-
公开(公告)号：US11454781B2
公开(公告)日：2022-09-27
申请号：US17085962
申请日：2020-10-30
发明人： Allen Olson , Kiran Saligrama , Yunlu Zou , Peyman Najmabadi
摘要： A digital scanning apparatus is provided that includes imaging and focusing sensors and a processor to analyze the image data captured by the imaging and focusing sensors and adjust the focus of the scanning apparatus in real time during a scanning operation. The individual pixels of the imaging sensor are all in the same image plane with respect to the optical path of the digital scanning apparatus. The individual pixels of the focusing sensor are each in a different image plane with respect to the optical path, and one pixel of the focusing sensor is on the same image plane as the image sensor. The processor analyzes image data from the imaging sensor and the focusing sensor and determines a distance and direction to adjust the relative position of an objective lens and a stage of the digital scanning apparatus to achieve optimal focus during the scanning operation.
-
公开(公告)号：US10823936B2
公开(公告)日：2020-11-03
申请号：US16601355
申请日：2019-10-14
发明人： Allen Olson , Kiran Saligrama , Yunlu Zou , Peyman Najmabadi
摘要： A digital scanning apparatus is provided that includes imaging and focusing sensors and a processor to analyze the image data captured by the imaging and focusing sensors and adjust the focus of the scanning apparatus in real time during a scanning operation. The individual pixels of the imaging sensor are all in the same image plane with respect to the optical path of the digital scanning apparatus. The individual pixels of the focusing sensor are each in a different image plane with respect to the optical path, and one pixel of the focusing sensor is on the same image plane as the image sensor. The processor analyzes image data from the imaging sensor and the focusing sensor and determines a distance and direction to adjust the relative position of an objective lens and a stage of the digital scanning apparatus to achieve optimal focus during the scanning operation.
-
公开(公告)号：US10591710B2
公开(公告)日：2020-03-17
申请号：US15763061
申请日：2016-09-23
发明人： Yunlu Zou , Allen Olson , Kiran Saligrama , Ruby Chen , Peyman Najmabadi , Greg Crandall
摘要： System for acquiring a digital image of a sample on a microscope slide. In an embodiment, the system comprises a stage configured to support a sample, an objective lens having a single optical axis that is orthogonal to the stage, an imaging sensor, and a focusing sensor. The system further comprises at least one beam splitter optically coupled to the objective lens and configured to receive a field of view corresponding to the optical axis of the objective lens, and simultaneously provide at least a first portion of the field of view to the imaging sensor and at least a second portion of the field of view to the focusing sensor. The focusing sensor may simultaneously acquire image(s) at a plurality of different focal distances and/or simultaneously acquire a pair of mirrored images, each comprising pixels acquired at a plurality of different focal distances.
-
公开(公告)号：US10585273B2
公开(公告)日：2020-03-10
申请号：US16146991
申请日：2018-09-28
发明人： Leng-Chun Chen , Yunlu Zou , Allen Olson , Peyman Najmabadi , Greg Crandall , Nicholas Newberg , Aaron Stearrett
摘要： Two-pass capture of a macro image. In an embodiment, a scanning apparatus comprises a stage, a high-resolution camera, and a lens that provides a field of view, substantially equal in width to a slide width, to the high-resolution camera. The apparatus also comprises a first illumination system for transmission-mode illumination, and a second illumination system for reflection-mode illumination. Processor(s) move the stage in a first direction to capture a first macro image of a specimen during a single pass while the field of view is illuminated by the first illumination system, and move the stage in a second direction to capture a second macro image of the specimen during a single pass while the field of view is illuminated by the second illumination system. The processor(s) identify artifacts in the second macro image, and, based on those artifacts, correct the first macro image to generate a modified first macro image.
-
公开(公告)号：US11863867B2
公开(公告)日：2024-01-02
申请号：US17309716
申请日：2020-08-05
发明人： Allen H. Olson , Yunlu Zou , Bharat Annaldas , Leng-Chun Chen
CPC分类号： H04N23/67 , G02B21/002 , G02B21/244 , G02B21/367 , H04N3/06 , H04N23/90 , H04N23/56
摘要： Real-time focusing in a slide-scanning system. In an embodiment, focus points are added to an initialized focus map while acquiring a plurality of image stripes of a sample on a glass slide. For each image stripe, a plurality of frames, collectively representing the image stripe, may be acquired using both an imaging line-scan camera and a tilted focusing line-scan camera. Focus points, representing positions of best focus for trusted frames, are added to the focus map. Outlying focus points are removed from the focus map. In some cases, one or more image stripes may be reacquired. Finally, the image stripes are assembled into a composite image of the sample.
