公开(公告)号：US20230417675A1

公开(公告)日：2023-12-28

申请号：US18464624

申请日：2023-09-11

Applicant: Illumina, Inc.

Inventor： Xiuyu CAI ,  Joseph Francis PINTO ,  Thomas A. BAKER ,  Tracy Helen FUNG

IPC: G01N21/64 ,  B01L3/00 ,  G01J1/44 ,  G01N33/58 ,  G02B6/42 ,  H01L27/144 ,  H01L27/146 ,  H01L31/02 ,  H01L31/0216 ,  H01L31/0232

CPC classification number: G01N21/6428 ,  B01L3/502715 ,  B01L3/5085 ,  B01L3/50853 ,  G01J1/44 ,  G01N21/6454 ,  G01N33/582 ,  G02B6/4214 ,  H01L27/1446 ,  H01L27/14609 ,  H01L27/14623 ,  H01L27/14629 ,  H01L31/02019 ,  H01L31/02164 ,  H01L31/02165 ,  H01L31/02327 ,  G01N21/05

Abstract: Light detection devices and related methods are provided. The devices may comprise a reaction structure for containing a reaction solution with a relatively high or low pH and a plurality of reaction sites that generate light emissions. The devices may comprise a device base comprising a plurality of light sensors, device circuitry coupled to the light sensors, and a plurality of light guides that block excitation light but permit the light emissions to pass to a light sensor. The device base may also include a shield layer extending about each light guide between each light guide and the device circuitry, and a protection layer that is chemically inert with respect to the reaction solution extending about each light guide between each light guide and the shield layer. The protection layer prevents reaction solution that passes through the reaction structure and the light guide from interacting with the device circuitry.

公开(公告)号：US20230314326A1

公开(公告)日：2023-10-05

申请号：US18313015

申请日：2023-05-05

Applicant: ILLUMINA, INC.

Inventor： Tracy Helen FUNG ,  Xiuyu CAI ,  Lisa KWOK ,  Hai TRAN ,  Kevan SAMIEE ,  Liangliang QIANG ,  Boyan BOYANOV

IPC: G01N21/64 ,  G01N15/14 ,  G01N21/77 ,  B01L3/00

CPC classification number: G01N21/6454 ,  B01L3/502715 ,  G01N15/1436 ,  G01N21/6486 ,  G01N21/7703 ,  G01N2021/7763

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US20230158494A1

公开(公告)日：2023-05-25

申请号：US18157036

申请日：2023-01-19

Applicant: Illumina, Inc.

Inventor： Dietrich DEHLINGER ,  Ali AGAH ,  Tracy Helen FUNG ,  Emrah KOSTEM ,  Craig HETHERINGTON

IPC: B01L3/00 ,  G01N27/414 ,  G01N21/64 ,  C12Q1/6874 ,  G06F18/24 ,  G06V10/75

CPC classification number: B01L3/502715 ,  G01N27/4145 ,  G01N21/6454 ,  C12Q1/6874 ,  G06F18/24 ,  G06V10/751 ,  B01L2300/0645 ,  B01L2200/147 ,  G01N2021/6471 ,  G01N2021/6482 ,  G01N21/763

Abstract: In one embodiment, a sample surface of a biosensor includes pixel areas and holds a plurality of clusters during a sequence of sampling events such that the clusters are distributed unevenly over the pixel areas. In another embodiment, a biosensor has a sample surface that includes pixel areas and an array of wells overlying the pixel areas, the biosensor including two wells and two clusters per pixel area. The two wells per pixel area include a dominant well and a subordinate well. The dominant well has a larger cross section over the pixel area than the subordinate well. In yet another embodiment, an illumination system is coupled to a biosensor that illuminates the pixel areas with different angles of illumination during a sequence of sampling events, including, for a sampling event, illuminating each of the wells with off-axis illumination to produce asymmetrically illuminated well regions in each of the wells.

公开(公告)号：US20210208072A1

公开(公告)日：2021-07-08

申请号：US17190094

申请日：2021-03-02

Applicant: Illumina, Inc.

