公开(公告)号：US20100278696A1

公开(公告)日：2010-11-04

申请号：US12237498

申请日：2008-09-25

Applicant: Koji KURUMA ,  Koichi Minami

Inventor： Koji KURUMA ,  Koichi Minami

IPC: G01N33/543 ,  G01N21/17 ,  G01N21/41

CPC classification number: G01N33/54373 ,  C12Q1/002 ,  G01N33/54393

Abstract: It is an object of the present invention to provide a biosensor having excellent preservation stability. The present invention provides a biosensor wherein a first hydrophilic polymer layer is immobilized on a self-assembled membrane applied onto the surface of a metal substrate, a second hydrophilic polymer layer is applied onto the first hydrophilic polymer layer, and the total film thickness of the first hydrophilic polymer layer and the second hydrophilic polymer layer in a dry state is between 20 nm and 100 nm.

Abstract translation: 本发明的目的是提供具有优异的保存稳定性的生物传感器。 本发明提供了一种生物传感器，其中将第一亲水性聚合物层固定在施加到金属基材表面上的自组装膜上，将第二亲水性聚合物层施加到第一亲水性聚合物层上， 处于干燥状态的第一亲水性聚合物层和第二亲水性聚合物层在20nm和100nm之间。

公开(公告)号：US20110237462A1

公开(公告)日：2011-09-29

申请号：US13132834

申请日：2009-12-04

Applicant: Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

Inventor： Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

IPC: C40B40/10 ,  C40B50/14

CPC classification number: G01N33/54353

Abstract: An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein antibody fragments of each set include at least two types of separate antibody fragments, the at least two types of separate antibody fragments include at least one type of labeled antibody fragment having a labeled site labeled with a luminescent substance and at least one type of acceptor antibody fragment having an acceptor site for accepting emission from the labeled antibody fragment, and the at least one type of labeled antibody fragment and the at least one type of acceptor antibody fragment are capable of cooperatively recognizing one type of antigen in combination and are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.

Abstract translation: 抗体片段固定的底物包括底物和至少一组抗体片段，其中每组的抗体片段包括至少两种类型的分离的抗体片段，所述至少两种类型的分离的抗体片段包括至少一种类型的 具有用发光物质标记的标记位点的标记抗体片段和至少一种具有用于接受来自标记抗体片段的发射的受体位点的受体抗体片段，以及至少一种类型的标记抗体片段和至少一种类型 的受体抗体片段能够组合地协同识别一种类型的抗原，并以允许一组中的每个抗体片段结合相同抗原的位置关系独立地固定在底物上。

公开(公告)号：US20090081371A1

公开(公告)日：2009-03-26

申请号：US12212942

申请日：2008-09-18

Applicant: Koichi Minami ,  Morihito Ikeda ,  Yohsuke Takeuchi

Inventor： Koichi Minami ,  Morihito Ikeda ,  Yohsuke Takeuchi

IPC: B05D3/00 ,  C12M1/34

CPC classification number: G01N33/54353 ,  C07K17/06 ,  C07K17/14 ,  G01N33/54386 ,  G01N33/54393

Abstract: A substrate includes: a base material; and chelators which are three dimensionally covalently bound to the base material at a density of 7.8×1015/mm3 or greater and 4.5×1017/mm3 or less, or two dimensionally covalently bound to the base material at a planar density of 2.0×1011/mm2 or greater and 1.0×1013/mm2 or less. Metal ions are coordinately bound to the chelators, and a bioactive substance having histidine tags are coordinately bound to the metal ions. A blocking agent having ligand sets are coordinately bound to the metal ions to which the bioactive substance is not coordinately bound.

Abstract translation: 基板包括：基材; 和以7.8×10 15 / mm 3以上且4.5×10 17 / mm 3以下的密度三维共价结合于基材的螯合剂，或者以2.0×10 11 / mm 2以上的平面密度二维共价结合到基材上 和1.0×10 13 / mm 2以下。 金属离子与螯合剂配位结合，具有组氨酸标签的生物活性物质与金属离子配位结合。 具有配体组的封闭剂与生物活性物质不配位结合的金属离子配位结合。

公开(公告)号：US20090062515A1

公开(公告)日：2009-03-05

申请号：US12201594

申请日：2008-08-29

Applicant: Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

Inventor： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

IPC: C07K1/00

CPC classification number: G01N33/54353 ,  C07K1/22 ,  G01N33/54386 ,  G01N33/54393

