公开(公告)号：US20180051291A1

公开(公告)日：2018-02-22

申请号：US15556458

申请日：2016-03-17

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Sang Yup Lee ,  Tong Un Chae ,  Chan Woo Song

IPC: C12N15/52 ,  C12N1/20 ,  C12N15/01 ,  C12N15/63

CPC classification number: C12N15/52 ,  C12N1/20 ,  C12N9/88 ,  C12N15/01 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P13/001 ,  C12P13/005 ,  C12P13/04 ,  C12P13/06 ,  C12R1/00

Abstract: The present invention relates to a mutant organism having the ability to produce aspartic acid derivatives, wherein a gene encoding the glyoxylate shunt regulator and a gene encoding fumarase are deleted and a gene encoding aspartase is overexpressed compared to that in a wild-type strain, and to a method for producing L-aspartic acid derivatives using the same. According to the present invention, various aspartic acid derivatives, including L-alanine, 3-aminopropionic acid, threonine, 1,3-diaminopropane, lysine, methionine, 3-hydroxypropionic acid, cadaverine, 5-aminovaleric acid, etc., can be produced by biological methods.

公开(公告)号：US09932596B2

公开(公告)日：2018-04-03

申请号：US15299928

申请日：2016-10-21

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Sang Yup Lee ,  Tong Un Chae

IPC: C12P13/00 ,  C12N1/16 ,  C12N1/20 ,  C12N15/52 ,  C12N9/10 ,  C12N9/88

CPC classification number: C12N15/52 ,  C12N1/16 ,  C12N1/20 ,  C12N9/1096 ,  C12N9/88 ,  C12P13/001 ,  C12Y206/01 ,  C12Y401/01086

Abstract: The present disclosure relates to a recombinant microorganism producing 1,3-diaminopropane, and a method for producing 1,3-diaminopropane using the same, and specifically, to a recombinant microorganism producing 1,3-diaminopropane into which genes encoding an enzyme involved in a metabolic pathway of 1,3-diaminopropane, dat and ddc, are introduced, and a method for producing 1,3-diaminopropane using the same.When the recombinant microorganism producing the 1,3-diaminopropane according to the present disclosure is used, 1,3-diaminopropane may be mass-produced to be industrially useful in various fields such as pharmaceutical products, agricultural products, fibers for clothing, etc.

公开(公告)号：US20180037896A1

公开(公告)日：2018-02-08

申请号：US15545311

申请日：2016-04-11

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Tong Un Chae ,  Chan Woo Song

IPC: C12N15/79 ,  C08G69/14 ,  A61K35/742 ,  C12N15/52

Abstract: The present disclosure relates to a recombinant microorganism having a lactam production capacity from an omega-amino acid, into which a gene encoding a beta-alanine coenzyme A transferase on a microorganism which has an omega-amino acid biosynthetic metabolic pathway inherently or an omega-amino acid biosynthetic metabolic pathway is introduced, and a method for producing a variety of lactams and omega-amino acyl-CoAs using the same.The recombinant microorganism and the method for producing the lactam according to the present disclosure are useful in producing a variety of lactams such as propiolactam, 2-pyrrolidone, valerolactam, caprolactam, and heptanolactam from a variety of omega-amino acids.

公开(公告)号：US20170114345A1

公开(公告)日：2017-04-27

申请号：US15299928

申请日：2016-10-21

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Sang Yup Lee ,  Tong Un Chae

IPC: C12N15/52 ,  C12N9/10 ,  C12N9/88 ,  C12P13/00

CPC classification number: C12N15/52 ,  C12N1/16 ,  C12N1/20 ,  C12N9/1096 ,  C12N9/88 ,  C12P13/001 ,  C12Y206/01 ,  C12Y401/01086

Abstract: The present disclosure relates to a recombinant microorganism producing 1,3-diaminopropane, and a method for producing 1,3-diaminopropane using the same, and specifically, to a recombinant microorganism producing 1,3-diaminopropane into which genes encoding an enzyme involved in a metabolic pathway of 1,3-diaminopropane, dat and ddc, are introduced, and a method for producing 1,3-diaminopropane using the same.When the recombinant microorganism producing the 1,3-diaminopropane according to the present disclosure is used, 1,3-diaminopropane may be mass-produced to be industrially useful in various fields such as pharmaceutical products, agricultural products, fibers for clothing, etc.

公开(公告)号：US12104192B2

公开(公告)日：2024-10-01

申请号：US17194222

申请日：2021-03-06

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Dong In Kim ,  Tong Un Chae

IPC: C12N9/88 ,  C12N1/21 ,  C12P13/00 ,  C12P13/06 ,  C12P13/08

CPC classification number: C12P13/08 ,  C12N9/88 ,  C12P13/001 ,  C12P13/06 ,  C12Y401/01014

Abstract: Disclosed is a method of preparing primary amines. More particularly, disclosed are a mutant microorganism transformed with a gene encoding a valine decarboxylase and a method of preparing primary amines from amino acids using the mutant microorganism. The method has an effect of synthesizing, in an environmentally friendly manner, primary amines as precursors for pharmaceuticals and agricultural chemicals using microorganisms, as an alternative to conventional chemical synthesis methods.

公开(公告)号：US10683512B2

公开(公告)日：2020-06-16

申请号：US15545311

申请日：2016-04-11

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Tong Un Chae ,  Chan Woo Song

IPC: C12N15/52 ,  C12N15/79 ,  C12P17/10 ,  C12N9/10 ,  A61K35/742 ,  C08G69/14

Abstract: The present disclosure relates to a recombinant microorganism having a lactam production capacity from an omega-amino acid, into which a gene encoding a beta-alanine coenzyme A transferase on a microorganism which has an omega-amino acid biosynthetic metabolic pathway inherently or an omega-amino acid biosynthetic metabolic pathway is introduced, and a method for producing a variety of lactams and omega-amino acyl-CoAs using the same.The recombinant microorganism and the method for producing the lactam according to the present disclosure are useful in producing a variety of lactams such as propiolactam, 2-pyrrolidone, valerolactam, caprolactam, and heptanolactam from a variety of omega-amino acids.

公开(公告)号：US10472636B2

公开(公告)日：2019-11-12

申请号：US15556458

申请日：2016-03-17

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Sang Yup Lee ,  Tong Un Chae ,  Chan Woo Song

IPC: C12N1/20 ,  C12N15/52 ,  C12N9/88 ,  C12N15/63 ,  C12P7/40 ,  C12N15/01 ,  C12P7/42 ,  C12P13/00 ,  C12P13/04 ,  C12P13/06 ,  C12R1/00

Abstract: The present invention relates to a mutant organism having the ability to produce aspartic acid derivatives, wherein a gene encoding the glyoxylate shunt regulator and a gene encoding fumarase are deleted and a gene encoding aspartase is overexpressed compared to that in a wild-type strain, and to a method for producing L-aspartic acid derivatives using the same. According to the present invention, various aspartic acid derivatives, including L-alanine, 3-aminopropionic acid, threonine, 1,3-diaminopropane, lysine, methionine, 3-hydroxypropionic acid, cadaverine, 5-aminovaleric acid, etc., can be produced by biological methods.