Inventor： Xiuyu CAI ,  Joseph Francis PINTO ,  Thomas A. BAKER ,  Tracy Helen FUNG

IPC: G01N21/64 ,  B01L3/00 ,  G01J1/44 ,  G01N33/58 ,  G02B6/42 ,  H01L27/144 ,  H01L27/146 ,  H01L31/02 ,  H01L31/0216 ,  H01L31/0232

Abstract: Light detection devices and related methods are provided. The devices may comprise a reaction structure for containing a reaction solution with a relatively high or low pH and a plurality of reaction sites that generate light emissions. The devices may comprise a device base comprising a plurality of light sensors, device circuitry coupled to the light sensors, and a plurality of light guides that block excitation light but permit the light emissions to pass to a light sensor. The device base may also include a shield layer extending about each light guide between each light guide and the device circuitry, and a protection layer that is chemically inert with respect to the reaction solution extending about each light guide between each light guide and the shield layer. The protection layer prevents reaction solution that passes through the reaction structure and the light guide from interacting with the device circuitry.

公开(公告)号：US20200066684A1

公开(公告)日：2020-02-27

申请号：US16609554

申请日：2017-12-26

Applicant: ILLUMINA, INC.

Inventor： Tracy Helen FUNG ,  Hai Quang TRAN

IPC: H01L25/065 ,  H01L23/00 ,  H01L23/31

Abstract: A system includes an image sensor structure and a flow cell. The image sensor structure includes an image layer disposed over a base substrate. A device stack is disposed over the image layer. A bond pad is disposed in the device stack. A passivation stack is disposed over the device stack and the bond pad. An array of nanowells is disposed in a top layer of the passivation stack. A through-silicon via (TSV) is in electrical contact with the bond pad. The TSV extends through the base substrate. A redistribution layer (RDL) is disposed on a bottom surface of the base substrate. The RDL is in electrical contact with the TSV. The flow cell is disposed upon the top layer of the passivation stack to form a flow channel therebetween. The flow channel is disposed over the array of nanowells and the bond pad.

公开(公告)号：US20250137930A1

公开(公告)日：2025-05-01

申请号：US19010514

申请日：2025-01-06

Applicant: Illumina, Inc.

Inventor： Xiuyu CAI ,  Joseph Francis PINTO ,  Thomas A. BAKER ,  Tracy Helen FUNG

IPC: G01N21/64 ,  B01L3/00 ,  G01J1/44 ,  G01N21/05 ,  G01N33/58 ,  G02B6/42 ,  H10F39/00 ,  H10F39/10 ,  H10F77/00 ,  H10F77/30 ,  H10F77/40

Abstract: Light detection devices and related methods are provided. The devices may comprise a reaction structure for containing a reaction solution with a relatively high or low pH and a plurality of reaction sites that generate light emissions. The devices may comprise a device base comprising a plurality of light sensors, device circuitry coupled to the light sensors, and a plurality of light guides that block excitation light but permit the light emissions to pass to a light sensor. The device base may also include a shield layer extending about each light guide between each light guide and the device circuitry, and a protection layer that is chemically inert with respect to the reaction solution extending about each light guide between each light guide and the shield layer. The protection layer prevents reaction solution that passes through the reaction structure and the light guide from interacting with the device circuitry.

公开(公告)号：US20240014179A1

公开(公告)日：2024-01-11

申请号：US18470900

申请日：2023-09-20

Applicant: Illumina, Inc.

Inventor： Tracy Helen FUNG ,  Hai Quang TRAN

IPC: H01L25/065 ,  H01L23/31 ,  H01L23/00

CPC classification number: H01L25/0657 ,  H01L23/3135 ,  H01L24/29 ,  H01L24/45 ,  H01L24/91 ,  H01L2224/02372 ,  H01L2225/06541

Abstract: A system includes an image sensor structure and a flow cell. The image sensor structure includes an image layer disposed over a base substrate. A device stack is disposed over the image layer. A bond pad is disposed in the device stack. A passivation stack is disposed over the device stack and the bond pad. An array of nanowells is disposed in a top layer of the passivation stack. A through-silicon via (TSV) is in electrical contact with the bond pad. The TSV extends through the base substrate. A redistribution layer (RDL) is disposed on a bottom surface of the base substrate. The RDL is in electrical contact with the TSV. The flow cell is disposed upon the top layer of the passivation stack to form a flow channel therebetween. The flow channel is disposed over the array of nanowells and the bond pad.