Abstract: A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

Abstract translation: 净化生物活性物质的方法包括以下步骤：使具有组氨酸单元的生物活性物质与底物接触的介质，物理上附着于基材表面的配体和Cu（II）或Fe（II） 与配体共价键合的金属离子; 使生物活性物质通过组氨酸单元与金属离子共价键合; 并用介质的体积为60倍量的1nmol / L至10mmol / L的咪唑衍生物溶液洗涤培养基。 在金属离子为Cu（II）的情况下，通过组氨酸单元与Cu（II）共价键合的生物活性物质通过10mmol / L至1mol / L咪唑衍生物溶液中的一种回收， 0.5 mmol / L至5 mol / L EDTA溶液。

公开(公告)号：US4382597A

公开(公告)日：1983-05-10

申请号：US187567

申请日：1980-09-15

Applicant: Koichi Minami

Inventor： Koichi Minami

IPC: A63F7/02 ,  A63F7/06 ,  A63F7/00

CPC classification number: A63F7/2409 ,  A63F7/025 ,  A63F7/066 ,  A63F2250/04 ,  A63F2250/0407

Abstract: A pinball game is contained within a housing which includes a liquid filled chamber. At least one ball is located within the liquid within the chamber. The ball has a specific gravity heavier than the liquid such that it tends to sink within the liquid but it is light enough to be conveyed upwardly by currents within the liquid. A flipper is located within the chamber and is capable of interacting with the ball to propel it within the confines of the chamber. The flipper is controlled by an actuator member located on the housing and operatively connected to the flipper to move the flipper. At least one goal receptacle is positioned within the chamber and the ball is capable of being located or deposited in the goal receptacle. It is the object to deposit the ball in the goal receptacle by manipulating the ball with the flippers and/or currents of liquid.

Abstract translation: 弹珠游戏包含在包括充满液体的腔室的壳体内。 至少一个球位于腔室内的液体内。 球具有比液体更重的比重，使得其倾向于在液体内沉降，但是其足够轻以通过液体内的电流被向上输送。 导板位于腔室内，能够与球体相互作用，以将其推进腔室的范围内。 导板由位于壳体上的致动器构件控制，并且可操作地连接到导板以移动导板。 至少一个目标容器定位在腔室内，并且球能够定位或沉积在球门座中。 目标是通过用脚蹼和/或液体流动操纵球，将球沉积在球门座中。

公开(公告)号：US09487592B2

公开(公告)日：2016-11-08

申请号：US13125478

申请日：2009-10-23

Applicant: Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

Inventor： Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

IPC: C07K16/18 ,  C07K16/40

CPC classification number: C07K16/40 ,  C07K16/18 ,  C07K2317/56 ,  Y10T428/269

Abstract: An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein the antibody fragments of each set includes at least two types of separate antibody fragments that are capable of recognizing one type of antigen and that are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.

Abstract translation: 抗体片段固定底物包括底物和至少一组抗体片段，其中每组的抗体片段包含能够识别一种类型的抗原并且独立地固定在其上的至少两种类型的分离的抗体片段 位置关系的底物允许一组中的每个抗体片段与相同的抗原结合。

公开(公告)号：US20100290954A1

公开(公告)日：2010-11-18

申请号：US12744311

申请日：2008-11-21

Applicant: Koichi Minami ,  Hirohiko Tsuzuki

Inventor： Koichi Minami ,  Hirohiko Tsuzuki

IPC: G01N21/64 ,  G01N33/543

CPC classification number: G01N33/542 ,  G01N21/6428

Abstract: A first reactant, which is provided with a reaction site for specific binding with an analyte, and a fluorescent label site, and a second reactant, which is provided with a reaction site for specific binding with the analyte, and a fluorescence recognition site for recognizing fluorescence produced by the fluorescent label site of the first reactant, are respectively fixed onto a support such that the first reactant and the second reactant have a positional relationship adapted for the binding with the analyte.

Abstract translation: 提供有用于与分析物特异性结合的反应位点的第一反应物和荧光标记位点，以及第二反应物，其具有用于与分析物特异性结合的反应位点和用于识别的荧光识别位点 由第一反应物的荧光标记位点产生的荧光分别固定在载体上，使得第一反应物和第二反应物具有适于与分析物结合的位置关系。

公开(公告)号：US4480834A

公开(公告)日：1984-11-06

申请号：US433031

申请日：1982-10-06

Applicant: Koichi Minami

Inventor： Koichi Minami

IPC: A63F7/06 ,  A63F7/20 ,  A63H11/06

CPC classification number: A63F7/0612 ,  A63H11/06 ,  A63F7/249

Abstract: A toy has a base with a surface thereon. A portion of the surface is movable with respect to the remainder of the surface. A target is mounted on a pedestal above the surface. A self-propelled object is associated with the surface. The object is of the type having a body section and a pedestal section, with a motor located in the body section which is capable of moving the pedestal section with respect to the body section. The object further includes a hand-shaped member which is capable of locking with a post which projects upwardly from the surface of the base near the movable portion of the surface. A moving member is associated with the movable portion of the surface for moving that portion. The moving member is utilized to move the movable portion of the surface such that when the object is located on that portion as the portion moves then the object is also moved.

