公开(公告)号：US20150307888A1

公开(公告)日：2015-10-29

申请号：US14549962

申请日：2014-11-21

Applicant: Genentech, Inc.

Inventor： Laetitia MALPHETTES ,  Andrew SNOWDEN ,  Inn H. YUK

IPC: C12N15/67

CPC classification number: C12N15/67 ,  C12N15/63 ,  C12N2830/002

Abstract: The invention provides compositions and methods for regulating intracellular osmolarity in cells, e.g., in cultured cells, including in cultured cells in bioreactors. The invention provides nucleic acids comprising at least one osmo-responsive transcriptional regulatory element (OR-TRE), and cells, vectors, products of manufacture, artificial organs or implants and the like containing an osmo-responsive transcriptional regulatory element (OR-TRE).

Abstract translation: 本发明提供用于调节细胞中的细胞内渗透压的组合物和方法，例如在培养的细胞中，包括在生物反应器中的培养细胞中。 本发明提供包含至少一种渗透应答转录调控元件（OR-TRE）的核酸，以及含有渗透应答转录调节元件（OR-TRE）的细胞，载体，制造产物，人造器官或植入物等， 。

公开(公告)号：US20190233790A1

公开(公告)日：2019-08-01

申请号：US16218323

申请日：2018-12-12

Applicant: GENENTECH, INC.

Inventor： Dinesh BASKAR ,  Jenny HSIUNG ,  Woon-Lam Susan LEUNG ,  Inn H. YUK

IPC: C12M1/00 ,  C12M1/34 ,  C12N5/00 ,  C12M1/36 ,  C12M3/06

CPC classification number: C12M23/14 ,  C12M23/28 ,  C12M27/16 ,  C12M29/10 ,  C12M29/20 ,  C12M41/12 ,  C12M41/26 ,  C12M41/32 ,  C12M41/48 ,  C12N5/0018 ,  C12N2500/05

Abstract: An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

公开(公告)号：US20210130759A1

公开(公告)日：2021-05-06

申请号：US17148190

申请日：2021-01-13

Applicant: Genentech, Inc.

Inventor： Dinesh BASKAR ,  Jenny HSIUNG ,  Woon-Lam Susan LEUNG ,  Inn H. YUK

IPC: C12M1/00 ,  C12M3/06 ,  C12M1/34 ,  C12M1/36 ,  C12N5/00

Abstract: An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

公开(公告)号：US20150210971A1

公开(公告)日：2015-07-30

申请号：US14480366

申请日：2014-09-08

Applicant: Genentech, Inc.

Inventor： Dinesh BASKAR ,  Jenny HSIUNG ,  Woon-Lam Susan LEUNG ,  Inn H. YUK

IPC: C12M1/00 ,  C12N5/00 ,  C12M1/36 ,  C12M1/34 ,  C12M3/06

CPC classification number: C12M23/14 ,  C12M23/28 ,  C12M27/16 ,  C12M29/10 ,  C12M29/20 ,  C12M41/12 ,  C12M41/26 ,  C12M41/32 ,  C12M41/48 ,  C12N5/0018 ,  C12N2500/05

Abstract: An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

Abstract translation: 一种在不添加碱的情况下将pH保持在含碳酸氢盐的细胞培养系统中有利于细胞生长的范围内的装置和方法。 该方法依赖于生物反应器系统的气体转移特性来调节往返于细胞培养物的CO 2转移，使得细胞培养物的pH可以保持在所需范围内。