向样本的一个共享光路（34）中共同组合两个激光束（16,18）的光束组合元件（32） 检测器（38），用于基于与样本相互作用的两个激光束（16,18）感测测量信号; 以及用于评估由检测器（38）感测的测量信号的评估单元（40）。 根据本发明，第一激光源（12）被实现为脉冲源，第二激光源（44）作为连续源。

公开(公告)号：US20160290928A1

公开(公告)日：2016-10-06

申请号：US15037451

申请日：2014-11-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI ,  Volker SEYFRIED ,  William C. HAY ,  Manuel KREMER

IPC: G01N21/65

CPC classification number: G01N21/65 ,  G01N2021/653 ,  G01N2201/06113 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/16

Abstract: A device (10) for illuminating a sample (40) is described, having: at least one pulsed laser light source (12) for repeated emission of a first laser pulse along a first light path (14) and of a second laser pulse along a second light path (16) physically separated from the first light path; a superimposition element (32) for collinear superimposition of the two laser pulses in a shared light path (34); a delay stage (26) arranged in the first or the second light path (14, 16), for delaying one of the two laser pulses relative to the other laser pulse in such a way that the two laser pulses sent along the shared light path (34) onto the sample (40) exhibit a temporal superimposition; a shared chirp unit (36) arranged in the shared light path (34), for frequency-modifying influencing both of the first laser pulse and of the second laser pulse; and at least one separate chirp unit (18) arranged in the first light path (14), for frequency-modifying influencing only of the first laser pulse. The shared chirp unit (36) and the separate chirp unit (18) are coordinated with one another in order to achieve a target state. The separate chirp unit (18) is coupled to a control system (20) by which the separate chirp unit (18) is controllable with a control parameter dependent on the wavelength of the first laser pulse in order to establish the target state.

Abstract translation: 描述了用于照亮样品（40）的装置（10），其具有：用于沿着第一光路（14）重复发射第一激光脉冲和沿着第二激光脉冲重复发射第一激光脉冲的至少一个脉冲激光光源（12） 与第一光路物理分离的第二光路（16）; 用于共享光路（34）中的两个激光脉冲的共线叠加的叠加元件（32）; 布置在第一或第二光路（14,16）中的延迟级（26），用于相对于另一激光脉冲延迟两个激光脉冲中的一个，使得两个激光脉冲沿共享光路 （34）到样品（40）上呈现时间叠加; 布置在共享光路（34）中的共享啁啾单元（36），用于对影响第一激光脉冲和第二激光脉冲两者的频率修正; 以及布置在第一光路（14）中的至少一个单独的啁啾单元（18），用于仅对第一激光脉冲影响的频率修正。 共享啁啾单元（36）和单独的啁啾单元（18）彼此协调以实现目标状态。 单独的啁啾单元（18）被耦合到控制系统（20），通过该控制系统（20），单独的啁啾单元（18）可以通过取决于第一激光脉冲的波长的控制参数来控制，以便建立目标状态。