公开(公告)号：US20190212295A1

公开(公告)日：2019-07-11

申请号：US16241905

申请日：2019-01-07

Applicant: Illumina, Inc.

Inventor： Dietrich DEHLINGER ,  Ali AGAH ,  Tracy Helen FUNG ,  Emrah KOSTEM ,  Craig HETHERINGTON

IPC: G01N27/414 ,  B01L3/00

CPC classification number: G01N27/4145 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0893 ,  C12Q1/6869 ,  G01N21/6454 ,  G01N21/76 ,  G01N21/763 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2021/6482 ,  G06K9/6202 ,  G06K9/6267

Abstract: In one embodiment, a sample surface of a biosensor includes pixel areas and holds a plurality of clusters during a sequence of sampling events such that the clusters are distributed unevenly over the pixel areas. In another embodiment, a biosensor has a sample surface that includes pixel areas and an array of wells overlying the pixel areas, the biosensor including two wells and two clusters per pixel area. The two wells per pixel area include a dominant well and a subordinate well. The dominant well has a larger cross section over the pixel area than the subordinate well. In yet another embodiment, an illumination system is coupled to a biosensor that illuminates the pixel areas with different angles of illumination during a sequence of sampling events, including, for a sampling event, illuminating each of the wells with off-axis illumination to produce asymmetrically illuminated well regions in each of the wells.

公开(公告)号：US20190212294A1

公开(公告)日：2019-07-11

申请号：US16241902

申请日：2019-01-07

Applicant: Illumina, Inc.

Inventor： Dietrich DEHLINGER ,  Ali AGAH ,  Tracy Helen FUNG ,  Emrah KOSTEM

IPC: G01N27/414 ,  B01L3/00 ,  G06K9/62

CPC classification number: G01N27/4145 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0893 ,  C12Q1/6869 ,  G01N21/6454 ,  G01N21/76 ,  G01N21/763 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2021/6482 ,  G06K9/6202 ,  G06K9/6267

Abstract: A biosensor for base calling is provided. The biosensor comprises a sampling device, which includes a sample surface that has an array of pixel areas and a solid-state imager that has an array of sensors. Each sensor generates pixel signals in each base calling cycle. Each pixel signal represents light gathered in one base calling cycle from one or more clusters in a corresponding pixel area of the sample surface. The biosensor further comprises a signal processor configured for connection to the sampling device. The signal processor receives and processes the pixel signals from the sensors for base calling in a base calling cycle, and uses the pixel signals from fewer sensors than a number of clusters base called in the base calling cycle. The pixel signals from the fewer sensors include at least one pixel signal representing light gathered from at least two clusters in the corresponding pixel area.

公开(公告)号：US20230125640A1

公开(公告)日：2023-04-27

申请号：US18145105

申请日：2022-12-22

Applicant: Illumina, Inc.

Inventor： Xiuyu CAI ,  Joseph Francis PINTO ,  Thomas A. BAKER ,  Tracy Helen FUNG

IPC: G01N21/64 ,  B01L3/00 ,  G01J1/44 ,  G01N33/58 ,  G02B6/42 ,  H01L27/144 ,  H01L27/146 ,  H01L31/02 ,  H01L31/0216 ,  H01L31/0232

Abstract: Light detection devices and related methods are provided. The devices may comprise a reaction structure for containing a reaction solution with a relatively high or low pH and a plurality of reaction sites that generate light emissions. The devices may comprise a device base comprising a plurality of light sensors, device circuitry coupled to the light sensors, and a plurality of light guides that block excitation light but permit the light emissions to pass to a light sensor. The device base may also include a shield layer extending about each light guide between each light guide and the device circuitry, and a protection layer that is chemically inert with respect to the reaction solution extending about each light guide between each light guide and the shield layer. The protection layer prevents reaction solution that passes through the reaction structure and the light guide from interacting with the device circuitry.