公开(公告)号：US11378540B2

公开(公告)日：2022-07-05

申请号：US16877376

申请日：2020-05-18

Applicant: I-SENS, INC.

Inventor： Geun Sig Cha ,  Hakhyun Nam ,  Seok-Won Lee ,  Sung Hyuk Choi ,  Youngjea Kang ,  Myeongho Lee ,  Ho Dong Park ,  Sung Pil Cho

IPC: G01N27/327 ,  G01N33/49 ,  G01N33/487 ,  C12Q1/00 ,  G01N33/543 ,  A61B5/1468 ,  G01N33/53 ,  B01L3/00 ,  A61B5/1495 ,  A61B5/145 ,  G01N27/49 ,  G01N33/26 ,  G01N33/52 ,  G01N27/48 ,  G01N27/42

Abstract: An apparatus for measuring a concentration of an analyte in a bio-sample using an electrochemical bio-sensor, includes a connector with a sample cell in which an oxidation/reduction enzyme and an electron transfer mediator are fixed and a working electrode and an counter electrode are provided; a digital-to-analog converter circuit configured to apply a constant DC voltage to start the oxidation/reduction reaction of the analyte, proceed with an electron transfer reaction, and apply a Λ-step ladder-type perturbation potential for fluctuating a potential of the sample cell after applying the constant DC voltage; and a microcontroller configured to control the digital-to-analog converter circuit and directly obtain a concentration value of the analyte from a calibration equation using the Λ-step ladder-type perturbation potential. The apparatus can improve measurement accuracy by effectively minimizing a matrix interference effect of a background material in a bio-sample, particularly an inaccuracy caused by a change in hematocrit.

公开(公告)号：US10690619B2

公开(公告)日：2020-06-23

申请号：US14564002

申请日：2014-12-08

Applicant: I-SENS, INC.

Inventor： Geun Sig Cha ,  Hakhyun Nam ,  Seok-Won Lee ,  Sung Hyuk Choi ,  Youngjea Kang ,  Myeongho Lee ,  Ho Dong Park ,  Sung Pil Cho

IPC: G01N27/327 ,  G01N33/49 ,  G01N33/487 ,  C12Q1/00 ,  G01N33/543 ,  A61B5/1468 ,  G01N33/53 ,  B01L3/00 ,  A61B5/1495 ,  A61B5/145 ,  G01N27/49 ,  G01N33/26 ,  G01N33/52 ,  G01N27/48 ,  G01N27/42

Abstract: A method for measuring a concentration of an analyte in a bio-sample using an electrochemical bio-sensor according to an exemplary embodiment of the present invention is characterized by a step of obtaining predetermined features from induced currents obtained by applying a DC voltage according to chronoamperometry in which, after a whole blood sample is injected to the electrochemical bio-sensor, a concentration of an analyte is obtained from an induced current obtained by applying a DC voltage for a certain time and from all induced currents obtained by further applying several step-ladder perturbation potentials for a short time subsequent to the DC voltage for the certain time, and is also characterized by minimization of a measurement error caused by a hindering material by forming a calibration equation by combining the at least one feature in a function and optimizing various conditions of the bio-sample through DeletedTextsmultivariable analysis. With this configuration, a perturbation potential application method added to a conventional measurement method can maintain a bio-sensor and a measuring apparatus already used, a line used in the measuring apparatus, and calibration of amperometry as they are, improve accuracy in measurement by effectively minimizing a matrix interference effect of a background material in a bio-sample, particularly an inaccuracy caused by a change in hematocrit, and also remarkably improve accuracy in measurement by simply upgrading a measurement program of a conventional measuring apparatus.