Abstract translation: 玩具具有在其上的表面的基座。 表面的一部分相对于表面的其余部分是可移动的。 目标安装在表面上方的基座上。 自走物体与表面相关联。 该物体是具有主体部分和基座部分的类型，其中电动机位于主体部分中，其能够相对于主体部分移动基座部分。 该目的还包括手形构件，该手形构件能够利用从表面的可移动部分附近的基部表面向上突出的柱来锁定。 移动构件与用于移动该部分的表面的可移动部分相关联。 移动构件用于移动表面的可移动部分，使得当物体位于该部分上时，随着部分移动，物体也被移动。

公开(公告)号：US4468033A

公开(公告)日：1984-08-28

申请号：US417330

申请日：1982-09-13

Applicant: Koichi Minami ,  Tohru Nishimiya

Inventor： Koichi Minami ,  Tohru Nishimiya

IPC: A63F7/04 ,  A63F9/00 ,  A63F9/08

CPC classification number: A63F9/0823 ,  A63F9/0819

Abstract: A toy game of skill has a housing and a plurality of members rotatably mounted on the housing such that they are in operative association with one another. One of the members is rotated, and in so rotating that member, the rotation is communicated to the rest of the members to simultaneously rotate them. Located on the members are a plurality of object repositories. A plurality of objects, at least one less than the number of object repositories, are positioned within the object repositories. As the members move with respect to one another, the objects are carried on the object repositories. The objects can be transferred from one repository to an empty repository when two of the members are in a position aligning their respective object repositories with one another. However, the objects are retained in any one object repository whenever the members are in a position with respect to one another such that the object repositories are not aligned.

Abstract translation: 本领域的玩具游戏具有壳体和可旋转地安装在壳体上的多个构件，使得它们彼此可操作地相关联。 其中一个构件被旋转，并且在旋转该构件的同时，旋转与构件的其余部分连通以同时旋转它们。 位于成员上的是多个对象库。 多个对象（至少少于对象存储库的数量）位于对象存储库内。 当成员相对于彼此移动时，对象被承载在对象存储库上。 当两个成员处于将其各自的对象存储库彼此对齐的位置时，可以将这些对象从一个存储库传输到一个空的存储库。 但是，只要成员处于相对于彼此的位置，对象库就不对齐，则对象将保留在任何一个对象存储库中。

公开(公告)号：US4363483A

公开(公告)日：1982-12-14

申请号：US171661

申请日：1980-07-24

Applicant: Koichi Minami

Inventor： Koichi Minami

IPC: A63F7/02 ,  A63F7/04 ,  A63F7/06 ,  A63F9/00

CPC classification number: A63F7/2409 ,  A63F7/045 ,  A63F7/06 ,  A63F7/066

Abstract: A game of the type wherein a transparent housing is filled with a liquid and a movable member is located in the liquid can be made competitive by utilizing a first and second circulating member each capable of ejecting a stream of liquid from a nozzle to move the movable member. The game is improved by incorporating within the interior of a housing two separate pivoting members. Each of the pivoting members is located in association with one of the nozzles and moves in response to impingement of liquid ejected from the nozzles. The ejected liquid impinges upon the surface of the pivoting members to both pivot the members and deflect the stream of liquid. The degree of pivoting and the amount of deflection are dependent on the force of ejection of the stream of liquid. The movable member can be moved within the body of the liquid either by direct contact with one of the pivoting members, by contact with currents in the liquid which are associated with movement of the pivoting members or directed by the ejected streams of liquid.

Abstract translation: 通过利用第一和第二循环构件，能够使透明壳体填充有液体并且可移动构件位于液体中的类型的游戏具有竞争性，所述第一和第二循环构件能够从喷嘴喷射液体流以移动可移动 会员。 通过在壳体的内部并入两个单独的枢转构件来改善游戏。 每个枢转构件与其中一个喷嘴相关联并且响应于从喷嘴喷射的液体的冲击而移动。 喷射的液体撞击在枢转构件的表面上，以使构件枢转并使液流偏转。 旋转的程度和偏转量取决于液体流的喷射力。 可移动构件可以通过与液体中的一部分直接接触而与液体中的一个直接接触，该液体中的电流与枢转构件的运动相关联或被排出的液体流引导。