-
公开(公告)号：US20220163779A1
公开(公告)日：2022-05-26
申请号：US17650221
申请日：2022-02-07
发明人： Yunlu Zou , Gregory Crandall , Allen Olson
摘要： Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.
-
公开(公告)号：US10895726B2
公开(公告)日：2021-01-19
申请号：US16705012
申请日：2019-12-05
发明人： Yunlu Zou , Peyman Najmabadi
摘要： Apparatus and methods for scanning a 2D or 3D image of a specimen without relative Z-axis motion between the specimen and the objective lens. In an embodiment, the apparatus includes a tilted camera having individual lines of pixels. Each line of pixels can be separately processed and is at a different image plane with respect to the stage. The depth of field of each line of pixels abuts, slightly overlaps, or is slightly spaced apart from the adjacent lines of pixels in the tilted camera. The angle of the tilt determines the relationship (abut, overlapping, or spaced) of the adjacent lines of pixels. The individual image lines produced by each line of pixels can be combined into a 3D volume image of a sample. Also, the highest-contrast line at each X-Y location can be combined into an in-focus 2D image of the sample.
-
公开(公告)号：US10852523B2
公开(公告)日：2020-12-01
申请号：US16674742
申请日：2019-11-05
发明人： Leng-Chun Chen , Allen Olson , Yunlu Zou , Peyman Najmabadi , Greg Crandall
摘要： Real-time autofocus. In an embodiment, a scanning apparatus includes an imaging sensor, a focusing sensor, an objective lens, and processor(s) configured to analyze image data captured by the imaging and focusing sensors, and move the objective lens. Real-time autofocus during scanning of a sample is achieved by determining a true-Z value for the objective lens for a point on a sample and for each of a plurality of regions on the sample. The true-Z values and/or surfaces calculated therefrom are used to determine a predicted-Z value for an unscanned region of the sample. The objective lens is adjusted to the predicted-Z value at the beginning of the unscanned region. After scanning the region, a true-Z value is determined for the region and compared to the predicted-Z value. A rescan of the region is initiated if the comparison exceeds a predetermined threshold.
-
公开(公告)号：US20220373777A1
公开(公告)日：2022-11-24
申请号：US17753775
申请日：2020-10-12
发明人： Yunlu Zou
摘要： Subpixel line scanning. A slide scanning device comprises a plurality of line sensors (112a, 112b, 112c), each comprising a plurality of pixel sensors. Each line sensor is offset from an adjacent line sensor by a fraction of a length of each pixel sensor, and generates a line image of the same field of view at its respective offset. For each of a plurality of positions on a sample, a processor combines the line images of the same field of view, generated by the plurality of line sensors at their respective offsets, to produce a plurality of subpixels for each of at least a subset of pixels within the line images of the same field of view, and generates an up-sampled line image of the position comprising the plurality of subpixels. Then, the processor combines the up-sampled line images of each of the plurality of positions on the sample into an image.
-
公开(公告)号：US20220155578A1
公开(公告)日：2022-05-19
申请号：US17309733
申请日：2020-08-06
发明人： Yunlu Zou
摘要： Physical calibration slide comprising a plurality of etched features. The etched features may comprise two or more of a slanted Ronchi ruling feature comprising at least one set of parallel lines that are slanted with respect to a long and short axis of the slide, a straight Ronchi ruling feature comprising at least one set of parallel lines that are parallel to either the long or short axis, a star target feature comprising a circle with a plurality of wedge pairs, a crosshair feature comprising at least one crosshair, a clear area, a letter-O feature comprising a circle, a resolution target feature comprising one or more resolution targets, a bullseye feature comprising a two-dimensional array of bullseyes, a triangle feature comprising at least one isosceles triangle, or a symmetric corers feature comprising two symmetric sets of geometric shapes arranged in an L-shape.
-
-
-
-
-
-
-
-
-
搜索结果
28 条记录 (耗时 0.019 秒)
