-
公开(公告)号：US09388457B2
公开(公告)日：2016-07-12
申请号：US12211100
申请日：2008-09-15
申请人： Glenn K. Fu , Michael H. Shapero , Pei-Hua Wang
发明人： Glenn K. Fu , Michael H. Shapero , Pei-Hua Wang
CPC分类号： C12Q1/6837 , C12Q1/6844 , C12Q1/6858 , C40B50/06 , C12Q2565/513 , C12Q2537/143 , C12Q2531/113 , C12Q2521/307
摘要： Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. In preferred aspects the analysis is a determination of sequence at one or more locations in the amplified target. The methods may be used for genotyping, sequencing and analysis of copy number.
-
公开(公告)号：US20090117573A1
公开(公告)日：2009-05-07
申请号：US12211100
申请日：2008-09-15
申请人： Glenn K. Fu , Michael H. Shapero , Pei-Hua Wang
发明人： Glenn K. Fu , Michael H. Shapero , Pei-Hua Wang
CPC分类号： C12Q1/6837 , C12Q1/6844 , C12Q1/6858 , C40B50/06 , C12Q2565/513 , C12Q2537/143 , C12Q2531/113 , C12Q2521/307
摘要： Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. In preferred aspects the analysis is a determination of sequence at one or more locations in the amplified target. The methods may be used for genotyping, sequencing and analysis of copy number.
摘要翻译： 提供了用于选择靶的多重放大和扩增靶的分析的方法。 在优选的方面，扩增和分析发生在相同的固体支持物上，优选在局部区域例如珠粒或阵列特征中。 在优选方面，分析是放大靶中一个或多个位置处的序列的确定。 该方法可用于拷贝数的基因分型，测序和分析。
-
公开(公告)号：US08716190B2
公开(公告)日：2014-05-06
申请号：US12899540
申请日：2010-10-06
申请人： Glenn K. Fu , Glenn H. McGall , Robert G. Kuimelis , Jing Hu , Pei-Hua Wang
发明人： Glenn K. Fu , Glenn H. McGall , Robert G. Kuimelis , Jing Hu , Pei-Hua Wang
IPC分类号： C40B30/04
CPC分类号： C12Q1/6837 , C12Q1/6844 , C12Q1/6858 , C12Q1/6874 , C12Q1/6876 , C12Q2600/106 , C12Q2600/154 , C12Q2600/156 , C40B50/06 , C12Q2531/125 , C12Q2565/513 , C12Q2537/143 , C12Q2531/113 , C12Q2521/307
摘要： Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. Targets are circularized by hybridization to probes followed by ligation of the ends of the target to form a closed circle. The targets are then used as template for extension of an array bound probe resulting in extended probes having multiple copies of the target. The extended probes can then be analyzed. The methods may be used for genotyping, sequencing and analysis of copy number.
摘要翻译： 提供了用于选择靶的多重放大和扩增靶的分析的方法。 在优选的方面，扩增和分析发生在相同的固体支持物上，优选在局部区域例如珠粒或阵列特征中。 通过与探针杂交，然后连接靶的末端以形成闭合圆，靶标被循环。 然后将目标用作扩展阵列绑定探针的模板，导致具有多个目标拷贝的扩展探针。 然后可以分析扩展的探针。 该方法可用于拷贝数的基因分型，测序和分析。
-
公开(公告)号：US20110269631A1
公开(公告)日：2011-11-03
申请号：US12899540
申请日：2010-10-06
申请人： Glenn K. Fu , Glenn H. McGall , Robert G. Kuimelis , Jing Hu , Pei-Hua Wang
发明人： Glenn K. Fu , Glenn H. McGall , Robert G. Kuimelis , Jing Hu , Pei-Hua Wang
CPC分类号： C12Q1/6837 , C12Q1/6844 , C12Q1/6858 , C12Q1/6874 , C12Q1/6876 , C12Q2600/106 , C12Q2600/154 , C12Q2600/156 , C40B50/06 , C12Q2531/125 , C12Q2565/513 , C12Q2537/143 , C12Q2531/113 , C12Q2521/307
摘要： Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. Targets are circularized by hybridization to probes followed by ligation of the ends of the target to form a closed circle. The targets are then used as template for extension of an array bound probe resulting in extended probes having multiple copies of the target. The extended probes can then be analyzed. The methods may be used for genotyping, sequencing and analysis of copy number.
摘要翻译： 提供了用于选择靶的多重放大和扩增靶的分析的方法。 在优选的方面，扩增和分析发生在相同的固体支持物上，优选在局部区域例如珠粒或阵列特征中。 通过与探针杂交，然后连接靶的末端以形成闭合圆，靶标被循环。 然后将目标用作扩展阵列绑定探针的模板，导致具有多个目标拷贝的扩展探针。 然后可以分析扩展的探针。 该方法可用于拷贝数的基因分型，测序和分析。
-
公开(公告)号：US5248756A
公开(公告)日：1993-09-28
申请号：US824129
申请日：1992-01-22
申请人： Chih-Chien Hsu , Gerhard K. Guenther , Richele T. Howelton , David E. Bergbreiter , Alan Letton , Pei-Hua Wang
发明人： Chih-Chien Hsu , Gerhard K. Guenther , Richele T. Howelton , David E. Bergbreiter , Alan Letton , Pei-Hua Wang
摘要： Disclosed is an alternating block copolymer composition useful for enhancing the crystallization of bisphenol-A polycarbonate which comprises the reaction product of a polyoxyalkylene polyamine and poly(ethylene)terephthalate. Also disclosed is a method of synthesizing the alternating block copolymer and a method of using the block copolymer composition in the crystallization of bisphenol-A polycarbonate.
摘要翻译： 公开了一种交替的嵌段共聚物组合物，其用于增强双酚A聚碳酸酯的结晶，其包括聚氧化烯多胺和聚对苯二甲酸乙二醇酯的反应产物。 还公开了合成交替嵌段共聚物的方法和在双酚A聚碳酸酯的结晶中使用嵌段共聚物组合物的方法。
-
-
-
-
搜索结果
5 条记录 (耗时 0.024 秒)
