公开(公告)号：US20070042400A1

公开(公告)日：2007-02-22

申请号：US11429746

申请日：2006-05-08

申请人： K. Choi ,  Michael Zwick ,  Kent Mccue

发明人： K. Choi ,  Michael Zwick ,  Kent Mccue

IPC分类号： C12Q1/68 ,  C12N1/08

CPC分类号： C12Q1/6806 ,  C12N15/10

摘要： Methods of preparing nucleic acid from polysaccharide-containing samples for detection by providing one or more glycosidases to the sample to degrade polysaccharides are provided. The nucleic acids can further be extracted from the sample. The method is particularly useful for detecting nucleic acid in samples with high starch content.

摘要翻译： 提供了通过向样品中提供一种或多种糖苷酶来降解多糖来从含多糖样品中制备核酸以进行检测的方法。 可以从样品中进一步提取核酸。 该方法特别可用于检测具有高淀粉含量的样品中的核酸。

公开(公告)号：US20070231821A1

公开(公告)日：2007-10-04

申请号：US11710667

申请日：2007-02-23

申请人： Charles Bupp ,  K. Choi ,  Rachel Holmes-Davis ,  Alexander Izmailov ,  Heather Koshinsky ,  Christopher Nulf ,  Mickey Urdea ,  Miaomiao Wang ,  Brian Warner ,  Michael Zwick

发明人： Charles Bupp ,  K. Choi ,  Rachel Holmes-Davis ,  Alexander Izmailov ,  Heather Koshinsky ,  Christopher Nulf ,  Mickey Urdea ,  Miaomiao Wang ,  Brian Warner ,  Michael Zwick

IPC分类号： C12Q1/68 ,  C07D417/02 ,  C07D413/02 ,  C07D401/02

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q2600/156

摘要： Methods of determining the presence or amount of a target polynucleotide in a sample are provided. A sample that contains a target polynucleotide, a polynucleotide that may include nucleic acid analogs and that is complementary to a target nucleic acid sequence of the target polynucleotide, and a dye for which the rate of change in an optical property is different in the presence and absence of a target polynucleotide/polynucleotide hybrid are combined to produce a reaction mixture. The rate of change in an optical property of the dye in the reaction mixture is compared to a reference value characteristic of the rate of change in the optical property of the dye in a similar reaction mixture containing a known amount of a polynucleotide/polynucleotide hybrid to determine a relative rate of change in the optical property. The relative rate of change in the optical property of dye in the reaction mixture is correlated with the presence or amount of the specified target polynucleotide in the sample.

摘要翻译： 提供了确定样品中靶多核苷酸的存在或量的方法。 含有靶多核苷酸，可包括核酸类似物并与靶多核苷酸的靶核酸序列互补的多核苷酸的样品和在存在和/或存在的情况下光学性质的变化率不同的染料 组合不存在靶多核苷酸/多核苷酸杂交体以产生反应混合物。 将反应混合物中染料的光学性质的变化率与含有已知量的多核苷酸/多核苷酸杂化物的类似反应混合物中的染料的光学性质变化率的变化率的参考值进行比较 确定光学性质的相对变化率。 反应混合物中染料的光学性质的相对变化率与样品中特定靶多核苷酸的存在或数量相关。

公开(公告)号：US20060147958A1

公开(公告)日：2006-07-06

申请号：US11285025

申请日：2005-11-21

申请人： Heather Koshinsky ,  Michael Zwick ,  K. Choi

发明人： Heather Koshinsky ,  Michael Zwick ,  K. Choi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  Y02A50/52 ,  Y10T436/143333 ,  C12Q2565/107 ,  C12Q2525/113 ,  C12Q2525/107 ,  C12Q2545/113 ,  C12Q2527/125

摘要： The present invention relates to methods for detecting the presence or amount of a target polynucleotide. A polynucleotide, target nucleic acid analog, and dye are combined to form a mixture. The optical property of the dye is observed after the mixture is exposed to a stimulating means. Optionally, after the stimulating means is employed, the mixture is compared to a reference value characteristic of the rate of change in the optical property of the dye in a similar mixture containing a known amount of a target polynucleotide/nucleic acid analog hybrid to determine a relative rate of change in the optical property. The change in a property of the mixture after exposure thereof to a stimulating means or the relative rate of change in the optical property of dye in the mixture is correlated with the presence or amount of the specified target polynucleotide in the sample.

摘要翻译： 本发明涉及用于检测靶多核苷酸的存在或量的方法。 将多核苷酸，靶核酸类似物和染料合并形成混合物。 在将混合物暴露于刺激装置之后，观察染料的光学性质。 任选地，在使用刺激装置之后，将混合物与包含已知量的靶多核苷酸/核酸模拟杂交体的类似混合物中的染料的光学性能的变化率的特性参考值进行比较，以确定 相对光学性质的变化率。 将混合物暴露于刺激装置后的混合物的性质的变化或混合物中染料的光学性质的相对变化率与样品中指定的靶多核苷酸的存在或量相关。